Amino Acids Composition of Liver, Heart and Kidneys of Thryonomys swingerianus (Temminck 1827) Compared

: Amino acids composition of Thryonomys swingerianus is reported. Whereas protein values (g100g -1 ) had liver (74.1), kidney (91.5), heart (84.6); corresponding total amino acid values were 93.5, 83.2 and 80.6. True protein from the crude protein of the samples ran thus: liver>kidney>heart. Of the twenty parameters reported on, liver was best in 12/20 (60.0%), kidney and heart both shared the second position of 4/20(20%) each. Among the essential amino acids, leucine predominated in both liver (7.96g100g -1 protein) and kidney (8.11g100g -1 protein) but valine (6.21g100g -1 protein) predominated in the heart. The P-PER values were; P-PER 1 : 2.78 (liver), 2.91(kidney), 0.716 (heart) and P-PER 2 : 2.71 (liver), 2.90 (kidney), 0.564 (heart). However, there was a reverse between liver and kidney in the EAAI values with liver (92.0) > kidney (90.2) > heart (87.6) with corresponding BV values of 88.5 > 86.6 > 83.7. In the amino acids scoring pattern, Ser was limiting in liver (0.533) and heart (0.394) but Thr (0.490) in kidney in whole hen’s egg score comparison; in FAO/WHO scoring standards, Thr was limiting in liver (0.988) and kidney (0.625) but Leu (0.459) in heart. In pre-school requirements, liver recorded no limiting amino acid whereas Thr was limiting in kidney (0.735) and Leu was limiting in the heart (0.486). T. swingerianus red viscera was compared with the red viscera of livestock animals (cattle, sheep and pork) as well as FAO/WHO/UNU standards for total essential amino acids. Our results when compared with the livestock red viscera (without Trp) and FAO/WHO/UNU (g100g -1 protein), we have heart: grasscutter/cattle/sheep/pig: 45.3 /46.0/42.7/46.6; kidney: grasscutter/cattle/sheep/pig: 47.6/43.8/42.5/46.7; liver: grasscutter/cattle/ sheep/pig: 50.7/47.7/41.5/47.5 and grasscutter liver/kidney/heart/ FAO/WHO/UNU: 50.7/47.6/45.3/32.8 showing that all the red viscera values in T. swingerianus were better than the essential amino acids in the FAO/WHO/UNU standards and livestock red viscera. Statistical values showed that significant differences existed among the samples at r =0.01. PubChem is a database of chemical molecules and their activities against biological assays. Each compound in PubChem is identified by a unique system Compound Identifier (CID) accession number. The system is maintained by the National Center for Biotechnology Information (NCBI), a component of the National Library of Medicine, which is part of United States National Institute of Health (NIH). PubChem contains substance descriptions and small molecules with fewer than 1000 atoms and 1000 bonds [1]. Precisely, CID is Compound Identification Number [2].


Introduction
Grasslands are sources of food, forage and energy as well as providing a natural habitat for a variety of wildlife that provide a bulk of the food resources for 800 million of the world's population [3]. According to Stypinski [4], grasslands provide forage for grazing and browsing animals, both domestic and wild; African grasslands wildlife include the giant rodent called the grasscutter (by West Anglophone Africa), hedgehog (Central Africa), agouti (in French-speaking West Africa), greater cane rat (Eastern and Southern Africa).
Wildlife has been an important protein source for many West African countries over centuries [8]. Rodents are known to be the most preferred and commonly consumed bush animals and remain as the main source of protein for developing countries [9,10]. Rural dwellers depend mostly on bush meat as an economic and protein source for livelihood [11]. Ghana and Nigeria are well-known for their consumption of bush meat. Bush meat is popular and important in many traditional festivities among African communities [12] and among the wild animals is the grasscutter [5]. Grasscutter farming is common nowadays and it is farmed as a micro-livestock species in many African countries [13]. In Southern Nigeria, grasscutter is one of the most sold and consumed bushmeat [14] since we have no religious prohibitions for the consumption of grasscutter [15,16]. Crude protein content of grasscutter is about 22.7% compared with 20.7% for rabbit, 19.25% for chicken and 18.2% for beef with less fat and cholesterol than beef, mutton and pork [17]. Adeyeye et al. [18] reported on the lipid profile of the skin, muscle and liver of grasscutter and found that all of them contained good PUFA, showed PUFA/SFA value was higher than the minimum recommended value of 0.45; with values of 0.86 (skin), 1.26 (muscle) and 1.07 (liver). Also, Adeyeye and Jegede [19] noted that high levels of both essential and non-essential amino acids are in the meat of grasscutter. In many developing countries meat animals are frequently slaughtered only for the carcass, whereas a number of byproducts, which can be obtained quite easily, could help to improve the supply of low cost, high protein foods for people [20]. Meat animals yield, apart from their carcasses, a considerable amount of parts which are biologically and hygienically fit for human consumption. They are generally consumed either as main ingredients in traditional dishes or as ingredients in meat products. Heart, kidney and liver are all consumed as direct meat products. The so-called red viscera: liver, heart, kidneys, tongue and neck sweetbread (thymus) are normally obtained and marketed as "fancy meats" [20].
Heart is an organ which is in almost all instances an edible part of an animal. Among the various sorts of offal, it is unusual in that it consists almost entirely of muscle. Hearts of older animals are most likely to be tough and to need marinating before being cooked [21]. Liver is a relatively large organ in most animals, birds and fish, usually edible and in some cases delicious. Livers are appreciated in most parts of the world although consumption is low in North America. Livers lend themselves well to the making of pastes, stuffing, sausages and the like. Among the other ingredients which go well with animal livers are bacon, outmeal, sour cream, sharp apples, onions, shallots,

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garlic, marjoram and sage [21]. Kidney is rich in nutrients and proteins. Kidney meat contains omega 3 fatty acids. It is also known to contain anti-inflammatory properties and to be good for the heart. Further, a number of benefits have been ascribed to kidney beans. It is well known that a person's health, physical development and work capacity depend on the fulfilment of protein requirements both in quantity and in quality. Protein quality is evaluated from the content of essential amino acids, the relative proportions in which they are present and the extent to which the protein is digested [20]. This study wants to report on the amino acids composition of three fancy meats (liver, heart, kidney) and to find out if any significant difference exists in the amino acid compositions of the three red viscera organs of Thryonomys swinderianus.

Materials and Methods
Collection of samples: Local hunters were commissioned for the supply of grasscutter caught in the wild from Iworoko Ekiti, Nigeria. The animals (five of them) were authenticated in the Department of Wildlife, Fisheries and Environment, Ekiti State University, Ado Ekiti, Nigeria.

Sample treatment:
The method used in processing the grasscutter meat was by scalding. Scalding involved dipping the animal in hot water to ease the removal of the fur [8]. The animal fur was removed and the animal dissected. The three fancy meats were carefully removed, washed in distilled water and oven dried to constant weight. This was dry milled into flour, kept in the laboratory refrigerator at 37 0 F (2.8 0 C), pending analyses.

Determination of amino acids
Defatting: Weights taken ranged from 0.1366 -0.1688 g of the samples into extraction thimble, fat extracted with chloroform/methanol mixture using a Soxhlet extraction apparatus [22]. The extraction lasted 5-6 h.
Hydrolysis of the samples: Between 30 to 35 mg of the defatted samples were weighed into glass ampoules. Seven millilitres of 6 M HCl were added and oxygen was expelled by passing nitrogen gas into the ampoule (to avoid possible oxidation of some amino acids during hydrolysis). Each glass ampoule was then sealed with a Bunsen flame and put into an oven at 105 o C ±5 O C for 22h.
The ampoule was then allowed to cool before breaking open at the tip and the content was filtered to remove the humins.
The filtrate was then evaporated to dryness at 40 o C under vacuum in a rotary evaporator. Each residue was then dissolved with 5 ml of acetate buffer and stored in a plastic specimen bottle and kept in the deep freezer.

Sample analysis:
The method of analysis was by ion exchange chromatography (IEC) [23]. The amount loaded for each sample was 5 -10 µl each. These were dispensed into the cartridge of the analyzer. The Technicon Sequential Multisample Amino Acid Analyzer (TSM) (Technicon Instruments Corporation, New York) was used for the analysis. The TSM analyzer was designed to separate and analyse free acidic, neutral and basic acids of the hydrolysate. The period of an analysis lasted for 76 min for each sample. The column flow rate was 0.50 mL/min at 60 o C with reproducibility consistent within ±3 %. The net height of each peak produced by the chart record of the TSM (each representing an amino acid) was measured and calculated. The values reported were averages of two determinations. Tryptophan was not determined due to cost. The nitrogen was estimated by the micro-Kjeldahl method [24] with crude protein taken as N x 6.25. Norleucine (PubChem, CID 21236) which is an unnatural amino acid is usually used experimentally to study protein structure and function. It was used as the internal standard in the analyses for the amino acids composition.
Some calculations were made from the analytical results: i.

Estimation of isoelectric point(pI):
The estimation of the isoelectric point (pI) for a mixture of amino acids could be carried out using the equation of the form [25]:

International Letters of Natural Sciences Vol. 79 25
(1) where IPm was the isoelectric point of the mixture of amino acids, IPi was the isoelectric point of the i th amino acid in the mixture and Xi was the mass or mole fraction of the i th amino acid in the mixture.

Estimation of predicted protein efficiency ratio (P-PER):
Computation of protein efficiency ratio (C-PER or P-PER) was done using the equations suggested by Alsmeyer et al. [26]. ii.
Leucine/isoleucine ratio: The leucine/isoleucine ratios, their differences and their percentage differences were calculated. iii.
Estimation of essential amino acid index (EAAI): The method of EAAI calculation due to Oser [27] using the egg protein amino acid as the standard.

Computation of amino acid scores:
The amino acid scores were computed using three different methods:  Scores based on amino acid values compared with whole hen's egg amino acid profile [28].  Scores based on essential amino acid scoring pattern [29].  Scores based on essential amino acid suggested pattern of requirements for preschool children [30].

Statistical evaluation
Data results from relevant Tables were subjected to statistical analysis of correlation coefficient (rxy), coefficient of determination (rxy 2 ), regression (Rxy), coefficient of alienation (CA) and index of forecasting efficiency (IFE). Other calculations made were the grand mean, standard deviation (SD) and coefficient of variation (CV%). The rxy was converted to the critical table value to see if significant differences existed among the three fancy meats amino acid results at r=0.01 [31].

Results
In Table 1 we have the amino acid profiles for the three samples. On parameter basis, the values variation was low since the coefficient of variation (CV%) ranged between 2.58 and 43.4. The most varied amino acid was Leu whose values ranged between 3.21-8.11g100g -1 and the least varied was Ile with values of 4.02-4.21 g100g -1 protein. The Chi-square (χ 2 ) values on parameter basis were all not significant at α= 0.05 since all χ 2 c < χ 2 T. Among the amino acids, the most concentrated value was observed in Glu with values of 12.0-13.1g100g -1 , mean (12.7) and CV% (4.58); it is an acidic amino acid. The least concentrated amino acid was Cys ranging from 1.502.20g100g -1 , mean (1.95) and CV% (20.0). Among the essential amino acids, Leu was the most concentrated and closely followed by Lys with values of 5.61-6.66g100g -1 protein, ±6.26 and CV% (9.04); least essential amino acid was Thr with values of 2.50-3.95g100g -1 , mean (2.98) and CV% (28.1). Whereas the highest total crude protein was in kidney (91.5g100g -1 ) > heart (84.6g100g -1 protein) > liver (74.1g100g -1 protein); the total amino acid trend was liver (93.5g100g -1 protein) > kidney (83.2g100g -1 protein) > heart (80.6g100g -1 protein). This showed that liver moved from the third position (crude protein, 74.1g100g -1 ) to first position in the amino acid profile (93.5g100g -1 ); kidney was in the first position in the crude protein (91.5g100g -1 ) but in the second position in the amino acid profile (83.2g100g -1 ); the heart was in the second position in crude protein (84.6g100g -1 ) but in the third position in the amino acid profile (80.6g100g -1 ). The amino acid composition showed that the true protein trend in the samples was liver > kidney > heart.
The amino acid scores in comparison with whole hen's egg amino acid profile have been shown in Table 4. All the χ 2 values were low and not significant at α=0.05. Also, the CV% were low at 2.58 -38.0. The scores in Gly, Glu and Phe were each either 1.00 or >1.00. Other amino acids with scores >1.00 were Pro (liver, heart); Lys (liver, kidney); Arg (liver, heart) and Cys (liver, heart). The limiting amino acid varied between Ser and Thr. In Ser, it was 0.533 (liver) and 0.394 (heart) whereas Thr (0.490) was limiting in kidney.
Data in Table 4 was statistically analyzed as shown in Table 5. All the three rxy were high and significant at levels of 0.7818 -0.8413; although values were lower than in Table 2. The rxy 2 values were also high (0.6113 -0.8413) but lower than in Table 2. The range of Rxy was 0.5565 -1.04, again lower than in Table 2. The CV% values were low at 37.0 -45.2. The CA values were much higher than in Table 2 with values of 0.5406 -0.6235 with much lower IFE (compared with Table 2) values at 0.3765 -0.4594. That is, CA > IFE, hence, prediction of relationship between paired samples would be difficult since the reduction in the error of prediction was low.
In Table 6 we have the score comparison between our amino acids results and the FAO/WHO [29] standards. Whereas Thr was limiting in liver (0.988) and kidney (0.625), Leu (0.459) was limiting in the heart. All the χ 2 values were not significantly different at α= 0.05. Also, all the CV% values were low and ranged from 2.31 -28.1. In the liver, only Thr had its score ˂1.0; in the kidney, both Val and Thr had their scores < 1.0 and in the heart, Thr and Leu had their score values < 1.0.
The data from Table 6 were statistically analyzed and the results were shown in Table 7. The rxy values were low (0.4705 -0.6576) and not significant at r = 0.01. The mean, SD and CV% were low. The CA values were high with corresponding low values of IFE having values of 11.76% -24. 66%.
The pre-school essential amino acid scores were shown in Table 8. No limiting amino acid values were observed in the liver; all values were > 1.00. In the kidney only Thr had <1.00 score hence it was the limiting amino acid in the kidney. In the heart, Thr (0.735), Leu (0.486) and Lys (0.967) had scores < 1.00 each but Leu was the limiting amino acid. All χ 2 values were not significant.
The CV% values ranged from 2. 42 -43.5. It is interesting to note that Leu had CV% of 43.4 in Table  6 (FAO/WHO comparison) and Leu also recorded CV% of 43.5 in Table 8 (pre-school child requirements comparison).
In Table 9, data from Table 8 were statistically analyzed. The rxy value for liver/kidney was low (0.6269) and insignificant at r= 0.01. On the other hand, rxy values for kidney/heart (0.8621) and liver/heart (0.8072) were both high and significant at r = 0.01. The rxy 2 values were low to high at 0.3930 -0.7431. Rxy was much better than the values in Table 7 with values of 0.9627 -2.21. Values of mean, SD and CV% were all generally low. CA was high (0.4391 -0.7791) whereas IFE was low (0.2209 -0.5609). Figure 1 contained the summary of the amino acids profiles into Factors A and B. The importance of this was to see if values in column for factor A means would be similar to Factor B means. Figure 2 depicted the red viscera of grasscutter compared to the livestock animals (cattle, sheep and pig) viscera and the FAO/WHO/UNU [30] standard.

Discussion
The overall amino acid profiles in Table 1 showed varied distributions of each type of amino acids in each sample. These parameters were generally high in the samples: Lys, His, Arg, Pro, Gly, Cys, Val, Met, Ile, Leu, protein and total amino acids. The trend of concentration for high levels of amino acids in liver was: Glu > Asp > Gly > Lys = Arg > Val > Phe > Pro; in kidney concentration ran thus: Glu > Leu > Asp > Lys > Arg > Gly > Phe; in heart we have: Glu > Gly > Val > Asp > Lys > Phe. Hence while liver had nine parameters having values greater than 5.00g100g -1 protein, kidney had seven and heart had six. Whereas liver had four EAA in its first topmost nine parameters, both kidney and heart had three each. The values observed for the crude protein and total amino acids were of major interest. Liver provided the lowest protein value (74.1g100g -1 ) but recorded the highest value of total amino acid (93.5g100g -1 protein). The most probable reason must be that the liver had more true protein than both other samples. So, the run for the true protein in the samples would be liver>kidney> heart. The protein values in the red viscera of Cricetomys gambianus were (g100g -1 ): heart (74.8), kidney (85.8) and liver (83.9) [32]; these values were also highly comparable with the present results. In the organs of guinea -fowl, the protein values (g100g -1 ) were: liver (73.6) and heart (74.9) [33]. The amino acid profiles of the present report compared very favourably with the amino acid profiles of the heart and liver of Cricetomys gambianus [34]. However, the amino acid values in African giant pouch rat liver was 86.9g100g -1 and 86.3g100g -1 (heart) [34].
The class and group divisions of the amino acids were depicted in Table 3. Class I group were amino acids with aliphatic side chains (hydrogen and carbons) = Gly, Val, Leu, Ile. The percentage values range here ran as 29.7-32.0 with CV% of 4.58. Class II were amino acids with side chains containing hydroxylic (OH) groups = Ser, Thr having total percentage values of 6.96 -8.73 and CV% (11.3). Class III were the (TSAA) of Met and Cys, these were amino acids with side chains containing sulphur atoms. Their percentage range was 4. 45  The TSAA of the samples ranged between 4.16-6.76 g100g -1 which were highly comparable or even higher to the value of 58mgg -1 protein recommended for infants [30]. The aromatic amino acid range suggested for ideal protein (68-118mgg -1 protein) [30] was highly comparable with present report with values in the total essential aromatic amino acid (10.5 -11.1g100g -1 protein). The present values were almost equivalent to the ideal protein and hence could very well serve as a food supplement to low protein food sources. Values of the EAA were 40.5 -45.1g/100g with percentage values of 48.2 -52.0. These values were above the 39% considered adequate for ideal protein food for infants, 26% for children and 11% for adults [30]. The EAA/TAA in egg is 50% [35]. In the predicted protein efficiency ratios, we had these values: P-PER1 (0.716 -2.91) and P -PER2 (0.564 -2.90). The in vivo P-PER is of the order of 2.2 [36]. In Cricetomys gambianus, the P-PER for liver was 2. 62 and the one for heart was 2.32 [34]. From these results, it was obvious that the heart will be the least physiologically utilized protein among the samples. Among the various sorts of offal, the heart is unusual in that it consists almost entirely of muscle. Moreover, the nature of the organ is such that the muscles are in constant use, pumping blood around the body, while the animal is alive; and hearts of older animals are therefore likely to be tough and to need marinating before being cooked [21].
It is well known that a person's health, physical development and work capacity depend on the fulfilment of protein requirements both in quantity and in quality. Protein quality is evaluated from the content of essential amino acids, the relative proportions in which they are present and the extent to which the protein is digested [20]. In general, it has been found that the better the protein, the lower the level in the diet required to produce the highest protein efficiency ratio. This is a clear reflection of the importance of the proper nutritive balance of the amino acids to produce optimum metabolic efficiency. The essential amino acid index (EAAI) of 87.6 -92.0 and their corresponding biological values (BV) of 83.7 -88.5 depicted the high quality of the protein of the samples. From literature, we have the following EAAI and BV values for comparison [27]: milk, cow (whole, non-fat, evaporated or dry), EAAI (88) and BV (84, predicted; 90, observed); human, EAAI (87) and BV (83); eggs, chicken (whole, raw or dried), EAAI (100), BV (97, predicted; 96, observed); whites (raw or dried), EAAI (95), BV (92, predicted; 93, observed); yolks (raw or dried), EAAI (93), BV (89, predicted); shellfish (shrimp, including prawns, raw or canned), EAAI (67), BV(61, predicted); Neopetrolisthes maculatus (another shellfish), EAAI (86.9 -89.9) and BV (83.0 -86.3) [37]. Using another method of EAAI determination [38] in Cricetomys gambianus, we have values of EAAI 1.31 (liver) and 1.20 (heart) [34]. These literature values show the quality position of the three fancy meats. EAAI is useful as a rapid tool in the evaluation of food formulation for protein quality.
Further to evaluate the protein quality of the fancy meats, Leu/Ile values were calculated getting ratio values of 0.762 -2.02. The most ideal Leu/Ile value is 2.36 [23]. It had been suggested that an amino acid imbalance from excess Leu might be a factor in the development of pellagra [39]. A high Leu imbalance in the diet impairs the metabolism of Trp and niacin, and is responsible for the niacin deficiency in sorghum eaters [40]. Experiments in dogs showed that animals fed sorghum proteins with less than 11g100g -1 protein Leu did not suffer from nicotinic acid deficiency [41]. The present Leu values were 3.21 -8.11g100g -1 protein, they were therefore considered safe and could be beneficially exploited to prevent pellagra in endemic areas [42]. The values of Leu/Ile ratio of 0.762 -2.02 showed that we might not experience concentration antagonism in the samples when consumed as protein source in food. The percentage Cys /TSAA values were 28.3 -51.7. The (Cys/TSAA)% in kidney (28.3) and heart (32.5) depicted the usual protein observation for animals shown as follows  [37]. However, the value of 51.7% in the liver showed a behaviour of a plant %Cys/TSAA; see these literature examples: 62.9% in coconut endosperm [44]; its range was 58.9 -72.0% in guinea corn [45]; it was 50.5% in cashew nut [46]; it was 40.7% in Triticum durum [47] and 44.4% in Parkia biglobosa [48]. The percentage Cys in TSAA in the diet of rat, chick and pig is 50% [23] but the value is unknown in man [30]. The presence of cystine and cysteine in the diet would reduce the needs for Met and since almost all the sulphur in the diet was derived from these three amino acids the sulphur content might sometimes be used as an approximate assessment of the adequacy of protein [49]. The isoelectric point, pI, was 4.80 -5.51 showing the samples to be in the acidic medium of the pH range. The pI calculation from amino acids usually assists in the quick production of certain isolate of organic product without evaluating the protein solubility to get to the pI.
In Table 4 where we have the amino acids in comparison with whole hen's egg, Ser was limiting in liver (0.533), Thr in kidney (0.490) and in the heart (0.490). Therefore, in order to fulfill the day's needs for all the amino acids in the three fancy meats, 100/53.3 or 1.88 times as much liver protein, or 100/49.0 or 2.04 times as much kidney protein and heart protein, would have to be eaten when each serve as the sole protein source in the diet. The data in Table 4 were translated statistically as shown in Table 5. All the rxy values were positively high and significant at r = 0.01. Prediction of relationship was slightly difficult because CA> IFE in each compared sample parameters. The Rxy was only greater than 1.00 in kidney/heart (1.04) but others were lower than 1.00g100g -1 protein.
In Table 6, we have the amino acid scores as compared with FAO/WHO [29] standards. Thr was limiting in liver (0.988) and in the kidney (0.625) but Leu (0.459) was limiting in the heart. In the hiearachy of limiting amino acids, Lys is the first limiting amino acid, second is Met + Cys, third is Thr and fourth is Trp. However, for the basis of discussion, Leu was reckoned with here. Hence, correction factors for liver would be 100/98.9 or 1.01; for kidney correction would be 100/45.9 or 2.18; for Leu correction was 100/45.9 or 2.18. In the statistical result shown in Table 7, all the rxy values were low and none was significant at r= 0.01; CA˃˃IFE but two of Rxy values were each greater than 1.00g100g -1 protein.
The essential amino acid scores comparison with pre-school child (2-5y) requirements were shown in Table 8. Liver values were all better than the standard values since scores recorded were between 1.12-1.83. In kidney, Thr was limiting at 0.735 with correction factor of 100/73.5 or 1. 36 and Leu (0.486) was limiting in heart and has a correction factor of 100/48.6 or 2.06. Values in Table  9 resulted from the statistical analysis of the values in Table 8. The rxy values were positively low to high with those of kidney/heart and liver/ heart being significantly different among each pair. The highest recorded value of Rxy was observed in liver/ heart with a value of 2.21g100g -1 protein. The CA > IFE in liver/kidney and kidney/heart but the reverse was observed in liver/heart. The summary of the amino acid profiles into Factors A and B was shown in Figure 1. Factor A means constituted amino acids values of the three samples along the vertical axis; Factor B means constituted the amino acid values along the vertical axis as shown in the figure; both containing the essential and non-essential amino acids. Column under Factor B means showed close values at a close range of 42.80 -42.96g100g -1 protein. On the whole, the mean of Factor A means and factor B means gave a value of 42.88g100g -1 protein. Figure 2 contained information on the comparison of the grasscutter viscera (liver, heart, kidney) with the livestock animal (cattle, sheep, pig) viscera and FAO/WHO/UNU [30] standards. The essential amino acid content of almost all the organs in the grasscutter was very similar to that of viscera from all the three animals and good enough to maintain the nitrogen balance in the organism.

Conclusions
Thryonomys swingerianus liver, kidney and heart samples were all good sources of highquality protein of more than average requirements of essential amino acids, high P-PER, high EAAI, high BV but low Leu/Ile ratios. The WHO recommended Val and Ile requirements for school children aged 10 -12 years of 33 and 30 mg amino acid kg -1 body weight day -1 [29,30]. For example, a 30kg child will require 990 and 900 mg of Val and Ile day -1 respectively. From Table 1, 100g of liver protein would provide 4742mg Val, kidney would provide 4172mg Val and heart would provide 5254mg Val to 30kg child whereas liver would provide 3112mg Ile, kidney would provide 3678 mg Ile and heart would provide 3562 mg Ile to 30 kg child. Whereas the fancy meats would provide more than 4x (four times) the Ile requirements, the samples would provide more than 3x (three times) the Val requirements for school children.

Conflict of interest
Authors declare no conflict of interest  Significant diff. Mean1 SD1 and CV%1, all represented the corresponding values in the first member of a pair whereas 2, 2, 2, series corresponded to the second pair of the group; *rxy was significant at n-2 (df) = n-2= 18-2 =16 at r= 0.01 with critical value of 0.590   Table 2; CA and IFE, see Table 2; *Results significantly different at r=0.01, n-2(df) = n-2 =18-2=16 at critical level of 0.590 For Mean1 SD1, CV%1 and the 2, 2, 2, series, see Table 2; CA and IFE, see Table 2. NS = results not significantly different at r = 0.01, n-2 = 8-2 = 6 (df), critical level of 0.834. values in the first member of a pair whereas 2, 2, 2, series corresponded to the second pair of the group; NS = result not significantly different at r = 0.01; *Results significantly different at r = 0.01, n-2(df) = n-2 = 9-2 = 7 at critical level of 0.798

International Letters of Natural Sciences Vol. 79
Cystine (Cys) and tyrosine (Tyr) are not essential, but have an economizing effect regarding methionine (Met) and phenylalanine (Phe), respectively.

References
[1] PubChem Source Information, The PubChem project. National Center for Biotechnology Information, USA.