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Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by Aspergillus niger

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Abstract:

Abstract- The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimization of physical and chemical parameters was done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 – 9), incubation temperature (20 – 30 °C), incubation time (8 – 80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

Info:

Periodical:
International Letters of Natural Sciences (Volume 60)
Pages:
18-29
Citation:
A. I. El-Batal et al., "Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by Aspergillus niger", International Letters of Natural Sciences, Vol. 60, pp. 18-29, 2016
Online since:
Nov 2016
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[1] T. Boonchaidung, T. Papone, R. Leesing, Effect of Carbon and Nitrogen Sources on Lipase Production by Isolated Lipase-Producing Soil Yeast, Journal of Life Sciences and Technologies. 1 (2013).

[2] H. Chahinian et al., Production of extracellular lipases by Penicillium cyclopium purification and characterization of a partial acylglycerol lipase, Bioscience, biotechnology, and biochemistry. 64 (2000) 215-222.

[3] J. Cordova et al., Lipase production by solid state fermentation of olive cake and sugar cane bagasse, Journal of Molecular Catalysis B: Enzymatic. 5 (1998) 75-78.

[4] R. Eitenmiller, J. Vakil, K. Shahani, Production and properties of Pencillium roqueforti lipase, Journal of food science. 35 (1970) 130-133.

[5] M. Elibol, D. Ozer, Influence of oxygen transfer on lipase production by Rhizopus arrhizus, Process Biochemistry. 36 (2000) 325-329.

[6] V.R. Murty, J. Bhat, P. Muniswaran, Hydrolysis of oils by using immobilized lipase enzyme: a review, Biotechnology and Bioprocess Engineering. 7 (2002) 57-66.

[7] Y. Ren et al., Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating, BMC biotechnology. 11 (2011) 1.

[8] Y. Wang, Y. -L. Hsieh, Immobilization of lipase enzyme in polyvinyl alcohol (PVA) nanofibrous membranes, Journal of Membrane Science. 309 (2008) 73-81.

[9] T. Samukawa et al., Pretreatment of immobilized Candida antarctica lipase for biodiesel fuel production from plant oil, Journal of bioscience and bioengineering. 90 (2000) 180-183.

[10] A. Macario et al., Increasing stability and productivity of lipase enzyme by encapsulation in a porous organic–inorganic system, Microporous and Mesoporous Materials. 118 (2009) 334-340.

[11] L. Wallinder et al., Hepatic and extrahepatic uptake of intravenously injected lipoprotein lipase, Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism. 795 (1984) 513-524.

[12] F. Yagiz, D. Kazan, A.N. Akin, Biodiesel production from waste oils by using lipase immobilized on hydrotalcite and zeolites, Chemical Engineering Journal. 134 (2007) 262-267.

[13] F. Ma, M.A. Hanna, Biodiesel production: a review, Bioresource technology. 70 (1999) 1-15.

[14] E. Sherwin, Oxidation and antioxidants in fat and oil processing, Journal of the American Oil Chemists' Society. 55 (1978) 809-814.

[15] F. Hasan, A.A. Shah, A. Hameed, Industrial applications of microbial lipases, Enzyme and Microbial technology. 39 (2006) 235-251.

[16] R. Sharma, Y. Chisti, U.C. Banerjee, Production, purification, characterization, and applications of lipases, Biotechnology advances. 19 (2001) 627-662.

[17] K. Jaeger, B. Dijkstra, M. Reetz, Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases, Annual Reviews in Microbiology. 53 (1999) 315-351.

[18] A. Houde, A. Kademi, D. Leblanc, Lipases and their industrial applications, Applied biochemistry and biotechnology. 118 (2004) 155-170.

[19] A. Macrae, R. Hammond, Present and future applications of lipases, Biotechnology and Genetic Engineering Reviews. 3 (1985) 193-218.

[20] E.M. Anderson, K.M. Larsson, O. Kirk, One biocatalyst–many applications: the use of Candida antarctica B-lipase in organic synthesis, Biocatalysis and Biotransformation. 16 (1998) 181-204.

[21] N.N. Gandhi, Applications of lipase, Journal of the American Oil Chemists' Society. 74 (1997) 621-634.

[22] K. -E. Jaeger, M.T. Reetz, Microbial lipases form versatile tools for biotechnology, Trends in biotechnology. 16 (1998) 396-403.

[23] P. Ghosh et al., Microbial lipases: production and applications, Science Progress (1933-), (1996) 119-157.

[24] M. Kapoor, M.N. Gupta, Lipase promiscuity and its biochemical applications, Process Biochemistry. 47 (2012) 555-569.

[25] R. Aravindan, P. Anbumathi, T. Viruthagiri, Lipase applications in food industry, Indian Journal of Biotechnology. 6 (2007) 141.

[26] E. Santaniello, P. Ferraboschi, P. Grisenti, Lipase-catalyzed transesterification in organic solvents: applications to the preparation of enantiomerically pure compounds, Enzyme and microbial technology. 15 (1993) 367-382.

[27] P.D. de María et al., Biotechnological applications of Candida antarctica lipase A: state-of-the-art, Journal of molecular catalysis b: enzymatic. 37 (2005) 36-46.

[28] M.C. Flickinger, Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology. 7 Volume Set, (2010).

[29] K. -E. Jaeger et al., Bacterial lipases for biotechnological applications, Journal of molecular catalysis B: Enzymatic. 3 (1997) 3-12.

[30] H. Fukuda, A. Kondo, H. Noda, Biodiesel fuel production by transesterification of oils, Journal of bioscience and bioengineering. 92 (2001) 405-416.

[31] A.K. Gombert et al., Lipase production by Penicillium restrictum in solid-state fermentation using babassu oil cake as substrate, Process Biochemistry. 35 (1999) 85-90.

[32] D. Pokorny, J. Friedrich, A. Cimerman, Effect of nutritional factors on lipase biosynthesis by Aspergillus niger, Biotechnology letters. 16 (1994) 363-366.

[33] K. -E. Jaeger, T. Eggert, Lipases for biotechnology, Current opinion in Biotechnology. 13 (2002) 390-397.

[34] R. Kader, A. Yousuf, M. Hoq, Optimization of lipase production by a Rhizopus MR12 in shake culture, Journal of Applied Sciences. 7 (2007) 855-860.

[35] N. Kamini, J. Mala, R. Puvanakrishnan, Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake, process Biochemistry. 33 (1998) 505-511.

[36] S. Labourdenne et al., The oil-drop tensiometer: potential applications for studying the kinetics of (phospho) lipase action, Chemistry and physics of lipids. 71 (1994) 163-173.

[37] E.W. Seitz, Industrial application of microbial lipases: a review, Journal of the American Oil Chemists' Society. 51 (1974) 12-16.

[38] M.A. Kashmiri, A. Adnan, B.W. Butt, Production, purification and partial characterization of lipase from Trichoderma viride, African Journal of Biotechnology. 5 (2006).

[39] S. Kumar et al., Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3, Protein Expression and Purification. 41 (2005) 38-44.

[40] M. Stoytcheva et al., Analytical methods for lipases activity determination: A review, Current Analytical Chemistry. 8 (2012) 400-407.

[41] V.M. Lima et al., Effect of nitrogen and carbon sources on lipase production by Penicillium aurantiogriseum, Food Technology and Biotechnology. 41 (2003) 105-110.

[42] L. Toscano et al., Production and partial characterization of extracellular lipase from Trichoderma harzianum by solid-state fermentation, Biotechnology & Biotechnological Equipment. 27 (2013) 3776-3781.

[43] A.V. Prabhu et al., Rice bran lipase: extraction, activity, and stability, Biotechnology progress. 15 (1999) 1083-1089.

[44] A.A. Khaskheli et al., Monitoring the Rhizopus oryzae lipase catalyzed hydrolysis of castor oil by ATR-FTIR spectroscopy, Journal of Molecular Catalysis B: Enzymatic. 113 (2015) 56-61.

[45] M. Valeria et al., Production of Lipase by Penicillium aurantiogriseum, Food Technol. Biotechnol. 41 (2003) 105-110.

[46] N. Mahanta, A. Gupta, S. Khare, Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate, Bioresource technology. 99 (2008) 1729-1735.

[47] R. Muralidhar et al., A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources, Biochemical Engineering Journal. 9 (2001) 17-23.

[48] A. Pandey et al., The realm of microbial lipases in biotechnology, Biotechnology and applied biochemistry. 29 (1999) 119-131.

[49] M. Prasad, K. Manjunath, Effect of media and process parameters in the enhancement of extracellular lipase production by bacterial isolates from industrial effluents, International Journal of Microbiology Research. 4 (2012) 308.

[50] S. Ushio et al., Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein, The Journal of Immunology. 156 (1996) 4274-4279.

[51] S.B. Imandi et al., Application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste, Bioresource technology. 99 (2008) 4445-4450.

[52] S.B. Imandi, S.K. Karanam, H.R. Garapati, Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus), Brazilian Journal of Microbiology. 44 (2013).

[53] A. El-Batal, H.A. Karem, Phytase production and phytic acid reduction in rapeseed meal by Aspergillus niger during solid state fermentation, Food Research International. 34 (2001) 715-720.

[54] S.K. Karanam, N.R. Medicherla, Enhanced lipase production by mutation induced Aspergillus japonicus, African Journal of Biotechnology. 7 (2008) 2064-(2067).

[55] M. Irfan, J. Javed, Q. Syed, UV mutagenesis of Aspergillus niger for enzyme production in submerged fermentation, Pak. J. Biochem. Mol. Biol. 44 (2011) 137-140.

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