Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > ILNS Volume 72 > Reaction of Cells Desmodesmus armatus (Chod.)...
< Back to Volume

Reaction of Cells Desmodesmus armatus (Chod.) Hegew. on the Induction of Carotynogenesis

Full Text PDF


Various studies of the components of the antioxidant protection system of microalgae D. armatus under the influence of osmotic stress and active forms of oxygen will allow to develop methods for controlling carotenogenesis in a given culture and to obtain carotenoid enriched feed for zooplankton. These studies made it possible to evaluate the activity of catalase, peroxidase enzymes in cells that are cultured under the induction of carotenogenesis by free radical oxidation promoters and osmotic stress on the background of physiological changes. It is established that under these conditions, there is an increase in volumes and aggregation of vegetative cells. At the same time, the amount of biomass remains at the level of the first day of inductors application. Against the background of a decrease in growth activity, a decrease in the number of metabolically active cells in cytochrome oxidase was observed. It is also shown that, when iron sulfate is introduced with hydrogen peroxide and sodium chloride against the background of enhanced carotenogenesis, antioxidant systems are activated by increasing the activity of catalase and peroxidase. Under such conditions, it is possible to achieve increased production of carotenoids in Desmodesmus armatus culture.


International Letters of Natural Sciences (Volume 72)
L. Cheban et al., "Reaction of Cells Desmodesmus armatus (Chod.) Hegew. on the Induction of Carotynogenesis", International Letters of Natural Sciences, Vol. 72, pp. 21-27, 2018
Online since:
November 2018

[1] I. Bogut et al, Nutritional value of planktonic cladoceran Daphnia magna for common carp (Cyprinus carpio) fry feeding, Ribalstvo, 68 (2010) 2-12.

[2] T. John, Carotenoids: physical, chemical, and biological functions and properties, Edit. Landrum CRC Press, (2009) 568.

[3] M. Molinе at al., Production of torularhodin, torulene, and β-carotene by Rhodotorula yeasts, Methods Mol. Biol., 898 (2012) 275-283.


[4] W. Stahl Bioactivity and protective effects of natural carotenoids, Biochimica et Biophysica Acta, 1740 (2005), 101-107.


[5] A. Catarina Guedes, Nutritional value and uses of microalgae in aquaculture, Aquaculture, (2014) 59-78.

[6] G.S. Minyuk, E.S. Chelebieva, I.N. Chubchikova, Special features in the secondary carotenogenesis Bracteacoccus minor (Chlorophyta) in a two-stage culture, Algologia. 25 (2015) 21-34. (In Russian).


[7] Y. Lemoine, B. Schoefs, Secondary ketocarotenoid astaxanthin biosynthesis in algae: a multifunctional response to stress, Photosynth. Res. 106 (2010) 155-177.


[8] S. Takaichi, Carotenoids in Algae: Distributions, Biosyntheses and Functions, Mar. Drugs, 15 (2011) 110-118.

[9] N. Mallick, F.H. Mohn, Reactive oxygen species: response of algal cells, Journal of Plant Physiology. 157 (2000) 183-193.


[10] S. Boussiba, Carotenogenesis in the green alga Haematococcus pluvialis: cellular physiology and stress response, Physiol. plant. 108 (2000) 111-117.


[11] R.Y. Ma, F. Chen, Enhanced production of free trans-astaxanthin by oxidative stress in the cultures of the green microalga Chlorococcum sp., Proc. Biochem. 36 (2001) 1175-1179.


[12] H.H. El-Baky, F.K. El Baz, G.S. El-Baroty, Production of antioxidant by the green alga Dunaliella salina, Int. J. Agri. Biol. 6 (2004) 49-57.

[13] L.M. Cheban, I.V. Malishchuk Induction of the secondary carotenogenesis in Desmodesmus armatus (Chod.) Hegew under conditions of two-stage cultivation, Ukr. Biochem. J., 88 (2016) 106. (In Ukrainian).

[14] D.R. Aliyeva, H.G. Babayev, I.V. Azizov, Activity and isoform content of peroxidase in Dunaliella saline cells under salt stress. Visnyk of Dnipropetrovsk University. Biology. Medicine. 18 (2010) 16-21. (In Russian).


[15] D.R. Aliyeva, H.G. Babayev, I.V. Azizov, Effect of elevated NACL concentration to the photosynthesis and activity of catalase in Dunaliella salina cells, Visnyk of Dnipropetrovsk University. Biology. Ecology. 17 (2009) 3-9. (In Russian).


[16] J. Kato, et al, Characterization of catalase from green algae Chlamydomonas reinhardtii, Journal of Plant Physiology, 151(1997) 262-268.


[17] N.D. Tupik, E.K. Zolotareva, Isoensyme spectrum в1'яцof Chlorophyta peroxidase, Algologia. 18 (2008) 123-133. (In Russian).

[18] L.M. Cheban, I.V. Malischuk, M.M. Marchenko, Cultivating Desmodesmus armatus (Chod.) Hegew. in recirculating aquaculture systems (RAS) waste water, Arch. Pol. Fish. 23 (2015) 155-162.


[19] F.D Caprio, et al, Two stage process of microalgae cultivation for starch and carotenoid production, Chemical Engineering Transactions. 16 (2016) 415-420.

[20] R.H. Hevorhyz, S.H. Shchepachyov Metodyka yzmerenyia plotnosty suspenzyy nyzshykh fototrofov na dlyne volnы sveta 750 nm. – Sevastopol: Otdel byotekhnolohyy y fytoresursov YnBIuM NAN Ukraynu, 2008. (In Russian).

[21] O.V. Vasilenko, et al, Energy and nitrogen metabolism in Chlorella vulgaris Beij. (Chlorophyta) the influence of sodium gypsum, Algologia. 24 (2014) 297-301. (In Ukrainian).

[22] O.H. Lowry, et al, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.

[23] A.A. Tammam, E.M. Fakhry, M. El-Sheekh, Effect of salt stress on antioxidant system and the metabolism of the reactive oxygen species in Dunaliella salina and Dunaliella tertiolecta, African Journal of Biotechnology 10(19) (2011) 3795-3808.

[24] M. Zhang, et al, Effects of salt stress on ion content, antioxidant enzymes and protein profile in different tissues of Broussonetia papyrifera, South African Journal of Botany, 85 (2013) 1-9.


[25] A. Caverzan et al., Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection, Genet Mol Biol. 35 (2012) 1011-1019.


[26] A. Sofo, et al, Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses, Int. J. Mol. Sci. 16 (2015) 13561-13578.

Show More Hide
Cited By:
This article has no citations.