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Arbuscular Mycorrhizal Fungi (AMF) as Buffer for Heavy Metals Phytoextraction by Cucurbita maxima Duch. Grown on Crude Oil Contaminated Soil

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This study evaluated the influence of Arbuscular Mycorrhizal (Rhizophagus irregularis) fungi inoculation (M) on the growth of Cucurbita maxima and as a buffer against phytoextraction of selected heavy metals (HM) (Zn, Cu, Cr, Cd and Pb) from a soil contaminated with crude oil (C). The experiment was set up using four soil treatments, each with three replicates C+ M-, C+ M+, C-M+ and C-M- (control without oil and inoculum). The shoot length, petiole length, number of nodes, leaf area and percentage germination of C. maxima were significantly (p=0.05) reduced in uninoculated crude oil treatment (C+ M-), unpolluted mycorrhizal inoculated treatments (C-M+) showed remarkable response in growth parameters above the control (C-M-), while the polluted and inoculated treatment (C+ M+) showed significant (p=0.05) increase in growth parameters when compared to the polluted uninoculated treatment (C+ M-). Heavy metals analysis revealed a significant (p=0.05) difference in the heavy metal accumulation of C. maxima. The heavy metals analyzed in this study are present thus in C. maxima; Zn>Cu>Cr>Pb>Cd. Crude oil polluted uninoculated treatment (C+ M-) recorded the highest concentrations of heavy metals than crude oil polluted inoculated (R. irregularis) treatment (C+ M+). Mycorrhizal inoculated unpolluted treatment (C-M+) and unpolluted uninoculated treatment (C-M-) indicated the lowest heavy metal concentrations. Inoculation with R. irregularis significantly (p=0.05) reduced heavy metals uptake by C. maxima as observed in this study. Also, the negative effect of crude oil on AMF root colonization of C. maxima by R. irregularis was observed in polluted and inoculated treatment. HM often accumulate in the top layer of soil, therefore, are available for uptake by plants via roots, which is a major entry point of HM that ultimately affects different physiological processes. AM fungi can impinge on the chemical properties of heavy metals in the soil, their absorption by the host plant, and their allocation to different plant parts, affecting plant growth and the bioremediation process, thus making the AM fungi a suitable buffer for mitigating heavy metal stress on C. maxima.


Journal of Horticulture and Plant Research (Volume 3)
O. G. Okon et al., "Arbuscular Mycorrhizal Fungi (AMF) as Buffer for Heavy Metals Phytoextraction by Cucurbita maxima Duch. Grown on Crude Oil Contaminated Soil", Journal of Horticulture and Plant Research, Vol. 3, pp. 1-12, 2018
Online since:
August 2018

[1] S.S. Gill, N. Tuteja, Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants, Plant Physiol. Biochem. 48 (2010) 909-930.


[2] M. Miransari, Metal tolerance in plants, in: Q.S. Wu (Ed.), Arbuscular Mycorrhizas and Stress Tolerance of Plants, Isfahan, Iran, AbtinBerkeh Scientific Ltd. Company. 2017, pp.147-158.


[3] K.K.A. Abdul, O.L. Mojeed, J.P. Oladele, Effect of mycorrhizal inoculation on the growth and phytoextraction of heavy metals by maize grown in oil contaminated soil, Pakistani Journal of Botany. 44(1) (2012) 221-230.

[4] A. Holleman, E. Wiberg, Lehrbuch der Anorganischen Chemie, Nabu Press, Berlin, (1985).

[5] A. Jamal, N. Ayuba, M. Usmana, Arbuscular mycorrhizal fungi enhance zinc and nickel uptake from contaminated soil by soyabean and lentil. International Journal of Phytoremediation, 4 (2002) 205–221.


[6] M. Friedlova, The influence of heavy metals on soil biological and chemical properties. Soil Water Res 5 (2010) 21–27.

[7] A. Whitmore, The emperors new clothes: sustainable mining? J. Clean Prod. 14 (2006) 309-314.

[8] A. Schübler, C. Walker, The Glomeromycota. A species list with new families and new genera. ( Accessed: 20 March (2015).

[9] S.E. Smith, D.J. Read, Mycorrhizal symbiosis (3rd ed.), A San Diego: Academic, (2008).

[10] A. Franco-Ramírez et al., Arbuscular mycorrhizal fungi in chronically petroleum contaminated soils in Mexico and the effects of petroleum hydrocarbons on spore germination, Journal of Basic Microbiology. 47 (2007) 378-383.


[11] C. Nardini, L. Di Salvo, D.S.I. García, Micorrizas arbusculares: asociaciones simbióticas e indicadores de calidad ambiental en sistemas de cultivos extensivos, Revista Argentina de Microbiología. 43 (2011) 311-312.

[12] T. Pawlowska, I. Charvat, Heavy-metal stress and developmental patterns of arbuscular mycorrhizal fungi, Appl. Environ. Microbiol. 70 (2004) 6643-6649.


[13] M. Miransari, Hyperaccumulators, arbuscular mycorrhizal fungi and stress of heavy metals, Biotechnol. Adv. 29 (2011) 645-653.


[14] I.M. Villasenor, A.L.O. Bartolome, Microbiological and pharamcological studies on extracts of Cucurbita maxima, Phytotherapy research. 9(5) (1995) 376-378.

[15] F.C. Ngwerume, G.J.H. Grubben, Cucurbita maxima Duchesne. Record from PROTA4U. Grubben, G. J. H. and Denton, O. A. (Editors). PROTA (Plant Resources of Tropical Africa / Ressources végétales de l'Afrique tropicale), Wageningen, Netherlands. (2004).

[16] B.E. Okoli, Wild and cultivated cucurbits in Nigeria, Economic Botany. 38(3) (1984) 350-357.


[17] I.O. Agbagwa, B.C. Ndukwu, Cucurbita L. Species in Nigeria: under-exploited food and vegetable crops, Niger Delta Biological. 4(2) (2004) 11-15.

[18] G.J. Esenowo, N.S. Umoh, The effect of used engine oil pollution of soil on the growth and yield of Arachis hypogea L. and Zea mays L., Transactions of Nigerian Society of Biological Conservation. 5 (1996) 71-79.

[19] Association of Official Analytical Chemists (AOAC), Official Methods of Analysis. (17th Edn.) Arlington, Virginia: AOAC, 2003, pp.96-105.

[20] C. Walker, A simple blue staining technique for arbuscular mycorrhizal and other root-inhibiting fungi, Inoculum. 56(4) (2005) 68-69.

[21] M. Giovannetti, B. Mosse, An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots, New Phytologist. 84(3) (1980) 489-500.


[22] I.A. Ekpo et al., Effect of crude oil polluted soil on germination and growth of soybean (Glycine max), Annals of Biological Research. 3(6) (2012) 3049-3054.

[23] S.A. Olusola, E.E. Anslem, Bioremediation of a crude oil polluted soil with Pleurotus Pulmonarius and Glomus Mosseae using Amaranthus Hybridus as a test plant, Journal Bioremed. Biodegrad. 1 (2010) 113.


[24] M.O. Liasu, M.A. Azeez, M.O. Atayese, Influence of AM fungal inoculation on performance of three cultivars of maize during late seasons in a savanna soil, Science Focus. 2 (2002) 62-66.

[25] J.M. Ayotamuno, R.B. Kogbara, Determining the tolerance level of Zea mays (maize) to a crude oil polluted agricultural soil, Afr. J. Biotech. 6(11) (2007).

[26] E.M. Ogbo, A. Tabuanu, R. Ubebe, Phytotoxicity assay of diesel fuel-spiked substrates remediated with Pleurotus tuberregium using Zea mays, Int. J. Appl. Res. Natur. Prod. 3(2) (2010) 12-16.

[27] M.O. Liasu, The effect of Glomus mosseae Nicholsen Gerdmann on establishment and growth performance of transplanted potted tomato plants in soils supplemented with composted brewery waste, American-Eurasian J. Sust. Agric. 2(2) (2008) 125-134.

[28] O.G. Okon, Bioaccumulation of heavy metals in Cucurbita maxima Duch. and Telfairia occidentalis Hook. F. grown on crude oil polluted soil, American Journal of Agricultural Science. 4(4) (2017) 88-93.

[29] O.M. Agbogidi, P.G. Eruotor, S.O. Akparabi, Effects of time of application of crude oil to soil on the growth of maize (Zea mays L.), Research Journal of Environmental Toxicology. 1(3) (2007) 116-123.


[30] E. Epstein, Mineral Nutrition of plants, principles and perspective, New York, John Willey and Sons Incorporated, 1972, p.412–414.

[31] H.C. Ogbuehi, I.O. Ezeibekwe, M.C. Ejiogu, Impact of spent engine oil pollution on the proximate composition and accumulation of heavy metals in groundnut (Arachis hypogea) grown in Owerri, Imo State, Nigeria, Global Journal of Science. 3(12) (2011).

[32] H.C. Ogbuehi, L.A. Akonye, Phytoextractive ability cassava cultivars to accumulate heavy metal in crude contaminated soil, International Science Research Journal. 1(1) (2008) 18-21.

[33] H.C. Ogbuehi, I.O. Ezeibekwe, U. Agbakwuru, Assessment of crude oil pollution the proximate composition and macro element of cassava crop in Owerri, Imo State, International Science Research Journal. (2) (2010) 62-65.

[34] S. Dushekove et al., Phytoremediation a novel approach to an old problem, in: Global Environment Biotechnology Proceedings of the Third Biennial Meeting of the International Society for Environmental Biotechnology 15-20. July, 1996, Boston M. A. Elsevier, New York, 1997, p.563.

[35] E.O. Ekundayo, T.O. Emede, D.J. Osayande, Effects of crude oil spillage on growth and yield of maize (Zea mays L.) in soil of Midwestern Nigeria, Plant Food for Human Nutrition. 56(4) (2001) 313-324.


[36] A.N. Chukwuemeka, J.U. Godwin, O.N. Alfredo, Investigation of heavy metals concentration in leaves of Telfaira occidentalis Hook. F. (Fluted Pumpkin) in Nigeria, Pol. J. Environ. Stud. 24(4) (2015) 1733-1742.


[37] C.I. Osu, E.C. Ogoko, Bioconcentration and transfer of heavy metal from soil into Verninia amydalina, Telfaira occidentalis and Amaranthus spinosus, Journal of Applied Phytotechnology in Environmental Sanitation. 3(4) (2014) 117.

[38] S. Batra, Importance of trace elements in the human body, Food and Nutrition. 5(1) (2012) 45-48.

[39] M.O. Liasu, A.F. Ogundola, M.O. Atayese, Influence of mycorrhizal inoculation on growth and development of potted tomato in a soil contaminated with spent oil, Science Focus, 12(1) (2006) 59-64.

[40] J. Schneider, J. Bundschuh, C. do Nascimento, Arbuscular mycorrhizal fungi-assisted phytoremediation of a lead-contaminated site, Sci. Total Environ. 572 (2016) 86–97.


[41] M. Hristozkova et al, Aspects of mycorrhizal colonization in adaptation of sweet marjoram (Origanum majorana L.) grown on industrially polluted soil, Turk. J. Biol. 39 (2015) 461–468.


[42] U. Galli, H. Schuepp, C. Brunold, Thiol of Cu-treated maize plants inoculated with the arbuscular-mycorrhizal fungus Glomus intraradices, Physiol. Plant. 94 (1995) 247-253.


[43] E.F. Abd-Allah et al., Alleviation of adverse impact of cadmium stress in sunflower (Helianthus annuus L.) by arbuscular mycorrhizal fungi, Pak. J. Bot. 47 (2015) 785–795.

[44] L. Cabral et al., Retention of heavy metals by arbuscular mycorrhizal fungi mycelium, Química Nova. 33 (2010) 25–29.

[45] S. Gianinazzi et al., Mycorrhizal technology, in: Agriculture, Birkhăuser Nerlag, Basel–Boston–Berlin, (2002).

[46] M.M. Lasat, Phytoextraction of toxic metals: A review of biological mechanisms, J. Environ. Quality. 31 (2002) 109-120.

[47] S. Wu et al., Chromium immobilization by extra-and intraradical fungal structures of arbuscular mycorrhizal symbioses, J. Haz. Mat. 316 (2016) 34-42.

[48] S. Kanwal, A. Bano, R. N. Malik, Role of arbuscular mycorrhizal fungi in phytoremediation of heavy metals and effects on growth and biochemical activities of wheat (Triticum aestivum L.) plants in Zn contaminated soils, Afr. J. Biotechnol. 15 (2016).


[49] F. Wang, X. Lin, R. Yin, Heavy metal uptake by arbuscular mycorrhizas of Elsholtzia splendens and the potential for phytoremediation of contaminated soil, Plant Soil. 269 (2005) 225-232.


[50] F. Wang, X. Lin, R. Yin, Role of microbial inoculation and chitosan in phytoextraction of Cu, Zn, Pb and Cd by Elsholtzia splendens—a field case, Environ. Pollut. 147 (2007) 248-255.


[51] W. Yang et al., Metal removal from and microbial property improvement of a multiple heavy metals contaminated soil by phytoextraction with a cadmium hyperaccumulator Sedum alfredii H., J. Soils Sedim. 14 (2014) 1385-1396.


[52] N.S. Udo, O.G. Okon, Heavy metal concentrations of citrus species (Citrus reticulata and Citrus sinensis) cultivated on road sides in Uyo Metropolis in Akwa Ibom State, Nigeria, International Journal of Ecological Science and Environmental Engineering. 4(6) (2017).

[53] K. Nolan, Copper Toxicity Syndrome, The Journal of Orthomolecular Psychiatry. 12 (2003) 270-282.

[54] S. Ravichandran, Possible natural ways to eliminate toxic heavy metals, International Journal of Chem. Tech. Research. 3(4) (2011) 1886-1890.

[55] J.O. Duruibe, J.N. Ewurugwu, M.O. Ogwuegbu, Heavy metal pollution and human biotoxic effects, International Journal of Physical Science. 2 (2007) 112-118.

[56] A. Alejandro-Córdova et al., Responses of arbuscular mycorrhizal fungi and grass Leersia hexandra swartz exposed to soil with crude oil, Water Air Soil Pollut. 228 (2017) 65.


[57] Y. Gao et al., Arbuscular mycorrhizal phytoremediation of soils contaminated with phenanthrene and pyrene, Journal of Hazardous Materials. 185 (2011) 703-709.


[58] D. Debiane et al., Lipid content disturbance in the arbuscular mycorrhizal, Glomus irregulare grown in monoxenic conditions under PAHs pollution, Fungal Biology. 115 (2011) 782-792.


[59] A. Volante et al., Influence of three species of arbuscular mycorrhizal fungi on the persistence of aromatic hydrocarbons in contaminated substrates, Mycorrhiza. 16 (2005) 43-50.


[60] C.N. Brady, R.R. Weil, The nature and properties of soil, New Jersey: Pearson Prentice Hall, (2008).

[61] L. Ke et al., Fate of polycyclic aromatic hydrocarbon (PAH) contamination in a mangrove swamp in Hong Kong following an oil spill, Marine Pollution Bulletin. 45 (2002) 339-347.

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