Mutagenic Effectiveness and Efficiency of Gamma Rays and HZ with Phenotyping of Induced Mutations in Lentil Cultivars

Laskar, Rafiul Amin; Khan, Samiullah

doi:10.18052/www.scipress.com/ILNS.64.17

ILNS > ILNS Volume 64 > Mutagenic Effectiveness and Efficiency of Gamma...

< Back to Volume

Mutagenic Effectiveness and Efficiency of Gamma Rays and HZ with Phenotyping of Induced Mutations in Lentil Cultivars

Full Text PDF

Abstract:

In mutation breeding, mutagenic effectiveness and efficiency are the base parameters to predict the mutagenic potency of any mutagen. Studies on mutagenic effectiveness and mutagenic efficiency of physical mutagen (gamma rays) and chemical mutagen (hydrazine hydrates; HZ) on two cultivars of lentil (Lens culinaris Medik.), viz. DPL 62 (macrosperma) and Pant L 406 (microsperma) have been reported. Dry and healthy seeds were treated with four doses of each gamma rays (100-400 Gy), HZ (0.1-0.4 %) and their combinations. Frequencies of the induced agro-morphological variations into different phenotypic categories were estimated in M₂ population that resulted into identification and isolation of wide range of mutants with altered phenotypes. Data on effectiveness and efficiency of various mutagenic treatments calculated on the basis of the frequency of chlorophyll mutations, which showed effectiveness and efficiency were higher at the moderate doses of gamma rays and HZ, while in case of combination treatments; lower doses were most effective and efficient with few inter-varietal exceptions. Phenotyping of the mutants revealed that growth habits was the most sensitive category to which most of the mutant belongs, followed by leaf and flower/pod/seed in both the cultivars studied. Overall, the screened and isolated mutants with economically important agronomic traits can be further propagated in the subsequent generation for development of elite lentil mutant cultivars.

Info:

Periodical:

International Letters of Natural Sciences (Volume 64)

Pages:

17-31

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.64.17

Citation:

R. A. Laskar and S. Khan, "Mutagenic Effectiveness and Efficiency of Gamma Rays and HZ with Phenotyping of Induced Mutations in Lentil Cultivars", International Letters of Natural Sciences, Vol. 64, pp. 17-31, 2017

Online since:

July 2017

Authors:

Rafiul Amin Laskar, Samiullah Khan

Keywords:

Biological Damage, Chlorophyll Mutations, Lentil (Lens culinaris Medik.), Mutant Phenotyping, Phenotype Category

Export:

RIS, BibTeX, Text

Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] K. Arumuganathan, E.D. Earle, Nuclear DNA content of some important plant species, Plant Mol Biol. 9(3) (1991) 208-218.

DOI: https://doi.org/10.1007/bf02672069

[2] O.H. Barulina, Lentils of the USSR and other countries, Bulletin of Applied Botany, Genetics and Plant Breeding. 40 (1930) 1–319.

[3] J.I. Cubero, Origin, domestication and evolution, in: C. Webb, G.C. Hawtin (Eds), Lentils. Commonwealth Agricultural Bureau, Slough, UK, 1981, pp.15-38.

[4] W. Erskine, S. Rihawe, B.S. Capper, Variation in lentil straw quality, Animal Feed Science and Technology. 28(1-2) (1990) 61–69.

DOI: https://doi.org/10.1016/0377-8401(90)90068-j

[5] Joint FAO/IAEA Mutant Variety Database, (2017).

[6] R.A. Laskar et al., Induction of mutagenized tomato populations for investigation on agronomic traits and mutant phenotyping, Journal of the Saudi Society of Agricultural Sciences. (2016) In Press. Doi: 10. 1016/j. jssas. 2016. 01. 002.

DOI: https://doi.org/10.1016/j.jssas.2016.01.002

[7] R.F. Kimball, The mutagenicity of hydrazine and some of its derivatives, Mutat. Res. 39(2) (1977) 111–126.

[8] H.H. Smith, Comparative genetic effects of different physical mutagens in higher plants, in: Induced Mutations and Plant Breeding Improvement, IAEA, Vienna, 1972, pp.75-93.

[9] R. Kumar, S.C. Mani, Chemical mutagenesis in Manhar variety of rice (Oryza sativa L. ), Indian J. Genet. 57(2) (1997) 120-126.

[10] N.B. Gaikwad, V.S. Kothekar, Mutagenic effectiveness and efficiency of ethylmethane sulphonate and sodium azide in lentil (Lens culinaris Medik. ), Indian J. Genet. 64(1) (2004) 73-74.

[11] A. Kodym, R. Afza, Physical and chemical mutagenesis, Meth. Mol. Biol. 236 (2003) 189-203.

[12] C.F. Konzak et al., Efficient chemical mutagenesis, Rad. Bot. 5(Suppl. ) (1965) 49-70.

[13] M.L.H. Kaul, A.K. Bhan, Mutagenic effectiveness and efficiency of EMS, dES and gamma rays in rice, Theor. Appl. Genet. 50(5) (1977) 241-246.

DOI: https://doi.org/10.1007/bf00273758

[14] I.S. Solanki, B. Sharma, Mutagenic effectiveness and efficiency of gamma rays, ethyl imine and N-nitroso-N-ethyl urea in macrosperma lentil (Lens culinaris Medik), Indian J. Genet. 54(1) (1994) 72-76.

[15] A.K. Singh, Mutagenic effectiveness and efficiency of gamma rays and ethylmethane sulphonate in mungbean, Madras Agric. J. 94(1-6) (2007) 7-13.

[16] T.M. Shah et al., Induced genetic variability in chickpea (Cicer arietinum L. ) II. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens, Pak. J. Bot. 40(2) (2008) 605-613.

[17] C. Thilagavathi, L. Mullainathan, Isolation of macro mutants and mutagenic effectiveness, efficiency in blackgram (Vigna mungo (L. ) Hepper), Global J. Mol. Sci. 4(2) (2009) 76-79.

[18] M. Girija, D. Dhanavel, Mutagenic effectiveness and efficiency of gamma rays, ethylmethane sulphonate and their combined treatments in cowpea (Vigna unguiculata L. Walp), Global J. Mol. Sci. 4(2) (2009) 68-75.

[19] M.H. Khan, S.D. Tyagi, Studies on effectiveness and efficiency of gamma rays, EMS and their combination in soybean (Glycine max (L. ) Merrill), J. Plant Breed. Crop Sci. 2(3) (2010) 55-58.

[20] M.N. Khan, Mutagenic effectiveness and efficiency of EMS, gamma rays and their combination in black gram (Vigna mungo (L. ) Hepper), Ad. Plant Sci. 12(I) (1999) 203-205.

[21] A.S. Gautam, K.C. Sood, A.K. Richaria, Mutagenic effectiveness and efficiency of gamma rays, ethylmethane sulphonate and their synergistic effects in black gram (Vigna mungo L. ), Cytologia. 57 (1992) 85-89.

[22] S. Ganapathy et al., Isolation of macromutations and mutagenic effectiveness and efficiency in little millet varieties, World J. Agric. Sci. 4(4) (2008) 483-486.

[23] Y.S. Nerker, Mutagenic effectiveness and efficiency of gamma rays, ethylmethane sulphonate and nitroso methyl urea in Lathyrus sativus, Indian J. Genet. 37(1) (1977) 137-141.

[24] A. Sharma et al., Induced mutagenesis for improvement of garden pea, Int. J. Veg. Sci. 16 (2010) 60-72.

[25] M.H. Khan, S.D. Tyagi, Induced morphological mutants in soybean [Glycine max (L. ) Merrill], Front. Agric. China 4(2) (2010) 175-180.

DOI: https://doi.org/10.1007/s11703-009-0086-y

[26] M.R. Wani, S. Khan, M.I. Kozgar, Induced chlorophyll mutations. I. Mutagenic effectiveness and efficiency of EMS, HZ and SA in mungbean, Front. Agr. China. 5(4) (2011) 514-518.

DOI: https://doi.org/10.1007/s11703-011-1126-y

[27] S. Khan, K. Parveen, S. Goyal, Induced mutations in chickpea-morphological mutants, Front. Agric. China. 5(1) (2011) 35-39.

DOI: https://doi.org/10.1007/s11703-011-1050-1

[28] P.M. Gaur, V.K. Gour, Broad-few-leaflets and outwardly curved wings: two new mutants of chickpea, Plant Breed. 122(2) (2003) 192-194.

[29] C. Toker, A note on the evolution of kabuli chickpeas as shown by induced mutations in Cicer reticulatum Ladizinsky, Genet. Resour. Crop Evol. 56(1) (2009) 7-12.

[30] W. Gottschalk, The genetic basis of variation, in: Improving Vegetatively Propagated Crops, Academic Press Limited, London, UK, 1987, pp.317-334.

[31] B.S. Tyagi, P.K. Gupta, Induced macromutations in lentil [Lens culinaris], Lentil Experimental News Service. 18(1) (1991) 3-7.

[32] M.K. Jana, X-ray induced tall mutants of blackgram (Phaseolus mungo L. ), Curr. Sci. 32(10) (1963) 469-470.

[33] S.K. Sharma, B. Sharma, Leaf mutations induced with NMU and gamma rays in lentil (Lens culinaris Medik), Curr. Sci. 48 (1979) 916-917.

[34] V.R.K. Reddy, P.K. Gupta, Induced mutations in hexaploid triticale. Frequency and spectrum of morphological mutants, Genet. Agr. 42 (1988) 241-254.

[35] A. Satyanarayana et al., Multifoliate leaf mutants of mungbean and urdbean, Mut. Breed. Newslet. 33 (1989) 17.

[36] V.P. Singh, M. Singh, J.P. Pal, Mutagenic effects of gamma rays and EMS on frequency and spectrum of chlorophyll and macromutations in urdbean (Vigna mungo L. Hepper), Indian J. Genet. 59(2) (1999) 203-210.

DOI: https://doi.org/10.17485/ijst/2015/v8i10/54201

[37] D. Talukdar, Dwarf mutations in grass pea (Lathyrus sativus L. ): origin, morphology, inheritance and linkage studies, J. Genet. 88(2) (2009) 165-175.

DOI: https://doi.org/10.1007/s12041-009-0024-z

[38] C.F. Konzak, S.C. Woo, J. Dickey, An induced semidwarf plant height mutation in spring wheat, Wheat Inf. Serv. 28 (1969) 10-12.

[39] A. Shakoor et al., Selection for useful semi dwarf mutants through induced mutation in bread wheat, in: Proc. 5th Int. Wheat Genet. Symp., New Delhi, Vol. I, 1978, pp.23-28.

[40] R. Qin et al., Genetic analysis of a novel dominant rice dwarf mutant 986083D, Euphytica 160(3) (2008) 379-387.

[41] X.H. Zhang et al., A dwarf wheat mutant is associated with increased drought resistance and altered responses to gravity, African J. Biotech. 4(10) (2005) 1054-1057.

[42] D. Arulbalachandran, L. Mullainathan, Chlorophyll and morphological mutants of blackgram (Vigna mungo (L. ) Hepper) derived by gamma rays and EMS, J. Phytology. 1(4) (2009) 236-241.

[43] S. Goyal, S. Khan, Differential response of single and combined treatment in moist seeds of urdbean, Indian J. Bot. Res. 6(1-2) (2010) 183-188.

[44] A. Tripathi, D.K. Dubey, Frequency and spectrum of mutations induced by separate and simultaneous applications of gamma rays, ethyl methane sulphonate (EMS) in two microsperma varieties of lentil, Lentil Experimental News Service. 19(1) (1992).

[45] I.S. Solanki, B. Sharma, Induction and isolation of morphological mutations in different damage groups in lentil (Lens culinaris Medik), Indian J. Genet. 59(4) (1999) 479-485.

[46] S. Khan et al., Induction of morphological mutants in chickpea, Int. Chickpea and Pigeonpea Newslet. 11 (2004) 6-7.

[47] R.A. Nilan, Nature of induced mutations in higher plants. Induced mutations and their utilization, in: Proceed. of the Symp. Erawim Baur Gedachtnis Orle Singen IV. 1966, Acverlag Berlin, 1967, pp.5-20.

[48] I.S. Solanki, D.S. Phogat, R.S. Waldia, Frequency and spectrum of morphological mutations and effectiveness and efficiency of chemical mutagens in Macrosperma lentil, National J. Plant Improvement. 6(1) (2004) 22-25.

[49] V. Kumar et al., Characterization of prebreeding genetic stocks of urdbean (Vigna mungo L. Hepper) induced through mutagenesis, in: Q. . Shu (ed. ), Induced Plant Mutations in the Genomics Era, Food and Agriculture Organization of the United Nation, Rome, 2009, pp.391-394.

[50] G.S. Sethi, Long-penduncled mutant: a new mutant type induced in barley, Euphytica. 23 (1974) 237-239.

DOI: https://doi.org/10.1007/bf00035863

[51] D. Talukdar, A.K. Biswas, An induced internode mutant in grass pea, in: G.K. Manna, S.C. Roy, (ed. ) (Eds. ), Perspectives in Cytology and Genetics. Vol. 12, AICCG Publ., Kalyani, India, 2006, pp.267-272.

[52] J. Sjodin, Induced morphological variations in Vicia faba L., Hereditas. 67(2) (1971) 155-180.

[53] V. Marghitu, Mutagenic effect of X-rays and EMS in french bean in M3 and M4 generations, Striinte Agricole. 3 (1972) 105-109.

[54] R.K. Singh, Gamma ray induced bold seeded mutant in Vigna mungo (L. ) Hepper, Indian J. Genet. 56(1) (1996) 104-108.

[55] H.L. Thakur, G.S. Sethi, Characterization and segregation pattern of some macromutations induced in blackgram (Vigna mungo (L. ) Hepper), Indian J. Genet. 53(2) (1993) 168-173.

[56] W. Gottschalk, G. Wolff, Induced mutations in plant breeding, SpringerVerlag, Berlin, Heidelberg, New York, (1983).

[57] J.E. Gunkel, ,A.H. Sparrow, Ionizing radiations: biochemical, physiological and morphological aspects of their effect on plants, in: W. Ruhland (ed. ), Encyclopedia of Plant Physiology, Springer, Berlin, 1961, pp.555-611.

[58] S. Blixt, Mutation genetics in Pisum, Agric. Hort. Genet. 30 (1972) 1-293.

[59] C. Toker, M.I. Cagirgan, Spectrum and frequency of induced mutations in chickpea, Int. Chickpea & Pigeonpea Newslet. 11 (2004) 8-10.

[60] A.K. Datta, K. Sengupta, Induced viable macromutants in coriander (Coriandrum sativum L. ), Indian J. Genet. 62(3) (2002) 273-274.

[61] K.K. Sidorova, Influence of genotypic background on the expressivity of mutant genes of pea, Pulse Crops Newslet. 1(3) (1981) 23-24.

Show More Hide

Cited By:

[1] R. Amin Laskar, M. Wani, A. Raina, R. Amin, S. Khan, "Morphological characterization of gamma rays induced multipodding mutant (mp) in lentil cultivar Pant L 406", International Journal of Radiation Biology, p. 1, 2018

DOI: https://doi.org/10.1080/09553002.2018.1511927