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Phytochemical Properties of Roselle (Hibiscus sabdariffa, L.) Plants Grown under Bio and Mineral Fertilizers in Different Types of Soil

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The aim of this study was to clarify the phytochemical property evaluation of Roselle plants grown under bio Azotobacterine (Azotobacter chroococcum) and phosphorein (Bacillus polymyxa) and mineral (N, P and K fertilizers at the rates of 25, 50 and 100% from the doses recommended by Ministry of Agriculture) fertilizers applied in different types of soil. Samples of Roselle plants were obtained from different soils (clay soil at Dar El-Ramad farm, sandy loam and saline loamy sand soil at Demo farm, Faculty of Agriculture) at El-Fayoum governorate conditions. Moreover, some of the phytochemical properties (N, P and K percentage in roselle herb and its uptake, photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), anthocyanin pigment and pH value) of roselle plants (Hibiscus sabdariffa, L.) under different soils were determined. The data obtained showed that, bio and mineral (NPK) fertilizers increased the above compositions of roselle plants under different soils of experiment. The maximum increase of these compositions was obtained by the treatment clay soil × 100% NPK + bio fertilizers, followed by clay soil × 50% NPK + bio fertilizers as compared to saline loamy sand soil × non fertilizer treatment, although, the differences between these treatments and mineral fertilizer at the rate of 100% NPK alone were insignificantly. Therefore, it is economically and environmentally recommended to inoculate roselle seeds with mixture of Azotobacter + Bacillus and fertilize these inoculated plants with 50% NPK for improve chemical compositions (N, P and K percentage in roselle herb and its uptake, photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), anthocyanin pigment and pH value) of roselle plants under clay soil. Key words: Roselle, Hibiscus sabdariffa L., nitrogen, phosphorus, potassium, biofertilization, soil type, salinity, chemical composition.


International Letters of Natural Sciences (Volume 83)
S. S.I. Ghabour et al., "Phytochemical Properties of Roselle (Hibiscus sabdariffa, L.) Plants Grown under Bio and Mineral Fertilizers in Different Types of Soil", International Letters of Natural Sciences, Vol. 83, pp. 1-14, 2021
Online since:
July 2021

[1] Mahadevan, N., R. Shivali, P. Kamboj (2009). Hibiscus subdariffa Linn. - An overview. Nat. Prod. Rad., 8(1): 77-83.

[2] Copley, L.S. (1975). An introduction to the botany of tropical crops. Longman Group, U.K.

[3] Ahmed, A.H.R. and A.M. Nour (1981). Promising Karkade seed derivatives: Edible oil and karkade, Annual Report, Food Research Center. Shambat, Sudan.

[4] Tewari, S. and N.K.n Arora (2016). Fluorescent Pseudomonas sp. PF17 as an efcient plant growth regulator and biocontrol agent for sunflower crop under saline conditions. Symbiosis 68: 99–108.


[5] Arora, N.K., M. Verma, J. Prakash, J. Mishra (2016). Regulation of biopesticides: global concerns and policies. In: Bioformulations for sustainable agriculture. Springer, New Delhi, p.283–299.


[6] Shaheen A.M., A.n Rizk Fatma , M. Omiama Sawan, A.A. Ghoname (2007). The integrated use of Bio-inoculants and chemical nitrogen fertilizer on growth, yield and nutritive value of two okra (Abelmoschus Esculentus, L.).cultivars. Australian Journal of Basic and Applied Sciences, 1(3): 307-312.

[7] Ahmad, M. Ahmad, I., T.H. Hilger, S.M. Nadeem, M.F. Akhtar, M. Jamil, A. Hussain, Z.A. Zahir (2018). Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions. Peer J 6:e5122.


[8] Segura, L.G.C. and K.M.R., Sialongo (2005). Bio-N fertilizer application on the growth and yield of sweet corn (Zea mays var.rogu sa). A special project presented to the faculty of crop science unit college of agriculture, xavier university in partial fulfillment of the requirement for the course Agri. 124.Online.

[9] Khalil, M.A. I., A. A. M. Mohsen, M.K. Abdel-Fattah (2016).Effect of Bio and Mineral Nitrogen Fertilization on Growth, Yield and Quality of Lettuce Plants under Sandy Soil Conditions. Middle East Journal of Applied Sciences 6(2):411-417.

[10] Abdel-Nabi, H. M. A., K. D. Kawther, E. I. El-Gamily, Y. F. E. Imryed (2016). Impact of mineral, organic and bio fertilization on growth, yield and quality of cantaloupe. J. Plant Production, Mansoura Unv. (11): 1777-1794.


[11] Gawali, R., R. Shila, P.K. Unni (2018). Integrated Nutrient Management Approach on Wheat (Triticum aestivum L.) in Vertisols Int. J. Curr. Microbiol. App. Sci. 7(4): 3144-3153.


[12] Singh, D. Y.V. and S. Tyagi (2019). Effect of Biofertilizer, Herbicide Application and Nitrogen Management on Growth, Productivity of Wheat (Triticum aestivum L.). Int.J.Curr.Microbiol. App.Sci (2019) 8(4): 2712-2719.


[13] Hammad, K. H. A. and H. A. Hussein (2016). Effect of NPK and chicken manure on the growth productivity and som growth components of squash (Cucurbita pepo L.). ARPN J. of Agric. And Bio sci. 11(6): 230-235.

[14] El-Araby, S. M., I. M. Ghoneim, A. I. Shehata, R. A. Mohamed (2003). Effect of nitrogen, organic manure and biofertilizer applications on strawberry plants. 1- Vegetative growth, flowering and chemical constituents of leaves. J. Agric. & Env. Sci. Alex. Univ. Egypt. 2 (2): 36-62.

[15] Martínez, J.M., J.A. Galantini, M.E. Duval, (2018). Contribution of nitrogen mineralization indices, labile organic matter and soil properties in predicting nitrogen mineralization. J. Soil Sci. Plant Nutr. 18: 73–89.


[16] Aranguren, M., A.N Castellón, A.n Aizpurua (2018). Topdressing nitrogen recommendation in wheat after applying organic manures: The use of field diagnostic tools. Nutr. Cycl. Agroecosyst. 110: 89–103.


[17] Siddiqui, M.H., F. Chandoad, M.K. Abbasi, A.W. Yogandahi (2009).Effect of NPK, micronutrients and n-placement on the growth and yield of sunflower. Sarhad J. Agric., 25(1): 45-52.

[18] Khan, A.A., G. Jilani, M.S. Akhtar, S.M. Naqvi, M. Rasheed (2009). Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J. Agric. Biol. Sci. 1 (1):48-58.

[19] El-Ganaini, S.S. (2009). Effect of phosphorus and some micronutrients fertilizers on growth, yield and some chemical constituents of sunflower (Helianthus annuus L.) plants grown under reclaimed soil conditions. Fayoum J. Agric. Res. & Dev., 23 (2):94-107.

[20] El-Nagdy, G.A., D. M. Nassar, E.A. El-Kady, G. S. El-Yamanee (2010). Response of flax plant (Linum usitatissimum L.) to treatments with mineral and bio-fertilizers from nitrogen and phosphorus. Journal of American Science, 6(10):207-217.

[21] Yosefi1, K., M. Galavi1, M. Ramrodi, S.R. Mousavi (2011). Effect of bio-phosphate and chemical phosphorus fertilizer accompanied with micronutrient foliar application on growth, yield and yield components of maize (Single Cross 704). AJCS,5(2):175-180.


[22] Ragab, T. (2016). Magesium and phosphorien application improve the efficiency of growth and productivity of squash plants grown on sandy calcareous soil. J. of Advanced Botany and zoology (4): 1-6.

[23] Baliah,T. N.and A.S. Priya (2017).Effect of bioformulations of Phosphate Solubilizing Bacteria (PSB) on the Growth and Biochemical Characters of the Gossypium Hirsutum and Zea Mays. International Journal of Environment, Agriculture and Biotechnology (IJEAB), 2 (6): 3229-3236.


[24] Zheng, B.X., M. Ibrahim, D.P. Zhang, Q.F. Bi, H.Z. Li, G.W. Zhou, K. Ding, J. Peñuelas, Y.G. Zhu, X.R. Yang (2018). Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method. AMB, Expr. 8(47): 2-12.


[25] Li, H., X. Ding, C. Chen, X. Zheng, H. Han, C. Li, J. Gong, T. Xu, Q. X. Li, G. C. Ding, J. Li, (2019).Enrichment of phosphate solubilizing bacteria during late developmental stages of eggplant (Solanum melongena L.). FEMS Microbiology Ecology 95(3)pii: fiz023.


[26] Prakash, J. and N. Arora (2019). Phosphate-solubilizing Bacillus sp. enhances growth, phosphorus uptake and oil yield of Mentha arvensis L. 3 Biotech 9(126):1-9. 10.1007/s13205-019-1660-5.


[27] Allen, O. N. (1971). Experimentals in Soil Bacteriology. Burgess Publishing, Minneapolis, Minnesota, USA.

[28] Parkinson, J. A. and S. E. Allen (1975) A wet oxidation process suitable for the determination of nitrogen and mineral nutrients in biological material.

[29] Jackson, M. L. (1973). Soil Chemical Analysis. Prentice- Hall., Inc. Englewood Claiffs, New Jersey, USA.

[30] Page, A. I., R. H. Miller, D. R. Keeney (Eds). (1982). Methods of Soil Analysis, part- 2. Chemical and Microbiological Properties. 2nd (Ed). American Society of Agronomy, Madison, Wisconsin. USA.

[31] Hafez, A. R. and O. S. Mikkelsen (1981). Colorimetric determination of nitrogen for evaluating the nutritional status of rice. Communications Soil Science and Plant Analysis, 12 (1): 61-69.


[32] Diab, M. A. (1968). The chemical composition of Hibiscus sabdariffa L. M. Sc. Thesis, Fac. Agric., Cairo Univ.

[33] Fahmy, R. (1970). Different Quantitative Estimations of Some Organic Compounds in Plants, pp.72-73 (in Arabic), Anglo Egypt. Press, Cairo, Egypt.

[34] Cherry, J. H. (1973). Molecular Biology of Plants (A text manual). Columbia Univ. Press. New York.

[35] Snedecor, W. C. and W. G. Cochran (1980). Statistical Methods. 7th ed. The Iowa state Univ. Press, Ames, Iowa USA.

[36] Moawad, H. M. H. (2005). Effect of phosphorus and potassium fertilization on growth, yield and resistance of wheat plant to pathogens under Fayoum governorate conditions. M. Sc. Thesis, Fac. Agric. Fayoum Univ., Egypt.

[37] El-Saidi, M. T. and M. Hawash (1971). Effect of using saline water for irrigation on the growth and chemical properties of roselle plants (Hibiscus sabdariffa, L.). Z. Acher Und Pflanzen bau, 134: 251-256.

[38] Strogonov, B. P. (1962). Physiological basis of salt tolerance of plants. Academy of Special Science of USSR, Instituted of Plant Physiology. English Translation by Israel Program for Scientific Translation, 2964.

[39] Lapina, L. P. (1966). Effect of high iso-aromatic compounds on nitrogen and carbohydrate metabolism in maize. Sov. Plant Physiol. 13, 1029.

[40] Parida, A. K. and A. B. Das (2005). Salt tolerance and salinity effects on plants: a review, Ecotoxicol. Environ. Safety 60, 324–349.

[41] Sharpley, A. N., J. J. Meisinger, J. F. Power, D. L. Suarez (1992). Root extraction of nutrients associated with long-term soil management, advances springer- Verlag, 151-217.


[42] Damke, M. M. and S. K. Bhattacharjee (1997). Influence of NPK fertilization on flower yield and seasonal in leaf nutrient content of rose cv. Super Star. PKV research J. 21 (1): 39-43. (C. F. Hort. Abst. 68 (4): 3387).

[43] Lambers, H., F. S. Chapin, T. L. Pons (2000). Plant Physiological Ecology. Spring –Verleg, New York. Inc.

[44] Abou El-Seoud, M. A., M. F. Abd El-Sabour, E. A. Orner (1997). Productivity of roselle plant (Hibiscus sabdariffa, L.) as affected by organic waste composts addition to sandy soil Bull. Natl. Res. Center, Cairo, Egypt, 22: 495-505.

[45] Pandey, S. N. and B. K. Sinha (1978). Plant Physiology 2nd revised ed., p.362.

[46] Hartmann, A. (1989). Ecological aspects of growth and nitrogen fixation with of Azospirillum (C.F. Nitrogen fixation with Non-Legumes by Shiner et al.,123-136, Kluwer Acad. Publ., Dordrecht, Netherlands.


[47] Hegde, D. M., B. S. Dwivedi, B. S. S. Sudhakara (1999). Biofertilizers for cereal production in Indian. A review. Indian J. Agric. Res., 69 (2): 73-83.

[48] Bashan, Y. and H. Levanony (1990). Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture. Can. J. microbial, 36: 591-608.


[49] Follett, R. H., L. S. Murphy, R. L. Dorahue (1981). Fertilization and Soil Amendments. Pretice-Hall. Inc. Inglewood Cliffs, New Jersey, 07632, USA.

[50] Kenawy, A. G. M. (2005). Effect of some biofertilization treatments on the growth, yield and chemical composition of Hibiscus sabdariffa L. plants. M. Sc. Thesis, Fac. of Agric. Minia Univ.

[51] Subba Rao, N. S. (1984). Biofertilizers in Agriculture Oxford. IBH Company. New Delhi.

[52] Gaudry, J. F. M. (2001). Nitrogen assimilation by plants: physiological, bacterial and molecular aspects. Published by science publishers, Inc. Enfield, NH, USA, pp.185-197.

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