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International Letters of Natural Sciences
ILNS Biostimulation and Overcoming the Abiotic Stresses in Plants

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Impact of Ascorbic Acid Foliar Spray and Seed Treatment with Cyanobacteria on Growth and Yield Component of Sunflower Plants under Saline Soil Conditions

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A field experiments were conducted during the two summer seasons of 2015 and 2016 in saline soil (ECe = 9.0 dSm-1), at Fayoum province, Egypt to study the effect of ascorbic (AsA), as foliar application alone or combined with cyanobacteria (CB) on growth, yield, its components and nutritional status of sunflower plants variety Sakha 54. Treatments comprised 2 ascorbic acid (AsA) levels with or without seed inoculation with cyanobacteria (CB). They were 1 mM AsA, 2 mM AsA, 1 mM AsA + CB and 2 mM AsA + CB in addition to the control treatment in which seeds were not received CB and their plants were sprayed with distilled water. Results could be summarized as follows: increasing the addition of ascorbic acid concentration up to 1Mm with combination of CB increased significantly values of growth attributes (e.i., plant height, of leaves no. /plant, shoot dry weight and leaf area), photosynthetic pigments (chlorophyll a, b and carotenoids), physiological responses (total soluble sugars, proline and soluble phenols) as well as the head diameter, seed yield/ plant, 100 seed weight and seed yield (t ha-1). Also, N, P, K, Fe, Mn, Zn and oil percentage of sunflower seeds with compared to control treatment in both years. Generally, the results in most cases, demonstrate that the all parameters increased significantly by using the concentration of ascorbic acid 1Mm combined with CB in both seasons


International Letters of Natural Sciences (Volume 76)
A. N. A.A. Abdel-Hafeez et al., "Impact of Ascorbic Acid Foliar Spray and Seed Treatment with Cyanobacteria on Growth and Yield Component of Sunflower Plants under Saline Soil Conditions", International Letters of Natural Sciences, Vol. 76, pp. 136-146, 2019
Online since:
August 2019

[1] Akladious SA, Hanafy RS (2018). Alleviation of oxidative effects of salt stress in white lupine (LUPINUS TERMIS L.) plants by foliar treatment with l- arginine. The J. Anim. Plant Sci. 28(1):(2018).

[2] FAO (2009). Food and Agriculture Organization of United Nations, Agric Data FAO.

[3] Manai JT, Kalai H, Gouia, Corpas FJ (2014). Exogenous nitric oxide (NO) ameliorates salinity-induced oxidate stress in tomato (Solanum lycopersicum) plants. J Soil Sci and Plant Nutr 14(2): 433-446.


[4] Hussein MM, AbouLeila BH, Abdel-Hady, NF (2009). Influence of putrecine on growth of barly (Hordeum Vulgarel) grown under salinity. Egypt J.Agron, 31(2): 301-309.

[5] Abdel-Monem AA, El-Bassiouny HMS, Rady MM, Gaballah, MS (2010). The role of tryptophan and prozac (S-hydroxy tryptophan) on the growth, some biochemical aspects and yield of two sunflower cultivars grown in saline Soil. International Journal of Academic Research, 2(4): 231-225.

[6] Saffan, SE (2008). Effect of salinity and osmotic stress on some economic plants. Research Journal of Agriculture and Biological Science, 4(2): 159-166.

[7] Semida WM, Abd El-Mageed TA, Howladar SM, Rady MM (2016). Foliar-applied α-tocopherol enhances salt-tolerance in onion plants by improving antioxidant defense system. Aust. J. Crop Sci. 10(7), 1835–2707.


[8] Conklin, P (2001). Recent advances in the role and biosynthesis of ascorbic acid in plants. Plant Cell Environment, 24: 83-94.

[9] Shalata A, Neumann PM (2001). Exogenous ascorbic acid (vitamin C). Increases resistance to salt stress and reduces lipid peroxidation. J. Exp. Bot., 52: 2207 – 2211.


[10] FAS-USDA (2017). Foreign Agricultural Service/United States Department of Agricultural. May (2017).

[11] Rai, A (2015). Salt Tolerance by cyanobacteria and reclamation of USAR soil. Indian Journal of Plant Sciences ISSN: 2319–3824.

[12] Kaushik, BD, Subhashini D (1985). Amelioration of salt affected soils with blue green algae II. Improvement in soil properties. Proceedings of Indian National Science Academy B51 386-389.

[13] Jackson, ML (1967). Soil Chemical Analysis,. New Delhi, Prentice Hall of India Private Limited, New Delhi, p.144–197 and 326–338.

[14] A.O.A.C., 1995. Official Methods of Analysis of the Association of Official Agricultural Chemists. Sixteenth ed., Washington D.C., USA.

[15] Arnon, DI (1949). Copper enzymes in isolated chloroplast. Polyphenol-oxidase in Beta vulgaris L. Plant Physiol. 24, 1–5.

[16] Snell FD, Snell CT (1953). Colorimetric methods of analysis including some turbimetric and morpholometric methods. D. Van- Nastrod Co., Inc. New Jersey, Toronto, New York, London, Vol. III, P. 606.

[17] Irigoyen, J.J., D.W., Emerich, and M., Sanchez-Diaz, 1992. Water stress induced changes in the concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant. 8, 455–460.


[18] Bates LS, Waldeen RP, Teare, ID (1973). Rapid determination of free proline for water stress studies. Plant Soil. 39, 205–207.


[19] Mukherjee SP, Choudhuri MA (1983). Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol. Plant. 58, 166–170.


[20] Hafez AR., Mikkelsen DS (1981). Colorimetric determination of nitrogen for evaluating the nutritional status of rice. Commun. Soil Sci. and Plant Anal., 12 (1), 61–69.

[21] Piper CS (1947). Soil and Plant Analysis,. The University of Adelaide, Adelaide, UK.

[22] Page AI, Miller RH, Keeny DR (1982). Methods of Soil Analysis,. Part II. Chemical and Microbiological Methods. 2nd Ed. Amer. Soc. Agron., Madison, Wisconsin, USA.

[23] Chapman HD, Pratt PF (1961). Methods of Analysis for Soil,. Plants and Water, Univ. Calif., D.V., Agric. Sci., USA.

[24] Lowry OH, Rosebrough NJ, Farr AL (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193(1), 265–275.

[25] Kono, Y (1978). Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch. Biochem. Biophys. 186 (1), 189–195.


[26] Aebi H (1984). Catalase in vitro. Methods Enzymol. 105, 121–126.

[27] Putter, J (1974). Peroxidase. In: Bergmeyer, H.U. (Ed.), Methods of Enzymatic Analysis. VerlagChemie, Weinhan, p.685–690.

[28] Snedecor WC, Cochran WG (1980). Statistical Methods. 7th ed. The Iowa State Univ. Press, Ames, Iowa, USA.

[29] El-Kobisy DS, Kady KA, Medani RA, Agamy RA (2005). Response of pea plant Pisum sativum L. to treatment with ascorbic acid,. Egypt. J. Appl. Sci., 20: 36-50.

[30] Athar H, Khan A, Ashraf M (2008). Exogenously applied ascorbic acid alleviates salt induced oxidative stress in wheat,. Environ. Exp. Bot.63: 224-231.


[31] Azza AMM, Sahar MZ, Safaa AM, Hanan SS (2011). Stimulatory effect of kinetin, ascorbic acid and glutamic acid on growth and chemical constituents of Codiaeum variegatum L. plant,.American-Eurasian J. Agric. And Environ. Sci.10: 318-323.

[32] Rady MM, Hemida KhA (2016). Sequenced application of ascorbate-proline-glutathione improves salt tolerance in maize seedlings. Ecotoxicol. Environ. Saf. 133,252–259.


[33] Rady MM, Sahar S, Taha, Sebnem K (2018). Integrative application of cyanobacteria and antioxidants improves common bean performance under saline conditions. Scientia Horticulturae 233, 61–69.


[34] Abd El-Mageed TA, Semida WM, Mohamed GF, Rady MM (2016). Combined effect of foliar-applied salicylic acid and deficit irrigation on physiological–anatomical responses, and yield of squash plants under saline soil. S. Afr. J. Bot. 106, 8–16.


[35] Habibi, G (2012). Exogenous salicylic acid alleviates oxidative damage of barley plants under drought stress. Acta Bio. Szeged 56, 57–63.

[36] Rodriguez AA, Stella AA, Storni MM, Zulpa G, Zaccaro MC (2006). Effects of cyanobacterial extracellular products and gibberellic acid on salinity tolerance in Oryza sativa L. Saline System. 2: 7-15.


[37] Mengel K, Kirkby E (2001). Principles of plant nutrition. 5th ed. Dordrecht: Kluwer Academic Publishers.

[38] Damon PM, Rengel Z (2007). Wheat genotypes differ in potassium efficiency under glasshouse and field conditions. Aust J Agric Res. 58:816–823.


[39] Saadatnia H, Riahi H (2009). Cyanobacteria from paddy fields in Iran as a biofertilizer in rice plants. Plant Soil and Environ. 55: 207 – 212.


[40] Singh PK, Prakash J, Singh SK, Shukla M (2008). Cyanophycean algae inhabiting sodic soil exhibit diverse morphology: An adaptation to high exchangeable sodium. Ecoprint. 15: 15-21.


[41] Talaat NB (2003). Physiological studies on the effect of salinity, ascorbic acid and putrescine of sweet pepper plant. Ph.D. Thesis, Fac. Agric.Cairo Univ., Egypt.

[42] Amin AA, El-Sh M, Rashadand FAE Gharib. (2008). Changes in morphological, physiological and reproductive characters of wheat plants as affected by foliar application with salicylic acid and ascorbic acid'. Aust. J. Basic and appl. Sci. 2: 252-261.

[43] Ashraf, MPJC, Harris, PJC (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166 (1), 3–16.


[44] Karthikeyan N, Prasanna R, Sood A, Jaiswal P, Nayak S, Kaushik BD (2009). Physiological characterization and electron microscopic investigations of cyanobacteria associated with wheat rhizosphere. Folia Microbiol. 54, 43–51.


[45] Dixit V, Pandey V, Shyam R (2001). Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad). J. Exp. Bot. 52 (358), 1101–1109.


[46] Mittova, V, Tal M, Volokit, Guy M (2002). Salt stress induces up-regulation of an efficient chloroplast antioxidant system in the salt-tolerant wild tomato species Lycopersicon pennellii but not in the cultivated species. Physiol. Plant 115 (3).


[47] Pastori GM, Kiddle G, Antoniw J, Bernard S, Veljovic-Jovanovic S, Verrier PJ, Noctor G, Foyer CH (2003). Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. Plant Cell, 15: 939–951.


[48] Convertini G, Ferri D, Montemurro F, Maiorana M (2004). Effects of municipal solid waste compost on soils cropped with tomato and sunflower in rotation with durum wheat. Proc. of the 13th International Soil Conservation Organization Conference, 628: 1-4.

[49] Maiorana M, Charfeddine M, Montemurro F, Vonella AV (2005). Reduction of agronomic inputs in sunflower (Helianthus annuus L.). HELIA, 28, Nr. 42, 133-146.


[50] Louisa MA, Taguiling G (2013). Quality improvement of organic compost using green biomass. European Scientific Journal, 9: 319-341.

[51] Fatemi SN (2014). Ascorbic acid and its effects on alleviation of salt stress in sunflower. Annual Research & Review in Biology 4:3656-3665.

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