S. Shanmugasundaram, P. Mayavu, T. Manikandarajan, M. Suriya, A. Eswar, R. Anbarasu, Isolation and Identification of Vibrio sp. in the Hepatopancreas of Cultured White Pacific Shrimp (Litopenaeus vannamei), ILNS Volume 46, International Letters of Natural Sciences (Volume 46) https://www.scipress.com/ILNS.46.52 Abstract: The Vibrio is the most common genera with crustaceans often causing various diseases in Aquaculture and significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was carried out, the isolation and identification of pathogenic bacterial flora were isolated from infected in hepatopancreas of <i>vannamei.</i> The SPDS Oceanic farm, RS Aqua farm of and Valli vilas Aqua farm Vellar estuary, Cuddalore District, Tamil Nadu, during the period of (September 2013 to November 2013). The collected samples were plated on TCBS- (Thiosulfate-Citrate-Bile salt-Sucrose) agar medium. The present study, totally 253 green colonies were isolated from TCBS agar plates and among these, 175colonies were identified by using the biochemical tests showed the <i>V. </i><i>parahaemolyticus</i><i>,</i><i> V. </i><i>mimicus</i><i>, V. </i><i>vulnificus</i><i>, V.</i> <i>damsela and P.</i> <i>shigelloides.</i> The maximum species was recorded in <i>V.</i> <i>parahaemolyticus</i> (83.4 %) and minimum was observed in <i>V.</i> <i>mimicus</i> (1.7 %). Keywords: Identification, Pathogens, <i>Penaeus vannamei</i>, <i>Vibrio</i> spp.