Antimicrobial Activity of some Lactobacillus Species against Intestinal Pathogenic Bacteria

Tebyanian, Hamid; Bakhtiari, Afsaneh; Karami, Ali; Kariminik, Ashraf

doi:10.18052/www.scipress.com/ILNS.65.10

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Antimicrobial Activity of some Lactobacillus Species against Intestinal Pathogenic Bacteria

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Abstract:

Probiotics have antibacterial effects against pathogenic bacteria in the gut while maintaining the balance of intestinal flora such as Lactobacillus. This study aimed to evaluate the antimicrobial activity of four Lactobacillus species against intestinal pathogenic. Four different species of Lactobacillus (Lactobacillus bulgaricus (PTCC 1332), Lactobacillus casei (PTCC 1608), Lactobacillus plantarum (PTCC 1058) and Lactobacillus Fermentum (PTCC 1638)) were experimented to investigate the inhibitory activity against 4 bacterial enteric pathogens (Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Salmonella paratyphi A) which were separately inoculated in MRS medium (de Man, Rogosa and Sharpe medium) for 48 hours at 37 °C and pH 7. Our results showed that enteropathogens growth was stopped in the presence of all Lactobacillus and inhibition zone was between 12 and 32 millimeter. It can be concluded that these four Lactobacillus strains had potential antimicrobial compounds against human enteric pathogens and should be further studied for their human health benefits.

Info:

Periodical:

International Letters of Natural Sciences (Volume 65)

Pages:

10-15

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.65.10

Citation:

H. Tebyanian et al., "Antimicrobial Activity of some Lactobacillus Species against Intestinal Pathogenic Bacteria", International Letters of Natural Sciences, Vol. 65, pp. 10-15, 2017

Online since:

September 2017

Authors:

Hamid Tebyanian, Afsaneh Bakhtiari, Ali Karami, Ashraf Kariminik

Keywords:

Antibacterial Activity, Enteropathogenic Bacteria, Probiotics

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Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] V. Lievin-Le Moal, A.L. Servin, The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota, Clin. Microbiol. Rev. 19(2) (2006) 315-37.

DOI: https://doi.org/10.1128/cmr.19.2.315-337.2006

[2] H.L. Turner, J.R. Turner, Good fences make good neighbors: Gastrointestinal mucosal structure, Gut Microbes. 1(1) (2010) 22-29.

DOI: https://doi.org/10.4161/gmic.1.1.11427

[3] I. Yang, S. Nell, S. Suerbaum, Survival in hostile territory: the microbiota of the stomach, FEMS Microbiology Reviews. 37(5) (2013) 736-761.

DOI: https://doi.org/10.1111/1574-6976.12027

[4] H. Yazdi et al., The effects of some physicochemical stresses on Escherichia coli O157: H7 as clinical pathogenic bacteria, International Journal of Agriculture & Biology. 18(6) (2016).

[5] H. Tebyanian et al., Isolation and identification of Mycoplasma synoviae from suspected ostriches by polymerase chain reaction, in Kerman Province, Iran, Jundishapur Journal of Microbiology. 7(9) (2014).

DOI: https://doi.org/10.5812/jjm.19262

[6] P. Zarparvar et al., Isolation and identification of culturable halophilic bacteria with producing hydrolytic enzyme from incheh Broun hypersaline wetland in Iran, Cellular and Molecular Biology (Noisy-le-Grand, France). 62(12) (2016) 31-36.

[7] A. Karami et al., Planarians: an in vivo model for regenerative medicine, International Journal of Stem Cells. 8(2) (2015) 128.

[8] F. Guarner, J. -R. Malagelada, Gut flora in health and disease, The Lancet. 361(9356) (2003) 512-519.

DOI: https://doi.org/10.1016/s0140-6736(03)12489-0

[9] R.D. Rolfe, The role of probiotic cultures in the control of gastrointestinal health, The Journal of Nutrition. 130(2) (2000) 396-396.

[10] Å. Sullivan, C.E. Nord, Probiotics and gastrointestinal diseases, Journal of Internal Medicine. 257(1) (2005) 78-92.

[11] A.A. Amara, A. Shibl, Role of probiotics in health improvement, infection control and disease treatment and management, Saudi Pharmaceutical Journal. 23(2) (2015) 107-114.

DOI: https://doi.org/10.1016/j.jsps.2013.07.001

[12] G. Reid, J. Burton, Use of Lactobacillus to prevent infection by pathogenic bacteria, Microbes and Infection. 4(3) (2002) 319-324.

DOI: https://doi.org/10.1016/s1286-4579(02)01544-7

[13] D.W.K. Acheson, S. Luccioli, Mucosal immune responses, Best Practice & Research Clinical Gastroenterology. 18(2) (2004) 387-404.

DOI: https://doi.org/10.1016/j.bpg.2003.11.002

[14] A.A. Amara, The inevitability of balanced lives: genes-foods - action-interactions, IIOB Journal. 4(2) (2013) 1-27.

[15] G. Zanello et al., Saccharomyces boulardii effects on gastrointestinal diseases, Curr. Issues Mol. Biol. 11(1) (2009) 47-58.

[16] R.J. Boyle, R.M. Robins-Browne, M.L.K. Tang, Probiotic use in clinical practice: what are the risks?, The American Journal of Clinical Nutrition. 83(6) (2006) 1256-1264.

[17] S.S. Narayan et al., Probiotics: current trends in the treatment of diarrhoea, Hong Kong medical journal. 16(3) (2010) 213-218.

[18] V.C. Suvarna, V.U. Boby, Probiotics in human health: A current assessment, Current Science. 88(11) (2005) 1744-1748.

[19] V. Delcenserie et al., Immunomodulatory effects of probiotics in the intestinal tract, Current Issues in Molecular Biology. 10(1/2) (2008) 37.

[20] S. Moslehi-Jenabian, L.L. Pedersen, L. Jespersen, Beneficial effects of probiotic and food borne yeasts on human health, Nutrients. 2(4) (2010) 449-473.

DOI: https://doi.org/10.3390/nu2040449

[21] H. Awad et al., Comparison between killed and living probiotic usage versus placebo for the prevention of necrotizing enterocolitis and sepsis in neonates, Pak. J. Biol. Sci. 13(6) (2010) 253-262.

DOI: https://doi.org/10.3923/pjbs.2010.253.262

[22] S. Ammor et al., Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: Screening and characterization of the antibacterial compounds, Food Control. 17(6) (2006).

DOI: https://doi.org/10.1016/j.foodcont.2005.02.007

[23] J. -H. Park et al., Antimicrobial effect of lactic acid producing bacteria culture condensate mixture (LCCM) against Salmonella enteritidis, International Journal of Food Microbiology. 101(1) (2005) 111-117.

DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.11.005

[24] C.N. Jacobsen et al., Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans, Applied and Environmental Microbiology. 65(11) (1999).

[25] G. Reid et al., Potential uses of probiotics in clinical practice, Clin. Microbiol. Rev. 16(4) (2003) 658-672.

[26] O.V. Rybal'chenko, V.M. Bomdarenko, N.B. Verbitskaia, Antagonistic effect of bacteriocinogenic Lactobacillus acidophilus on Klebsiella pneumoniae, Citrobacter freundii and Proteus mirabilis cells, Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii. (7) (2006).

[27] A. Savadogo et al., Antimicrobial activities of lactic acid bacteria strains isolated from Burkina Faso fermented milk, Pakistan Journal of Nutrition. 3(3) (2004) 174-179.

DOI: https://doi.org/10.3923/pjn.2004.174.179

[28] H. Jamalifar et al., Antimicrobial activity of different Lactobacillus species against multi- drug resistant clinical isolates of Pseudomonas aeruginosa, Iranian Journal of Microbiology. 3(1) (2011) 21-25.

[29] L. Drago et al., Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin, FEMS Microbiology Letters. 153(2) (1997) 455-463.

DOI: https://doi.org/10.1016/s0378-1097(97)00289-9

[30] A. Santos et al., The antimicrobial properties of different strains of Lactobacillus spp. isolated from kefir, Systematic and Applied Microbiology. 26(3) (2003) 434-437.

DOI: https://doi.org/10.1078/072320203322497464

[31] S. Dasari et al., Antimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections, Asian Pacific Journal of Tropical Disease. 4(1) (2014) 18-24.

DOI: https://doi.org/10.1016/s2222-1808(14)60307-8

[32] S. Santosa, E. Farnworth, P.J.H. Jones, Probiotics and their potential health claims, Nutrition Reviews. 64(6) (2006) 265-274.

DOI: https://doi.org/10.1111/j.1753-4887.2006.tb00209.x

[33] K. Harish, T. Varghese, Probiotics in humans–evidence based review, Calicut Med. J. 4(4) (2006) e3.

[34] H. Szajewska, J.Z. Mrukowicz, Probiotics in the treatment and prevention of acute infectious diarrhea in infants and children: a systematic review of published randomized, double-blind, placebo-controlled trials, Journal of Pediatric Gastroenterology and Nutrition. 33(2) (2001).

DOI: https://doi.org/10.1097/00005176-200110002-00004

[35] A. Davoodabadi et al., Antimicrobial activity of Lactobacillus spp. isolated from fecal flora of healthy breast-fed infants against diarrheagenic Escherichia coli, Jundishapur J. Microbiol. 8(12) (2015) e27852.

DOI: https://doi.org/10.5812/jjm.27852

[36] H. Urbancsek et al., Results of a double-blind, randomized study to evaluate the efficacy and safety of Antibiophilus® in patients with radiation-induced diarrhoea, European Journal of Gastroenterology & Hepatology. 13(4) (2001) 391-396.

DOI: https://doi.org/10.1097/00042737-200104000-00015

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