Melappa Govindappa, V. Thanuja, S. Tejashree, C.A. Soukhya, Suresh Barge, Arthikala Manojkumar, Rai V. Ravishankar, In Vitro and In Silico Antioxidant, Anti-Diabetic, Anti-HIV and Anti-Alzheimer Activity of Endophytic Fungi, Cladosporium uredinicola Phytochemicals, IJPPE Volume 13, International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 13)
https://www.scipress.com/IJPPE.13.13
Abstract:
    The present work was aimed to identify phytochemicals in <i>C. </i><i>uredinicola</i> methanol extract from qualitative, TLC and GC-MS method and evaluated for antioxidant, anti-HIV, anti-diabetes, anti-cholinesterase activity <i>in vitro</i> and <i>in </i><i>silico</i>. The <i>C. </i><i>uredinicola</i> extract showed flavonoids, tannins, alkaloids, glycosides, phenols, terpenoids, and coumarins presence in qualitative method. From GC-MS analysis, identified seven different phytochemicals and out of seven, four (coumarin, coumarilic acid, hymecromone, alloisoimperatorin) are coumarins. The <i>C. </i><i>uredinicola</i> extract have shown significant antioxidant activity in DPPH (73) and FRAP (1359) method. The HIV-1 RT (83.81<i>+</i>2.14), gp 120 (80.24<i>+</i>2.31), integrase (79.43<i>+</i>3.14) and protease (77.63+2.14), DPPIV, β-glucosidase and acetyl cholinesterase activity was significantly reduced by the extract. The 2-diphenylmethyleneamino methyl ester had shown significant interaction with oxidant and HIV-1 proteins whereas alloisoimperatorin have interacted with diabetes and cholinesterase proteins followed by hymecromone with high binding energy. These three phytochemicals are non-carcinogens, non-toxic, readily degradable and have drug likeliness properties. The <i>C. </i><i>uredinicola</i> phytochemicals are responsible for management of diabetes, HIV-1 and Alzheimer. Further <i>in vivo</i> work is needed to justify our research.
Keywords:
    Anti-Cholinesterase, Anti-Diabetes, Anti-HIV, Anti-Oxidant, <i>Cladosporium uredinicola</i>