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JHPR > Volume 3 > In Vitro Regeneration of Medicinally Important...
<i>In Vitro</i> Regeneration of Medicinally Important Shrub <i>Carissa Opaca</i> from Shoot Apices and Nodal Segments
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In Vitro Regeneration of Medicinally Important Shrub Carissa Opaca from Shoot Apices and Nodal Segments

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Abstract:

The study was aimed to develop efficient shoot regeneration from ex vitro explants of Carissa opaca, an imperative medicinal reservoir. Shoot apices and nodal segments were inoculated on MS (Murashige and Skoog) medium containing BAP (6-bezyl amino purine) and Kin (Kinetin) alone and in combination with NAA (naphthalene acetic acid) and GA3 (Gibberellic acid). Higher concentrations of both cytokinins were found effective for regeneration from both explants. However, gibberellic acid and NAA addition with cytokinin, no persuading results were achieved. The shoot apices were found more effective in in vitro regeneration than nodal segments.The protocol can be effectively used for in vitro multiplication of C. opaca, genetic transformation, and secondary metabolite production.

Info:

Periodical:
Journal of Horticulture and Plant Research (Volume 3)
Pages:
23-29
DOI:
https://doi.org/10.18052/www.scipress.com/JHPR.3.23
Citation:
A. Ahmad et al., "In Vitro Regeneration of Medicinally Important Shrub Carissa Opaca from Shoot Apices and Nodal Segments", Journal of Horticulture and Plant Research, Vol. 3, pp. 23-29, 2018
Online since:
August 2018
Authors:
Ali Ahmad, Bilal Haider Abbasi, Muhammad Zia
Keywords:
Carissa opaca, Cytokinin, In Vitro Regeneration, Medicinal Plant, MS Media, Plant Growth Regulators, Shoot Regeneration
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Distribution:
Open Access

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This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] A.M. Abbasi et al., Ethnobotanical study of wound healing herbs among the tribal communities in Northern Himalaya Ranges District Abbottabad, Pakistan, Pak. J. Bot. 6 (2010) 3747-3753.

[2] S. Sahreen et al., Hepatoprotective effects of methanol extract of Carissa opaca leaves on CCl 4-induced damage in rat, BMC Com. Alt. Med. 11(1) (2011) 48.

DOI: https://doi.org/10.1186/1472-6882-11-48

[3] S. Izhar, D. Ahmed, Carissa opaca: A plant with great potential for future drugs for degenerative and infectious diseases, Chem Select. 1(12) (2016) 3005-3011.

DOI: https://doi.org/10.1002/slct.201600462

[4] S. Prakash, J. Van Staden, Micropropagation of Hoslundia opposita Vahl – a valuable medicinal plant, South African J. Bot. 73 (2007) 60-63.

DOI: https://doi.org/10.1016/j.sajb.2006.07.001

[5] Y. Sidhu, In vitro micropropagation of medicinal plants by tissue culture, The Plymouth Student Scientist. 4 (2011) 432-449.

[6] T. Murashige, F. Skoog, A revised medium for rapid growth and bio assays with tobacco tissue cultures, Physiologia Plantarum. 15(3) (1962) 473-497.

DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

[7] M.S. Rathore et al., Micropropagation of elite genotype of Jatropha curcas L. through enhanced axillary bud proliferation and ex vitro rooting, Biomass and Bioenergy. 83 (2015) 501-510.

DOI: https://doi.org/10.1016/j.biombioe.2015.10.023

[8] A.K. Patel et al., In vitro propagation and ex vitro rooting of Caralluma edulis (Edgew.) Benth. & Hook. f.: an endemic and endangered edible plant species of the Thar Desert, Scientia Horticulturae. 165 (2014) 175-180.

DOI: https://doi.org/10.1016/j.scienta.2013.10.039

[9] R.U. Rehman et al., In vitro propagation of chungah (Caralluma tuberculata NE Brown), Pak. J. Bot. 46 (2014) 2189-2194.

[10] M.A. Imran et al., Effect of adenine sulphate (ads) with cytokinins on multiple shoot production in Carissa carandas (L.), International Journal of Pharma and Bio Sciences. 3(1) (2012) 473-480.

[11] R. Rai, K.K. Misra, Micropropagation of Karonda (Carissa carandas) through shoot multiplication, Scientia Horticulturae. 103(2) (2005) 227-232.

DOI: https://doi.org/10.1016/j.scienta.2003.09.005

[12] A.P. Costa et al. Branching, flowering and fruiting of Jatropha curcas treated with ethephon or benzyladenine and gibberellins, Anais da Academia Brasileira de Ciências. 88 (2016) 989-998.

DOI: https://doi.org/10.1590/0001-3765201620140635

[13] A. Varshney, M. Anis, Trees: propagation and conservation: biotechnological approaches for propagation of a multipurpose tree, Balanites aegyptiaca Del, Springer Science & Business Media, (2014).

[14] S.N. Hasmah, A. Bhatt, C.L. Keng, Micropropagation of Asam Karanda (Carissa carandas Linn), Pertanika Journal of Tropical Agricultural Science. 36(1) (2013) 89-98.

[15] E.E. Benson, Sepecial symposium: In vitro plant recalcitrance in vitro plant recalcitrance: An introduction, In Vitro Cell. Dev. Biol. Plant. 36 (2000) 141-148.

DOI: https://doi.org/10.1007/s11627-000-0029-z

[16] P.T. Lynch, Tissue culture techniques in in vitro plant conservation, in: Plant Conservation Biotechnology, 2014, pp.67-68.

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