N. Sugumar, S. Karthikeyan, T. Gowdhami, Chemical Composition and Antimicrobial Activity of Essential Oil from Eupatorium triplinerve  Vahl. Aerial Parts, ILNS Volume 31, International Letters of Natural Sciences (Volume 31)
https://www.scipress.com/ILNS.31.14
Abstract:
    <i>Eupatorium triplinerve</i> Vahl belongs to the Asteraceae family, popularly known as Ayapana .It is a perennial shrub native to Amazon rainforests of South America. Its leaves are used through infusions, decoctions, baths, and tea. It is largely used in Brazilian folk medicine as sedative, febrifuge, stimulant, tonic and anti-inflammatory. The essential oil from aerial parts of <i>E</i><i>upatorium triplinerve</i> was analysis by gas chromatography mass spectroscopy (GC-MS). Thirty compounds representing 98.24 % of the oil were identified. The major compounds were 2-tert-butyl – 1, 4-methoxybenzene (74.3 %) and b –Selinene (8.6 %). The antibacterial activity of the essential oil was evaluated against ten bacteria (including Gram positive and Gram negative) and six phytopathogenic fungi. The oil exhibited moderated antibacterial and strong antifungal activity against all the test pathogens. The highest zone inhibition was recorded against <i>Salamella typ</i><i>hae</i> (21mm) followed by <i>Shigella sonnei</i> (18mm) at a concentration of 20μl/ disc on the other standard <i>Ampicillin</i> 20mg/ disc showed on average of 14-34mm diameter of zone inhibition against the test organism. The lowest antifungal activities were recorded against <i>Macrophomina phaseoline</i>, and <i>Botryodiplodia theobromae</i>. The highest <i>Alternaria alternate</i> (12000ppm).Further pharmacological and toxicology studies will be required to establish the therapeutic uses of this oil.
Keywords:
    2-Tert-Butyl-1, 4-Methoxybenzene, Antimicrobial Activity, Essential Oil, <i>Eupatorium triplinerve</i>, GC-MS