Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

JHPR > Volume 3 > Sex Determination in Nutmeg Seedlings Using...
Sex Determination in Nutmeg Seedlings Using Scar Primers
< Back to Volume

Sex Determination in Nutmeg Seedlings Using Scar Primers

Full Text PDF

Abstract:

Myristica fragrans H. is a dieocious plant with male and female flowers on different trees. At the juvenile stage of nutmeg, their sexes is not morphologically identified until at the flowering stage. However, the female is more economical than the male plants. This challenge is making the work of nutmeg farmers difficult and also inhibiting the development of the spice and flavouring industry in Ghana. Hence, the use of molecular technique to characterize male and female nutmeg plants. SCAR markers are easy, specific, and reliable and have a high sense of reproducibility than other markers. Two different primers (F-Napf-76, R-Napf-77 and F-Napf-70, R-Napf-71) were used for the PCR amplification. It was revealed that the total genomic DNA of samples from both mature plant and seedlings of nutmeg were of good quality and was much concentrated by giving good DNA bands. Although there were no distinctions among the bands, they all lie at the same level (0.3kbp) indicating that the samples were of similar molecular weight. However, double PCR amplification was not seen in all samples when run on a 1% agarose gel but single amplifications were observed.

Info:

Periodical:
Journal of Horticulture and Plant Research (Volume 3)
Pages:
40-47
DOI:
https://doi.org/10.18052/www.scipress.com/JHPR.3.40
Citation:
F. M. Dadzie, "Sex Determination in Nutmeg Seedlings Using Scar Primers", Journal of Horticulture and Plant Research, Vol. 3, pp. 40-47, 2018
Online since:
August 2018
Authors:
Francis Mintah Dadzie
Keywords:
Nutmeg, SCAR Primers, Sex Determination
Export:
RIS, BibTeX, Text
Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] M. Anandaraj et al., Nutmeg. Extension Pamphlet, II SR, (2005).

[2] United States Department of Agriculture (USDA, 2010). National Genetic Resources Program.

[3] D. Dhingra, A. Sharma, Antidepressant- like activity of n-hexane extract of nutmeg (Myristica fragrans Houtt.) seeds in mice, Journal of Medical Food. 9 (2006) 84-89.

DOI: https://doi.org/10.1089/jmf.2006.9.84

[4] J. Asgarpanah, N. Kazemivash, Phytochemistry and pharmacological properties of Myristica fragrans Houtt.: A review, African Journal of Biotechnology. 11(65) (2012) 12787-12793.

DOI: https://doi.org/10.5897/ajb12.1043

[5] U. Stein, H. Greyer, H. Hentschel, Nutmeg (Myristicin) poisoning- report on fatal case and a series of cases recorded by a poison information centre, Forensic Science International. 118(1) (2001) 87-90.

DOI: https://doi.org/10.1016/s0379-0738(00)00369-8

[6] K. Jangid, N.D. Jayakumar, S.S. Varghese, Achievable therapeutic effects of Myristica fragrans (Nutmeg) on periodontitis - a short review, International Journal of Pharmacy and Pharmaceutical Sciences. 6(5) (2014) 591-594.

[7] B. Yakubov, O. Barazani, A.G. Goldhirish, Combination of SCAR primers and touchdown PCR for sex identification in Pistachia vera L., Scientia Horticulturae. 103 (2005) 473-478.

DOI: https://doi.org/10.1016/j.scienta.2004.06.008

[8] N. Urazaki et al., A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.), Theoretical and Applied Genetics. 2 (2002) 281-285.

DOI: https://doi.org/10.1007/s001220100693

[9] A.S. Parasnis et al., A highly reliable sex diagnostic PCR assay for mass screening of papaya seedlings, Molecular Breeding. 6 (2000) 337-344.

[10] J.W. Lee et al., Development of an ISSR derived SCAR marker in Korean ginseng cultivar (Panax ginseng C.A. Meyer), Journal of Ginseng Research. 35(1) (2011) 52-59.

DOI: https://doi.org/10.5142/jgr.2011.35.1.052

[11] P. Manoj, N.S. Banerjee, P. Ravichandran, Development of sex specific molecular markers in dioecious Piper Longum L. plants by differentiated display, Journal of Theoretical and Applied Information Technology. 4(5) (2008) 459-465.

[12] M.S. Vinod et al., Identification of a sex-specific SCAR marker in dioecious Pandanus fascicularis L. (Pandanaceae), Genome. 50 (2007) 834-839.

DOI: https://doi.org/10.1139/g07-066

[13] K.M. Devaiah, P. Venkatasubramanian, Development of SCAR marker for authentication of Pueraria tuberose (Roxb. ex. Willd.) DC, Current Science. 94(10) (2008) 1306-1308.

[14] P. Hernandez, A. Martin, G. Dorado Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker-assisted selection of wheat, Molecular Breeding. 5(3) (1999) 245-253.

[15] S.S. Renner, R.E. Ricklefs, Dioecy and its correlation in the flowering plants, American Journal of Poot. 82 (1995) 596-606.

[16] C. Ainsworth, Boys and girls come out to play: the molecular biology of dioecious plants, Annals of Botany. 86 (2000) 211-221.

DOI: https://doi.org/10.1006/anbo.2000.1201

[17] D. Charlesworth, Plant sex determination and sex chromosomes, Heredity. 88 (2002) 94-101.

DOI: https://doi.org/10.1038/sj.hdy.6800016

[18] S. Matsunaga, S. Kawano, Sex determination by sex chromosomes in dioecious plants, Plant Biology. 3 (2001) 481-488.

DOI: https://doi.org/10.1055/s-2001-17735

[19] I. Stehlik, F.R. Blattner, Sex Specific SCAR markers in the dioecious plant Rume nivalis (Polygonacae) and implications for the evolution of sex chromosomes, Theoretical Applied Genetics. 108 (2004) 238-242.

DOI: https://doi.org/10.1007/s00122-003-1425-7

[20] N.C. Oraguize et al., Comparison of RAPD and morpho-nut markers for revealing genetic relationships between Chestnut sp. (Castenea sp.) and New Zealand chestnut selections, New Zealand Journal of Crops Horticultural Science. 26 (1998) 109-115.

DOI: https://doi.org/10.1080/01140671.1998.9514047

[21] B. Krishnamoorthy, B. Sasikumar, J. Rema, Genetic variability and segregation of sex in nutmeg (Myristica fragrans Houtt.), Journal of Plantation Crops. 24 (1996) 468-472.

[22] T.E. Sheeja et al., DNA polymorphism in clonal and seedlings progeny of an elite nutmeg (Myristica fragrans Houtt.), Journal of Plantation Crops. 34(2) (2006) 558-561.

[23] N.A. Phadnis, K.G. Choudhary, Sex determination in the seedling stage of nutmeg (Myristica fragrans, Houtt), Tropical Science. 13(4) (1971) 265-274.

[24] B.K. Nayar, R. Rajendra, P. Vatsala, A simple morphological technique distinguish the sex of nutmeg seedlings, Current Science. 46(5) (1976) 156-157.

[25] E.V. Packiyasothy, E.R. Jansz, H.M. Dharmadasa, Studies on some chemical components of nutmeg (Myristica fragrans Houtt.) leaf directed at determination of sex in seedlings, Journal of the National Science Foundation of Sri Lanka. 19(2) (1991).

DOI: https://doi.org/10.4038/jnsfsr.v19i2.8156

[26] N. Menzencev et al., Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants, Journal of Genetics and Breed. 51 (1997) 97-102.

[27] S. Forapani et al., RAPD markers for potato germplasm characterization, Journal of Genetics and Breed. 53 (1999) 143-147.

[28] S. Kafkas, S. Cetiner, R. Perl-Treves, Development of sex associated RAPD markers in wild Pistacia sp., Journal of Horticultural Science and Biotechnology. 76 (2001) 242-246.

DOI: https://doi.org/10.1080/14620316.2001.11511358

[29] P.S. Virk et al., Use of RAPD for the study of diversity within the Plant Germplasm collections, Heredity. 74 (1995) 170-179.

DOI: https://doi.org/10.1038/hdy.1995.25

[30] J.O. Olufowote et al., Comparative evaluation within cultivar variation of rice (Oryzae sativa L.) using microsatellite and RFLP markers, Genome. 40 (1997) 370-378.

DOI: https://doi.org/10.1139/g97-050

[31] E.G.M. Lemos, C.L.S.P. Silva, H.A. Zaidan, Identification of sex in Carica papaya L. using RAPD markers, Euphytica. 127 (2003) 179-184.

[32] K.N. Ganeshaiah et al., Identification of sex sex specific DNA marker in the dioecious tree (nutmeg), Plant Genetic Resource Newsletter. 121 (2000) 59-61.

[33] C. Takrama, CTAB Protocol for Plant DNA extraction, (2000).

[34] A.S. Parasnis et al., A Highly Reliable Sex Diagnostic PCR Assay for mass Screening of Papaya seedlings, Molecular Breeding. 6 (2004) 337-344.

[35] R.A.A. Younis et al., Identification of sex-specific DNA markers for date palm (Phoenix dactylifera L.) using RAPD techniques, Research Journal of Agriculture and Biological Science. 4(4) 2008) 278-284.

[36] K.C. Sink, C. Jiang, RAPD and SCAR markers linked to the sex expression locus M in asparagus, Euphytica. 94(3) (1997) 329-333.

[37] J.S. Parker, Sex chromosomes and sexual differentiation in flowering plants, Chromosomes Today. 10 (1990) 187-198.

[38] M. Flach, Nutmeg cultivar and sex problems, Eng. Summ. Meded. Landb. Hogesh. 66 (1966) 1.

[39] E. Niroshini et al., Detection of SCAR marker linked to sex expression in Carica papaya L., Journal of the National Science Foundation of Sri Lanka. 36(2) (2008) 145-150.

DOI: https://doi.org/10.4038/jnsfsr.v36i2.146

[40] T.E. Sheeja et al., Optimization of DNA isolation and PCR parameters in Myristica sp. and related genera for RAPD and ISSR analysis, Journal of Spices and Aromatic Crops. 17(2) (2008) 91-97.

Show More Hide
Cited By:
This article has no citations.