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The Characteristics of Growth of Bacilli Formed Fouling on Wooden Constructions

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Abstract:

Problem of biofilm formation have a great significance for environmental microbiological research. Biospheric microorganisms can form biofilm, that provide bacteria resistance to influence of different environmental factors. Some of the most common bacteria in biosphere are bacilli, among them there are film-forming strains. Bacillus spp. ia a well-known film forming microorganisms that colonize environmental objects. The biofilm fouling of underwater elements of small wooden constructions located on the Dnieper River near the city of the Dnipro (Ukraine) was studied. It was found that biofilms from surfaces of water constructions include bacilli. It is established that the mean values of CFU in samples from running and still water were (1.81±0.52)×108 and (1.83±0.53)×108 CFU / ml respectively per area of wooden sample approximately 1 cm2, while during the laboratory cultivation of the film, formed by these cultures on the plate, the number of cells was (4.90±0.93)×107 and (4.60±1.07)×107 CFU / ml per 1 cm2 of the well’s bottom, which was an approximate limit of the content of cells of the Bacillus spp. film per unit of area.

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Periodical:
International Letters of Natural Sciences (Volume 70)
Pages:
34-39
Citation:
V. Y. Basarab et al., "The Characteristics of Growth of Bacilli Formed Fouling on Wooden Constructions", International Letters of Natural Sciences, Vol. 70, pp. 34-39, 2018
Online since:
August 2018
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[1] A.A. Protasov, Aquatic techno-ecosystems and their place in biosphere, Journal of Siberian Federal University. Biology. 4(6) (2013) 405–423. (In Russian).

[2] A.L. McLoon et al., Tracing the domestication of a biofilm forming bacterium, J. Bacteriol. 193 (2011) 2027–(2034).

[3] A.S. Labinskaya, Ye.G. Volina (Eds.), Manual on medical microbiology. General and sanitary microbiology. Book 1, Binom, Moscow, Russia, 2008. (in Russian).

[4] A.Y. Zvyagintsev, O.P. Poltarukha, S.I. Maslennikov, Fouling on technical water supply marine systems and protection method analysis of fouling on water conduits (Analytical review), Voda: Khimiya i Ekologiya. 1 (2015) 30–51. (in Russian).

[5] D. Romero et al., Amyloid fibers provide structural integrity to Bacillus subtilis biofilms, Proc. Natl. Acad. Sci. USA. 107 (2010) 2230–2234.

[6] E.A. Shank, R. Kolter, Extracellular signaling and multicellularity in Bacillus subtilis, Curr. Opin. Microbiol. 14 (2011) 741–747.

[7] E.Y. Trizna et al., Thio derivatives of 2(5H)-furanone as inhibitors against Bacillus subtilis biofilms, Acta Naturae. 7(2) (2015) 110–116. (in Russian).

[8] F. Yan et al., The comER gene plays an important role in biofilm formation and sporulation in both Bacillus subtilis and Bacillus cereus, Front. Microbiol. 7 (2016) Article 1025.

DOI: https://doi.org/10.3389/fmicb.2016.01025

[9] H. Vlamakis et al., Sticking together: Building a biofilm the Bacillus subtilis way, Nat. Rev. Microbiol. 11(3) (2013) 157–168.

DOI: https://doi.org/10.1038/nrmicro2960

[10] I.V. Burkovskiy, Marine biogeocenology. Organization of communities and ecosystems, Partnership of Scientific Publications KMK, Moscow, Russia, 2006. (in Russian).

[11] J. Dervaux, J.C. Magniez, A. Libchaber, On growth and form of Bacillus subtilis biofilms, Interface Focus. 4 (2014) 20130051.

DOI: https://doi.org/10.1098/rsfs.2013.0051

[12] J.E. Cassat, M.S. Smeltzer, C.Y. Lee, Investigation of biofilm formation in clinical isolates of Staphylococcus aureus, in: J. Yinduo (Ed.), Methicillin-resistant Staphylococcus aureus (MRSA). Protocols, Methods in Molecular Biology, Humana press, St. Paul, (2014).

DOI: https://doi.org/10.1385/1-59745-468-0:127

[13] J.G. Holt et al. (Eds.), Bergey's manual of determinative bacteriology, Williams & Wilkins, Baltimore, (1994).

[14] K. Kobayashi, Bacillus subtilis pellicle formation proceeds through genetically defined morphological changes, J. Bacteriol. 189 (13) (2007) 4920–4931.

DOI: https://doi.org/10.1128/jb.00157-07

[15] L.S. Cairns, L. Hobley, N.R. Stanley-Wall, Biofilm formation by Bacillus subtilis: New insights into regulatory strategies and assembly mechanisms, Mol. Microbiol. 93 (2014) 587–598.

DOI: https://doi.org/10.1111/mmi.12697

[16] L.V. Didenko et al., Morphological features of biofilms in potentially dangerous water systems, Epidemiologiya i infektsionnyye bolezni. 1 (2012) 15–20. (in Russian).

[17] M. Asallya et al., Localized cell death focuses mechanical forces during 3D patterning in a biofilm, PNAS. 109 (46) (2012) 18891–18896.

DOI: https://doi.org/10.1073/pnas.1212429109

[18] N.A. Logan, R.C.W. Berkeley, Identification of Bacillus strains using the API system, J. Gen. Microbiol. 130 (1984) 1871–1882.

DOI: https://doi.org/10.1099/00221287-130-7-1871

[19] P.B. Beauregard et al., Bacillus subtilis biofilm induction by plant polysaccharides, Proc. Natl. Acad. Sci. USA. 110 (2013) E1621–E1630.

[20] S. Arnaouteli, C.E. MacPhee, N.R. Stanley-Wall, Just in case it rains: Building a hydrophobic biofilm the Bacillus subtilis way, Curr. Opin. Microbiol. 34 (2016) 7–12.

DOI: https://doi.org/10.1016/j.mib.2016.07.012

[21] S. Aryal, Biochemical test and identification of Bacillus subtilis, 2016. Available: www.microbiologyinfo.com/biochemical-test-and- identification-of-bacillus-subtilis.

[22] S.S. Branda et al., Fruiting body formation by Bacillus subtilis, Proc. Natl. Acad. Sci. USA. 98 (2001) 11621–11626.

[23] T. Gao et al., Alternative modes of biofilm formation by plant-associated Bacillus cereus, Microbiology Open. 4 (2015) 452–464.

[24] W. Ma et al., Bacillus subtilis biofilm development in the presence of soil clay minerals and iron oxides, npj Biofilms and Microbiomes. 3 (2017) 4.

DOI: https://doi.org/10.1038/s41522-017-0013-6

[25] Y. Chai et al., Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis, mBiо 3(4) (2012) e00184-12.

[26] Y. Chen et al., Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation, Environ. Microbiol. 15 (2013) 848–864.

DOI: https://doi.org/10.1111/j.1462-2920.2012.02860.x

[27] Z. Hong et al., Initial adhesion of Bacillus subtilis on soil minerals as related to their surface properties, Eur. J. Soil Sci. 63 (2012) 457–466.

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