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Neonauclea formicaria (Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos

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Abstract:

Plants are reservoirs of bioactive compounds with the potential for pharmaceutical use. In this study, the secondary metabolites of Neonauclea formicaria leaf crude ethanolic extract were determined using phytochemical screening. The plant's leaf extract was then used to test its angiogenesis activity using the chorioallantoic membrane (CAM) assay. Four concentrations of the extract were prepared—0.1 mg/L, 1.0 mg/L, 10.0 mg/L, and 100.0 mg/L and were topically applied on the CAM. Phytochemical screening revealed that N. formicaria leaves contain heavy amounts of flavonoids and tannins, while alkaloids, saponins, and steroids were present in trace amounts. The crude ethanolic extract was anti-angiogenic, as indicated by the significant decrease of vascular density at higher concentrations (P<0.05).  The 100 mg/L extract concentration showed the highest vascular inhibition (50.93%) among the other concentrations, suggesting its angiopreventive potential (P<0.05). Further investigation on the embryo's gross morphometry revealed no significant effects in the weight, crown-rump length, head-beak length, forelimb length, and hind limb length. Also, these indices were not associated with the angiogenesis activity on the CAM. Further studies exploring the specific metabolites of the different plant parts of N. formicaria and the plant's angiopreventive potential are recommended.

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Periodical:
International Letters of Natural Sciences (Volume 83)
Pages:
22-31
Citation:
J. Vergara et al., "Neonauclea formicaria (Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos", International Letters of Natural Sciences, Vol. 83, pp. 22-31, 2021
Online since:
July 2021
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[1] A.M. Joy, M.R. Appayoo, M.G. Mohesh, Anti-angiogenic Activity of Strychnos nux vomica Leaf Extract on Chick Chorioallantoic Membrane Model, Journal of Chemical and Pharmaceutical Research. 8 (2016) 549–552.

[2] C. Ngelangel, E. Wang, Cancer and the Philippines Cancer Control Program, Japanese Journal of Clinical Oncology. 32 (2010) 52–61.

[3] N. Nishida et al., Angiogenesis in Cancer, Vascular Health Risk Management. 2 (2006) 213–219.

[4] J. Folkman, Tumor Angiogenesis Therapeutic Implications, New England Journal of Medicine.285 (2006) 1182–1186.

[5] W.T. Fajardo et al., Phytochemical Analysis and Anti-Angiogenic Potential of Gmelina arborea roxb. (Paper Tree) Fruit Exocarp using Duck Chorioallantoic Membrane (CAM) Assay, Asia Pacific Journal of Multidisciplinary Research. 3 (2015) 72-79.

[6] S.M. Samudra, Ethnomedicinally Important Plants of Family Rubiaceae from Pune District (MS), In Proceeding of National Conference on Environment and Development. 2016, pp.86-88.

[7] S.C. Kala, Medicinal Attributes of Family Rubiaceae, International Journal of Pharmacy and Biological Science. 5 (2015) 179-181.

[8] C.P. Diaz, A.S. Jose, Current Practices on Medicinal Plants in the Philippines. Canopy, 26 (2000) 5.

[9] Y.K. Tang, M.A. Rahman, A. Zajmi, In vitro Anti-oxidant and Anti-bacterial Activities of Leaf and Branch Extracts of Morinsindica L, Journal of Management and Science. 16 (2018) 8-13.

[10] N.P. Peteros, Studies on the Structure and Bioactive Components of Selected Philippine Medicinal Plants (Brucia amarissima l., Intsia bijuga (c)o.k., Laportea meyenniana l., and Pipturus arborescens w.) PhD dissertation, Graduate School, MSU-Iligan institute of Technology. (2010).

[11] J.A.D. Ordas, C.I. Banag, G.H.D. Alejandro, Neonauclea viridiflora (Rubiaceae), a new species of Naucleeae from Eastern Samar, with notes on myrmecophytic species in the Philippines, Systematic Botany. 42 (2017), 364-370.

DOI: https://doi.org/10.1600/036364417x695592

[12] M.T. Demetillo, G.L. Betco, A.B. Goloran, Assessment of native medicinal plants in selected mining area of claver Surigao Del Norte, Philippines. Journal of Medicinal Plants. 7 (2019), 171-174.

[13] N. Karaket et al., Chemical and Bioactivity Evaluation of the Bark of Neonauclea purpurea, Natural Product Communications. 7 (2012) 169-170.

[14] S.K. Bussa, P. Jyothi, Antidiabetic Activity of Stem Bark of Neolamarckia cadamba in Alloxan induced Diabetic Rats. International Journal of Pharmacy and Technology. 2 (2010) 314-324.

[15] S.T. Khan et al., Anti-diabetic Potential of Aerial Parts of Galium tricornutum (Dandy) Rubiaceae, Tropical Journal of Pharmaceutical Research. 16 (2017) 1573-1578.

DOI: https://doi.org/10.4314/tjpr.v16i7.15

[16] A.A. Aiyeloja, O.A. Bello, Ethnobotanical Potentials of Common Herbs in Nigeria: A Case Study of Enugu State, Educational Research and Reviews. 1 (2006), 16.

[17] M.A. Abbasi et al., Evaluation of Comparative Antioxidant Potential of Aqueous and Organic Fractions of Ipomoea carnea, Journal of Medicinal Plants Research. 4 (2010) 1883-1887.

[18] M. Li et al., The in ovo chick chorioallantoic membrane (CAM) assay as an efficient xenograft model of hepatocellular carcinoma, JoVE (Journal of Visualized Experiments). 2015, e52411.

DOI: https://doi.org/10.3791/52411

[19] C.S. Kue et al., Chick embryo chorioallantoic membrane (CAM): an alternative predictive model in acute toxicological studies for anti-cancer drugs, Experimental animals. (2015) 14-0059.

DOI: https://doi.org/10.1538/expanim.14-0059

[20] J. Rosal et al., Effects of Prenatal Exposure to Urea Fertilizer on the Angiogenesis, Body Growth, and Liver Structure of Duck (Anas platyrhynchos) Embryos, Pollution. 7 (2021), 367-375.

[21] A.M. Aguinaldo et al., A Guidebook to Plant Screening: Phytochemical and Biological, University of Santo Tomas Publishing House, Espana, Manila, (2005).

[22] D. Ribatti, The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM Assay in the Study of Angiogenesis and Metastasis, Springer Science & Business Media, (2010).

DOI: https://doi.org/10.1007/978-90-481-3845-6_3

[23] F.J. Rohlf, tpsDig version 2.12, Ecology and Evolution, SUNY at Stony Brook, (2008).

[24] F.J. Rohlf, Tps Utility Program version 1.44, Ecology and Evolution, SUNY at Stony Brook, (2009).

[25] J.P.M. Gamallo et al., Evaluation of Anti-angiogenic Property of Ocimum basilica Ethanolic Leaf Extract by using Duck Embryo Chorioallantoic Membrane (CAM) Assay and its Morphometric Analysis, International Journal of Herbal Medicine. 4 (2016) 22-26.

[26] H. Tuba et al., The In vivo Evaluation of Anti-angiogenic Effects of Hypericum Essential Oils using the Chorioallantoic Membrane Assay, Pharmaceutical Biology. 52 (2014) 44-50.

DOI: https://doi.org/10.3109/13880209.2013.810647

[27] Y.J. Yuan et al., Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease, International Journal of Medical Sciences. 11 (2014) 1275-1281.

DOI: https://doi.org/10.7150/ijms.10443

[28] A.N. Panche, A.D. Diwanz, S.R. Chandra, Flavonoids: An Overview, Journal of Nutritional Science. 5 (2016) 1275.

[29] A. Ortuno et al., Citrusparadisi and Citrus sinensis Flavonoids: Their Influence in the Defence Mechanism against Penicillium digitatum, Food Chemistry. 98 (2006) 351–358.

DOI: https://doi.org/10.1016/j.foodchem.2005.06.017

[30] H.A. Nouvlessounon et al., Phytochemical Analysis and Biological Activities of Cola nitida Bark, Biochemistry Research International. 13 (2015) 358-375.

[31] E. Sieniawska, T. Baj, Tannins, In Pharmacognosy, Academic Press, 2015, pp.199-232.

[32] A.G. Cagauan, M.C. Galaites, L.J. Fajardo, Evaluation of Botanical Piscicides on Nile Tilapia Oreochromis niloticus L. and Mosquito Fish Gambusia affinis Baird and Girard, In Proceedings on ISTA, 2004, pp.179-187.

[33] J.H. Doughari, Antimicrobial activity of Tamarindus indica Linn. Tropical Journal of Pharmaceutical Research. 5 (2006), 597-603.

[34] M. Wink, Modes of Action of Herbal Medicines and Plant Secondary Metabolites, Medicines. 2 (2015) 251-286.

DOI: https://doi.org/10.3390/medicines2030251

[35] T. Schmeller, A. El-Shazly, M. Wink, M. Allelochemical Activities of Pyrrolizidine Alkaloids: Interactions with Neuroreceptors and Acetylcholine Related Enzymes. Journal of Chemical Ecology. 23 (1997) 399-416.

DOI: https://doi.org/10.1023/b:joec.0000006367.51215.88

[36] O. Schimmer, M. Wink, Molecular Modes of Action of Defensive Secondary Metabolites, In Functions and Biotechnology of Plant Secondary Metabolites. Annual Plant Reviews, 2018, pp.21-161.

DOI: https://doi.org/10.1002/9781119312994.apr0418

[37] M. Moller et al., The Alkaloid Emetime as a Promising Agent for the Induction and Enhancement of Drug-induced Apoptosis in Leukemia Cells, Oncology Reports. 18 (2007) 737-744.

[38] O. Güçlü-Üstündağ, G. Mazza, Saponins: Properties, Applications and Processing, Critical Reviews in Food Science and Nutrition. 47 (2007), 231-258.

DOI: https://doi.org/10.1080/10408390600698197

[39] A.Y. Bagrov, J.I. Shapiro, O.V. Federova, Endogenous Cardiotonic Steroids: Physiology, Pharmacology and Novel Therapeutic Targets, Pharmacological Reviews. 61 (2009) 9-38.

DOI: https://doi.org/10.1124/pr.108.000711

[40] S.H. Goh et al., A Phytochemical Study of Borneo: Selected Plants from Sabah Lowland Forests, Journal of Herbs, Spices & Medicinal Plants. 5 (1997), 29-52.

DOI: https://doi.org/10.1300/j044v05n01_05

[41] A. Itoh et al., Two Chromone-Secoiridoid Glycosides and Three Indole Alkaloid Glycosides from Neonauclea sessilifolia, Phytochemistry. 62 (2003), 359-369.

DOI: https://doi.org/10.1016/s0031-9422(02)00541-1

[42] H. Tosa et al., Anthraquinones from Neonauclea calycina and their Inhibitory Activity Against DNA Topoisomerase II, Biological and Pharmaceutical Medicine. 21 (1998) 641–642.

DOI: https://doi.org/10.1248/bpb.21.641

[43] F.P. Chang et al. Three new iridoid derivatives have been isolated from the stems of Neonauclea reticulata (Havil.) Merr. with cytotoxic activity on hepatocellular carcinoma cells, Molecules. 23 (2018), 2297.

DOI: https://doi.org/10.3390/molecules23092297

[44] F.P. Chang et al, Four New Iridoid Metabolites Have Been Isolated from the Stems of Neonauclea reticulata (Havil.) Merr. with Anti-Inflammatory Activities on LPS-Induced RAW264.7 Cells, Molecules. 24 (2019), 4271.

DOI: https://doi.org/10.3390/molecules24234271

[45] F.P. Chang et al, Four New Iridoids isolated from the stem of Neonauclea reticulate, Planta Medica International Open. 4 (2017), Mo-PO.

[46] A.M. Iannuzzi et al., Antiangiogenic Iridoids from Stachys ocymastrum and Premna resinosa, Planta Med. 85 (2019), 1034-1039.

DOI: https://doi.org/10.1055/a-0889-0412

[47] K.A. Beladjila et al., Antiangiogenic Activity of Compounds Isolated from Anarrhinum pedatum. Journal of natural products. 82 (2019), 510-519.

DOI: https://doi.org/10.1021/acs.jnatprod.8b00893

[48] C. Lou et al., Picroside II, an iridoid glycoside from Picrorhiza kurroa, suppresses tumor migration, invasion, and angiogenesis in vitro and in vivo. Biomedicine & Pharmacotherapy. 120 (2019), 109494.

DOI: https://doi.org/10.1016/j.biopha.2019.109494

[49] S.J. Jeong et al., Antiangiogenic Phytochemicals and Medicinal Herbs, Phytotherapy Research. 25 (2011) 1-10.

[50] O. Kadioglu, E.J. Seo, T. Efferth, Targeting Angiogenesis by Phytochemicals. Med Aromat Plants. 2 (2013) 1-8.

DOI: https://doi.org/10.4172/2167-0412.1000134

[51] S.C. Gupta et al., Upsides and Downsides of Reactive Oxygen Species for Cancer: The Roles of Reactive Oxygen Species in Tumorigenesis, Prevention, and Therapy, Antioxidants & Redox Signaling. 16 (2012), 1295-1322.

DOI: https://doi.org/10.1089/ars.2011.4414

[52] R. Gaziano et al., Antitumor Effects of the Benzophenanthridine Alkaloid Sanguinarine: Evidence and Perspectives, World Journal of Gastrointestinal Oncology. 8 (2016) 30.

DOI: https://doi.org/10.4251/wjgo.v8.i1.30

[53] J. Chen et al., The flavonoid Nobiletin Inhibits Tumor Growth and Angiogenesis of Ovarian Cancers via the Akt Pathway, International Journal of Oncology. 46 (2016), 2629-2638.

DOI: https://doi.org/10.3892/ijo.2015.2946

[54] J.P. Eun, G.Y. Koh, Suppression of Angiogenesis by the Plant Alkaloid, Sanguinarine, Biochemical and Biophysical Research Communications. 317 (2004), 618-624.

DOI: https://doi.org/10.1016/j.bbrc.2004.03.077

[55] A. Vargas et al., The Chick Embryo and Its Chorioallantoic Membrane (CAM) for the In vivo Evaluation Of Drug Delivery Systems, Advanced Drug Delivery Reviews. 59 (2007) 1162-1176.

DOI: https://doi.org/10.1016/j.addr.2007.04.019

[56] J. Baharara et al., Anti-angiogenesis Effect of Biogenic Silver Nanoparticles Synthesized using Saliva officinalis on Chick Chorioallantoic Membrane (CAM), Molecules. 19 (2014), 13498-13508.

DOI: https://doi.org/10.3390/molecules190913498

[51] J. Guo et al., In ovo Exposure to Triclosan Alters the Hepatic Proteome in Chicken Embryos. Ecotoxicology and Environmental Safety. 165 (2018) 495-504.

DOI: https://doi.org/10.1016/j.ecoenv.2018.09.043

[52] P.F. Surai, V.I. Fisinin, F. Karadas, Anti-oxidant Systems in Chick Embryo Development, Part 1. Vitamin E, Carotenoids and Selenium. Animal Nutrition. 2 (2016) 1-11.

DOI: https://doi.org/10.1016/j.aninu.2016.01.001
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