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Influence of Salt Stress on Proline and Glycine Betaine Accumulation in Tomato (Solanum lycopersicum L.)

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Abstract:

Many plants accumulate high levels of free proline content (pro) and glycine betaine (GB) in response to abiotic stress, Pro and GB act as an osmoprotectant. Generally, these levels are high than those required to be used in protein synthesis. Salinity inhibition of plant growth is the result of osmotic and ionic effect and different plant species have developed different mechanisms to cope with those effects. In this study, accumulation of osmolytes of twenty tomato genotypes was evaluated in response to salinity stress. The seedlings of each genotype were divided into three groups, Sodium chloride (NaCl) dissolved in irrigation water to make variant concentration of 30 and 60 mg/L of salt concentration using electrical conductivity meter which were used to water the plants. Level of free proline and glycine betaine were measured. Data obtained were subjected to one way analysis of variance using SPSS (20) Statistical Software. Dry mass accumulation decreased with increased salt concentration in all the genotypes. However, the result differ significantly (P< 0.05). The highest dry mass accumulations at control were recorded on Tropimech and Giofranco F. with 6.00 and 5.97. The lowest dry mass accumulations were recorded on plant treated with 60mg/L of salt. Dangainakawa recorded the least accumulation of dry mass on plants treated with 60mg/l of salt with 0.90g followed by Dan Gombe with 1.47g respectively. The highest free proline content of 1.46 µmolg-1 was recorded on Dan gainakawa at plant treated with 60 mg/L of NaCl. The lowest proline content was recorded at control on Giofranco F. with 0.17 µmolg-1 The highest GB content in all the plants were recorded at plants treated with 60 mg/L. However, the highest GB content (1.67) among the 20 (P<0.05) were recorded at 60 mg/L in Rio Grande followed by Bahaushe with 1.50 µmolg-1. In conclusion, GB and Pro are osmoregulators produced by tomato in response to stress so as to alleviate the consequence effects of salt stress.

Info:

Periodical:
Journal of Horticulture and Plant Research (Volume 1)
Pages:
19-25
Citation:
J.'afar Umar et al., "Influence of Salt Stress on Proline and Glycine Betaine Accumulation in Tomato (Solanum lycopersicum L.)", Journal of Horticulture and Plant Research, Vol. 1, pp. 19-25, 2018
Online since:
March 2018
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[1] M. Jamil, E.S. Rha, The effect of Salinity (NaCl) on the germination and seedling of sugar beet (Beta vulgari L.) and cabbage (Brassica oleraceae L.), Korean J. Plant Res. 7 (2006) 226-232.

[2] L.Y. Lira, S. Li, M. Showalter, Immunolocalization of extensin and potato tuber lectin in carrot, tomato and potato, Physiogia Plantarum. 97(4) (1996) 708-718.

[3] R. Munns, Comparative physiology of salt and water stress, Plant Cell Environ. 25 (2002) 239-250.

[4] J.K. Zhu, Plant salt tolerance. Trends in Plant Science. 6 (2001) 66-71.

[5] W. Sairam, S.A. Tygyi, Effects of soil salinity on germination and crop yield, Journal of Environmental Science. (2004) 43-49.

[6] A. Dudly, Growth and yield of vegetable, Crop Science. 21 (1992) 891-900.

[7] D. Prat, R.A. Fathi- Ettai, Variation in organic and mineral component in young Eukalyptus seedling under saline stress, Physiologia Plantarum. 79(3) (2013) 479-498.

[8] T.L. Shininger, The control of vascular development, Annual Review of Plant Physiology. 30 (1997) 313-337.

[9] H.J. Bohnert, D.E. Nelson, R.G. Jensen, Adaption of environmental stresses, Plant and Cell. 7 (1995) 1099-1111.

[10] H.J. Bohnert, R.G. Jensen, Strategies for engineering water stress tolerance in plants, Trend in Biotechnology. 14 (1996) 89-97.

[11] B. Brevitz, Organic solute content, R.C. Staples (Ed.), New York, (2004).

[12] A. Arthur, A Passion for Tomatoes, Smithsonian. 39(5) (2008) 54-62.

[13] T. Wada et al., Effect of foliar application of calcium solutions on the incidence of blossom-end rot of tomato fruit, Journal of the Japanese Society for Horticultural Science 65 (1996) 553–558.

[14] P. Carillo et al., Nitrogen metabolism in durum wheat under salinity: accumulation of proline and glycine betaine, Functional Plant Biology. 35 (2008) 412–426.

[15] IPGRI. Laboratory Bench activity glossary. How to calculate Leaf Surface area, 2014, pp.27-29.

[16] W. Jones, J. Gorham, E. MacDonnell, Organic and inorganic solutre content as selection crereria for salt tolerance in Triteceae, in: Salinity tolerance in plants, R.C. Staples, H. Gary, H. Toenniessen (Eds.), Wiley, New York, 1984, pp.189-203.

[17] F.T. Blum, Interpreting the metabolic responses of plants to water stress, Hort. Science. 15 (1996) 6223-629.

[18] F. Amini et al., Protern pattern changes in tomato under In vitro salt stress, Russian Journal of Plant Physiology. 54 (2007) 464-471.

[19] A.M. Gummi, A.A. Aliero, A study on cytotoxic ions sequestration and sodium/potassium levels as salt tolerant indicators in tomato, Journal of Biological Sciences. 4 (2012) 47-53.

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