Dattu Singh, Vandana Rathod, Ashish Kumar Singh, Manzoor Ul Haq, Jasmine Mathew, Prema Kulkarni, A Study on Extracellular Synthesis of Silver Nanoparticles from Endophytic Fungi, Isolated from Ethanomedicinal Plants Curcuma longa and Catharanthus roseus, ILNS Volume 57, International Letters of Natural Sciences (Volume 57)
https://www.scipress.com/ILNS.57.58
Abstract:
    Biological method is considered as eco-friendly and reliable process for the synthesis of silver nanoparticles (AgNps) in the field of nanotechnology due to its tremendous applications in various fields. In this study we have isolated a total of twelve endophytic fungi from leaves of <i>Curcuma </i><i>longa</i> (turmeric) and <i>Catharanthus</i> <i>roseus</i> out of which six endophytic fungi showed their ability to synthesized AgNps from silver nitrate (AgNO<sub>3)</sub> solution which splits into a positive silver ion (Ag<sup>+</sup>) and a negative nitrate ion (NO<sub>3</sub><sup>-</sup>) in order to turn the silver ions into solid silver (Ago). Of the six positive endophytic fungi VRD2 showed good and encouraging results and was identified as <i>Penicillium spinulosum</i> VRD2. UV-Visible Spectroscopy confirms the AgNps showing maximum peak at 425nm implying the bioreduction of AgNO<sub>3</sub>. Transmission Electron Microscopy (TEM) revealed the particle are spherical and well dispersed without agglomeration size ranging from 25-30nm.
Keywords:
    <i>Catharanthus roseus</i>, <i>Curcuma longa</i>, <i>Penicillium spinulosum</i>, Silver Nanoparticles, TEM, UV-Visible Spectroscopy