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Sex Determination in Nutmeg Seedlings Using Scar Primers

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Abstract:

Myristica fragrans H. is a dieocious plant with male and female flowers on different trees. At the juvenile stage of nutmeg, their sexes is not morphologically identified until at the flowering stage. However, the female is more economical than the male plants. This challenge is making the work of nutmeg farmers difficult and also inhibiting the development of the spice and flavouring industry in Ghana. Hence, the use of molecular technique to characterize male and female nutmeg plants. SCAR markers are easy, specific, and reliable and have a high sense of reproducibility than other markers. Two different primers (F-Napf-76, R-Napf-77 and F-Napf-70, R-Napf-71) were used for the PCR amplification. It was revealed that the total genomic DNA of samples from both mature plant and seedlings of nutmeg were of good quality and was much concentrated by giving good DNA bands. Although there were no distinctions among the bands, they all lie at the same level (0.3kbp) indicating that the samples were of similar molecular weight. However, double PCR amplification was not seen in all samples when run on a 1% agarose gel but single amplifications were observed.

Info:

Periodical:
Journal of Horticulture and Plant Research (Volume 3)
Pages:
40-47
Citation:
F. M. Dadzie, "Sex Determination in Nutmeg Seedlings Using Scar Primers", Journal of Horticulture and Plant Research, Vol. 3, pp. 40-47, 2018
Online since:
August 2018
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References:

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