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JHPR > JHPR Volume 3 > In Vitro Regeneration of Medicinally Important...
<i>In Vitro</i> Regeneration of Medicinally Important Shrub <i>Carissa Opaca</i> from Shoot Apices and Nodal Segments
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In Vitro Regeneration of Medicinally Important Shrub Carissa Opaca from Shoot Apices and Nodal Segments

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Abstract:

The study was aimed to develop efficient shoot regeneration from ex vitro explants of Carissa opaca, an imperative medicinal reservoir. Shoot apices and nodal segments were inoculated on MS (Murashige and Skoog) medium containing BAP (6-bezyl amino purine) and Kin (Kinetin) alone and in combination with NAA (naphthalene acetic acid) and GA3 (Gibberellic acid). Higher concentrations of both cytokinins were found effective for regeneration from both explants. However, gibberellic acid and NAA addition with cytokinin, no persuading results were achieved. The shoot apices were found more effective in in vitro regeneration than nodal segments.The protocol can be effectively used for in vitro multiplication of C. opaca, genetic transformation, and secondary metabolite production.

Info:

Periodical:
Journal of Horticulture and Plant Research (Volume 3)
Pages:
23-29
DOI:
https://doi.org/10.18052/www.scipress.com/JHPR.3.23
Citation:
A. Ahmad et al., "In Vitro Regeneration of Medicinally Important Shrub Carissa Opaca from Shoot Apices and Nodal Segments", Journal of Horticulture and Plant Research, Vol. 3, pp. 23-29, 2018
Online since:
August 2018
Authors:
Ali Ahmad, Bilal Haider Abbasi, Muhammad Zia
Keywords:
Carissa opaca, Cytokinin, In Vitro Regeneration, Medicinal Plant, MS Media, Plant Growth Regulators, Shoot Regeneration
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Distribution:
Open Access

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This work is licensed under a
Creative Commons Attribution 4.0 International License

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