In Vitro Propagation of a Vietnam Endemic Lady’s Slipper Orchid (Paphiopedilum vietnamense O.Gruss &amp; Perner)

Nguyen, Thi Tinh; Nguyen, Tien Dung; Dao, Xuan Thanh; Chu, Truc Dat; Ngo, Xuan Binh

doi:10.18052/www.scipress.com/JHPR.1.1

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In Vitro Propagation of a Vietnam Endemic Lady’s Slipper Orchid (Paphiopedilum vietnamense O.Gruss & Perner)

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Abstract:

P. vietnamense O. Gruss & Perner is one of the endemic lady’s slipper orchids in Vietnam that has been known as an endangered species threatened with extinction due to over-collection. To protect P. vietnamense species, In Vitro propagation method needs to be established for producing plantlets that can be introduced back in wild, as well as commercialized. In this study, we examined the effect of basal culture medium, plant growth regulators, and AC on the micropropagation ability of P. vietnamense, which were collected from wild population in Thai Nguyen province, Northeastern Vietnam. We determined that the 0.2% HgCl₂ can be used in within 5 to 10 min for sterilizing axillary buds of P. vietnamense, resulting in 65.55% of disinfected samples. Moreover, the 1/2 MS medium supplemented with 2.0 mg 1^-1BA and 1.0 mg 1^-1NAA, which gave about 2.85 shoots/sample, was the most suitable for shoot regeneration and multiplication. In addition, the result also indicated that 0.5 mg 1^-1NAA and 1.0 g 1^-1AC gave the best results (88.89%) for root induction and plantlets

Info:

Periodical:

Journal of Horticulture and Plant Research (Volume 1)

Pages:

1-8

DOI:

https://doi.org/10.18052/www.scipress.com/JHPR.1.1

Citation:

T. T. Nguyen et al., "In Vitro Propagation of a Vietnam Endemic Lady’s Slipper Orchid (Paphiopedilum vietnamense O.Gruss & Perner)", Journal of Horticulture and Plant Research, Vol. 1, pp. 1-8, 2018

Online since:

March 2018

Authors:

Thi Tinh Nguyen, Tien Dung Nguyen, Xuan Thanh Dao, Truc Dat Chu, Xuan Binh Ngo

Keywords:

BA, In Vitro, Propagation, Slipper Orchid

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Open Access

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Creative Commons Attribution 4.0 International License

References:

[1] S.J. Zeng et al. Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh, an endangered terrestrial orchid, Sci Hortic. 138 (2012) 198–209.

[2] S. Zeng et al., In vitro propagation of Paphiopedilum orchids, Crit Rev Biotechnol. 36 (2016) 521–534.

[3] Gruss, H. Perner. Paphiopedilum vietnamense A new species of Vietnam, Die Orchidee. 5 (1999) 1–8.

[4] H. Rankou, L. Averyanov, Paphiopedilum vietnamense, The IUCN Red List of Threatened Species, (2015).

[5] L.V. Averyanov et al., Slipper orchids of Vietnam. With an introduction to the flora of Vietnam, Kew Publishing, Royal Botanic Gardens, Kew, (2003).

[6] R. Ernst, Seed germination of Paphiopedilums, Orchid Rev. 88 (1982) 235–236.

[7] S.J. Zeng et al. Seed biology and in vitro seed germination of Cypripedium, Crit. Rev. Biotechnol. 35 (2015) 279–292.

[8] R.L.M. Pierik et al., Seed germination and further development of plantlets of Paphiopedilum ciliolare Pfitz in vitro, Sci. Hortic. 34 (1988) 139–153.

[9] T. Nagashima, Studies in the seed germination and embryogenesis in goeringii Rchb and Paphiopedilum insigne var. sanderae Rchb, Soc. Hortic Sci. 51 (1982) 94–105.

[10] S.J. Zeng et al., In vitro propagation of Paphiopedilum hangianum Perner & Gruss, Sci. Hortic. 151 (2013) 147–156.

[11] Z.L. Chen et al., Seed germination in vitro of Paphiopedilum armeniacum and P. Micranthum, Acta Hortic. Sin. 31 (2004) 540–542.

[12] T.Y. Chen, J.T. Chen, W.C. Chang, Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids, Plant Cell, Tissue and Organ Culture. 76(1) (2004) 11–15.

[13] T.Y. Chen, J.T. Chen, W.C. Chang, Multiple shoot formation and plant regeneration from stem nodal explants of Paphiopedilum orchids, In Vitro Cell Dev. Biol. Plant. 38 (2002) 595–597.

[14] A. Thongpukdee, E. Nisayan, C. Thepsithar, Multiple shoot formation of Paphiopedilum Delrosi,, World Acad. Sci. Eng. Techno. l (2013) 6–21.

[15] D.T. Nhut et al., In vitro stem elongation of Paphiopedilum delenatii Guillaumin and shoot regeneration via stem node culture, Propag. Ornam Plants. 7 (2007) 29–36.

[16] T. Murashige, F. Skoog, A revised medium for rapid growth and bio assays with tobacco tissue cultures, Physiologia Plantarum. 15 (1962) 473–497.

[17] D.B. Duncan, Multiple range and multiple F tests, Biometrics. 11 (1955) 1–42.

[18] J. Anburaj et al., Effects of plant growth regulators on callus induction from leaf explants of Cleome viscose, Res. J. Pharm. Biol. Chem. Sci. 2 (2011) 576.

[19] D. Preethi, T.M. Sridhar, C.V. Naidu, Efficient protocol for indirect shoot regeneration from leaf explants of Stevia rebaudiana (Bert.) – An important calorie free biosweetner, J. Phytol. 3(5) (2011) 56– 60.

[20] M. Firoz Alam et al., Studies on the effect of various sterilization procedure for in vitro seed germination and successful micropropagation of Cucumis sativus, Int. J. Pure App. Biosci. 4 (2016) 75-81.

[21] B. Sr Sagaya Mary, K.M. Divakar, In vitro propagation of Dendrobium jerdonianum Wight through flower stalk, leaf, nodes/ internodes as explants, American J. Life Scien. 4 (2016) 76-81.

[22] S.F. Bloomfield, The use of disinfectants in the home, J. Appl. Microbiol. 45(1) (1978) 1–38.

[23] M. Johnson et al., Antibacterial activity of leaves and inter nodal callus extracts of Mentha arvensis L, Asian Pacific J Trop Med. 4 (2011) 196–200.

[24] E.G. Wesely et al., Micropropagation of Alternanthera sessilis (L.) using shoot tip and nodal segments, Iranian J. Biotechnol. 9(3) (2011) 206–212.

[25] C.C. Ding, H. Wu, F.Y. Liu, Factors affecting the germination of Paphiopedilum armeniacum, Acta Bot. Yunnanica. 26 (2004) 673–677.

[26] N. Lee, Y.I. Lee, Effect of capsual maturity, medium composition and liquid culture on seed germination in vitro of Paphiopedilum spp, in: Taiwan Paphiopedilum Society, ed. Paphiopedilum in Taiwan II. Chiayi Hsien, Taiwan: Taiwan Paphiopedilum Society, 1999, p.10.

[27] Y. Zhou et al., Aseptic germination and rapid propagation of Paphiopedilum micranthum Tang, Guizhou Sci. 31 (2013) 79–82.

[28] B. Long et al., In vitro propagation of four threatened Paphiopedilum species (Orchidaceae), Plant Cell Tissue Organ Cult. 101 (2010) 151–62.

[29] K.P. Martin et al., In vitro propagation of Dendrobium hybrids using flower stalk node explants, Indian J Exp Biol. 43 (2005) 280-285.

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