Pooja Suryawanshi, G.M. Vidyasagar, Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts, IJPPE Volume 3, International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 3) https://www.scipress.com/IJPPE.3.84 Abstract: In the present study the antimicrobial activity of chloroform and methanolic extracts of <i>Opuntia</i> <i>cochenillifera</i> for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, <i>E. </i><i>c</i><i>ol</i><i>i</i><i>,</i> <i>B</i><i>acillus</i><i> subtilis</i><i>,</i> <i>Staphyloc</i><i>occus </i><i>aureus</i><i>, </i><i>Pseudomonas </i><i>aeruginosa</i> <i>C</i><i>andida</i> <i>albican</i> <i>C.</i><i>glabrata</i> <i>C.</i><i>h</i><i>aemulonii</i> <i>C</i><i>.</i><i>T</i><i>ropicalis</i><i>.</i> Maximum activity was observed against <i>E. c</i><i>oli</i><i>,</i><i> B. Subtilis</i> <i>and</i><i>,</i> <i>C.albican</i> and <i>C.glabrata</i> at 40mg/ml<i>.</i> Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms. Keywords: Antimicrobial, Fruit, Methanol, <i>Opuntia</i> Cladode