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Expression of some Genes in Response to Cadmium Stress in Triticum aestivum

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Heavy metal toxicity has become a universal threat to all life forms, including plants. The main purpose of this study was to identify the gene expression profiling of MAPK, Thioredoxin, and MnSOD genes in wheat seedlings as affected by cadmium treatment. For this experiment, the quantitative Real-Time PCR on RNA isolated from shoots of wheat exposed to CdCl2 at a concentration of 100 mg/L was used. Results showed that in wheat seedling that exposed to cadmium stress for six days of beginning constant cadmium stress, Thioredoxin gene expression showed a large rise compared with the control sample, MnSOD gene expression increased compared with non-treated wheat seedling at the same times, but unlike the Thioredoxin and MnSOD genes, MAPK gene expression has no significant changes. Of course, it is possible that other times of beginning treatments (instead of six days) cause a change in this gene expression.


International Letters of Natural Sciences (Volume 63)
J. Karimi and S. Mohsenzadeh, "Expression of some Genes in Response to Cadmium Stress in Triticum aestivum", International Letters of Natural Sciences, Vol. 63, pp. 10-17, 2017
Online since:
May 2017

[1] M. Akashi, E. Nishida, Involvement of the MAP kinase cascade in resetting of the mammalian circadian clock, Genes and Development. 14 (200) 645-649.

[2] K. Apel, H. Hirt, Reactive oxygen species: metabolism, oxidative stress, and signal transduction, Annual Review of Plant Biology. 55 (2004) 373-399.

DOI: 10.1146/annurev.arplant.55.031903.141701

[3] E.S. Arner, A. Holmgren, Physiological functions of thioredoxin and thioredoxin reductase, European Journal of Biochemistry. 267 (2000) 6102-6109.

DOI: 10.1046/j.1432-1327.2000.01701.x

[4] J. Barcelo, C. Poschenrieder, Plant water relations as affected by heavy metal stress: a review, Journal of Plant Nutrition. 13 (1990) 1-37.

DOI: 10.1080/01904169009364057

[5] W. Ben-Qin, The research progress of the plant SOD, Journal of Hebei Agricultural Sciences. 3 (2008) 004.

[6] C. Bowler, M.V. Montagu, D. Inze, Superoxide dismutase and stress tolerance, Annual Review of Plant Biology. 43 (1992) 83-116.

DOI: 10.1146/annurev.pp.43.060192.000503

[7] Y. Chen et al., Physiological mechanism of plant roots exposed to cadmium, Chemosphere. 50 (2003) 789-793.

[8] A.A. Dalvi, S.A. Bhalerao, Response of plants towards heavy metal toxicity: an overview of avoidance, tolerance and uptake mechanism, Annals of Plant Sciences. 2 (2013) 362-368.

[9] R.T. Di Giulio et al., Biochemical responses in aquatic animals: a review of determinants of oxidative stress, Environmental Toxicology and Chemistry. 8 (1989) 1103-1123.

DOI: 10.1897/1552-8618(1989)8[1103:briaaa];2

[10] M. Doi et al., Light-inducible and clock-controlled expression of MAP kinase phosphatase 1 in mouse central pacemaker neurons, Journal of Biological Rhythms. 22 (2007) 127-139.

DOI: 10.1177/0748730406298332

[11] R. Doonan et al., Against the oxidative damage theory of aging: superoxide dismutases protect against oxidative stress but have little or no effect on life span in Caenorhabditis elegans, Genes and Development. 22(23) (2008) 3236-3241.

DOI: 10.1101/gad.504808

[12] C. Foy, R.T. Chaney, M. White, The physiology of metal toxicity in plants, Annual Review of Plant Physiology. 29 (1978) 511-566.

DOI: 10.1146/annurev.pp.29.060178.002455

[13] C.M. Grant, Role of the glutathione/glutaredoxin and thioredoxin systems in yeast growth and response to stress conditions, Molecular Microbiology. 39 (2001) 533-541.

DOI: 10.1046/j.1365-2958.2001.02283.x

[14] K.J. Livak, T.D. Schmittgen, Analysis of relative gene expression data using real-time quantitative PCR and the and the 2-ΔΔCT method, Methods. 25(4) (2001) 402-408.

DOI: 10.1006/meth.2001.1262

[15] G. Ouzounidou, M. Moustakas, E. Eleftheriou, Physiological and ultrastructural effects of cadmium on wheat (Triticum aestivum L. ) leaves, Archives of Environmental Contamination and Toxicology. 32 (1997) 154-160.

DOI: 10.1007/s002449900168

[16] A. -M.B. Pahlsson, Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants, Water, Air, and Soil Pollution. 47 (1989) 287-319.

DOI: 10.1007/bf00279329

[17] M.M. Reglero et al., Heavy metal exposure in large game from a lead mining area: effects on oxidative stress and fatty acid composition in liver, Environmental Pollution. 157 (2009) 1388-1395.

DOI: 10.1016/j.envpol.2008.11.036

[18] P. Schurmann, J. -P. Jacquot, Plant thioredoxin systems revisited, Annual Review of Plant Biology. 51(1) (2000) 371-400.

DOI: 10.1146/annurev.arplant.51.1.371

[19] A. Schutzendubel, A. Polle, Plant responses to abiotic stresses: heavy metalinduced oxidative stress and protection by mycorrhization, Journal of Experimental Botany. 53 (2002) 1351-1365.

DOI: 10.1093/jexbot/53.372.1351

[20] M.M. Siddiqui et al., Toxic effects of heavy metals (Cd, Cr and Pb) on seed germination and growth and DPPH-scavenging activity in Brassica rapa var. turnip, Toxicology and Industrial Health. 30(3) (2014) 238-249.

DOI: 10.1177/0748233712452605

[21] S. Verma, R. Dubey, Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants, Plant Science. 164 (2003) 645-655.

DOI: 10.1016/s0168-9452(03)00022-0

[22] G. Wu et al., A critical review on the bio- removal of hazardous heavy metals from contaminated soils: issues, progress, eco-environmental concerns and opportunities, Journal of Hazardous Materials. 174 (2010) 1-8.

DOI: 10.1016/j.jhazmat.2009.09.113

[23] T. Xia et al., Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties, ACS Nano. 2(10) (2008) 2121-2134.

DOI: 10.1021/nn800511k

[24] C. Xie et al., Effects of multigenerational cadmium stress on cadmium and free amino acid in hemolymph of the Musca domestica larvae, Journal of Environment and Health. 10 (2013) 009.

[25] H. -C. Yen et al., The protective role of manganese superoxide dismutase against adriamycin-induced acute cardiac toxicity in transgenic mice, Journal of Clinical Investigation. 98(5) (1996) 1253.

DOI: 10.1172/jci118909
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