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Influence of Biofertilizers on Protein, Moisture and Ash Content in Relation to Swelling Property, Water Absorption Capacity, Mineral Elements, Total Phenolic Level of Stevia (Stevia rebaudiana Bert.) Plant Grown under Acidic Soil Zone of South India

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The field experiment on Stevia rebaudiana (SR), (Family: Asteraceae) was carried out in acid soil zone of Shimoga (Karnataka) in the year 2009 and 2010 to investigate the influence of biofertilizers on protein, moisture and ash content in the dried Stevia leaf and their correlation with swelling property, water absorption capacity and mineral element contents. Results revealed that second harvested sample (August) in first year (2009) was better for all aspects than other harvested samples and the results showed significant increase in protein content (16.22%), swelling index (SI) (5.10% w/w), water absorption capacity (WAC) (4.91 ml/g) with the treatment T8 where three bio fertilizers were applied togetherly with simultaneous moisture content varied from 6.61 to 7.18%. The significant higher correlations were observed (significant at 1%) between protein content with SI (r = 0.99), WAC (r = 0.99) and SI with WAC (r = 0.98) in 2009 harvested samples. The ash content was higher in third harvested sample (13.54%) in the year 2009 with simultaneous increased amount of Fe (6.19 mg/kg), Mn (1.24 mg/kg), Cu (0.72 mg/kg) and Zn (1.22 mg/kg) contents due to the residual effect of bio fertilizers. Nitrogen (0.74 mg/kg), P (0.40 mg/kg) and K (0.68 mg/kg) contents were higher during second harvested samples and thereafter decreased. Total phenolic content was also recorded highest (64.52 mg/g) with three bio fertilizers applied togetherly during year 2009 compared to the year 2010 (50.12 mg/g). The results suggest that the application of bio-fertilizers either single or more numbers in a balanced way may be beneficial in improving protein, phenol and mineral contents of stevia plant


International Letters of Natural Sciences (Volume 6)
K. Das and R. Dang, "Influence of Biofertilizers on Protein, Moisture and Ash Content in Relation to Swelling Property, Water Absorption Capacity, Mineral Elements, Total Phenolic Level of Stevia (Stevia rebaudiana Bert.) Plant Grown under Acidic Soil Zone of South India", International Letters of Natural Sciences, Vol. 6, pp. 78-97, 2014
Online since:
November 2013

A. Abou-Arab, A. Abou-Arab, M. F. Abu-Salem, African Journal of Food Science 4 (2010) 269-281.

M. Anjanappa, J. Venkatesha, B. Suresh Kumara, Vegetable Science 38 (2011) 58-62.

AOAC, Official methods of Analysis of the Association of Official Analytical Chemists (15th edition), Washington DC. 992-995, 1990, USA.

AOAC, Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, 780, 1995, USA.

AOAC, Association of Official Analytical Chemists. 17th Edn., Official Methods of Analysis of the Association of Official Analytical Chemists International 17th Ed. Published by the Association of Official Analytical Chemists International, Suite 400, 2200 Wilson Boulevard, Arlington, Virginia 22201-3301. 2000, USA.

T. Cupic, S. Grljusic, S. Popovic, M. Stjepanovic, M. Tucak, Delgado I. (ed. ), Lloveras J. (ed. ). Quality in lucerne and medics for animal production . Zaragoza : CIHEAM. 45 (2001).

K. Das, R. Dang, T. N. Shivananda, Archives of Agronomy and Soil Science 55 (2009) 359-366.

A. Dhiman, A. Nanda, S. Ahmad, S, Toxicol Int. 18 (2011) 163-167.

P. D. Duh, Y. Y. Tu, G. C. Yen, Lebensm. -Wiss. Technol 32 (1999) 269-277.

F. E. M. El-Quesni, Kh. I. Hashish, M. M. Kandil, A. A. M. Mazher, World Applied Sciences Journal 21 (2013) 927-932.

D. Fronza, M. V. Folegatti, Scientia Agricola 60 (2003) 595-599.

X. Gao, M. Ohlander, N. Jeppsson, L. Bjork, V. Trajkovski, J. Agric. Food Chem 48 (2000) 1485-1490.

S. Ghanta, A. Banerjee, A. Poddar, S. Chattopadhyay, Journal of Agricultural Food Chemistry 55 (2007) 10962-10967.

S. Goyal, S. Samsher, R. Goyal, International Journal of Food Sciences and Nutrition 61 (2010) 1-10.

T. Hatano, R. Edamatsu, A. Mori, Chem. Pharm. Bull 37 (1989) 2016-(2021).

P. C. H. Hollman, M. G. L. Hertog, M. B. Katan, Food Chem 57 (1996) 43-46.

R. C. Huffaker, L. W. Peterson, Ann. Rev. P1. Physiol 25 (1974) 363-392.

S. Javed, A. Panwar, Recent Research in Science and Technology 5 (2013) 40-44.

F.O. Jimoh, A.T. Oladiji, Africani. Biotechnol 4 (2005) 1439-1442.

D. Kim, S. Jeong, C. H. Lee, Food Chem 81 (2003) 321-326.

N. W. Megeji, J. K. Kumar, V. Singh, V. K. Kaul, P. S. Ahuja, Current Sci. 88 (2005) 801-804.

P. Mishra, R. Singh, U. Kumar, V. Prakash, Global Journal of Biotecnology & Biochemistry 5 (2010) 62-74.

D. Mowrey, Op. Cit (1992) 1-14.

P. K. Mukherjee, Quality Control of Herbal Drugs, Business Horizons Pharmaceutical Publishers, G-59, Masjid Moth, GK-2, New Delhi - 110048, 2nd Reprint Edn, 167, 438448 (2007) 452.

G. G. E. Osuagwu, H. O. Edeoga, Int. J. Med. Arom. Plant 2 (2012) 254-262.

N. M. Patil, Recent Research in Science and Technology 2 (2010) 42-44.

I. Prakash, G. Dubois, J. Clos, K. Wilkens, L. Fosdick, Food and Chemical Toxicology 46 (2008) S75-S82.

A. Rakshit, P. B. S. Bhadoria, D. K. Das, J Interacademica 6 (2002) 570-574.

C. Rice-Evans, N. Miller, G. Paganga, Free Radical Biology and Medicine 20 (1996) 933-956.

R. D. Rosario, D. M. Flores, J. Sci. Food Agric 32 (1981) 175-180.

S. Savita, K. Sheela, S. Sunanda, A. Shankar, P. Ramakrishna, Journal of Human Ecology 15 (2004) 261-264.

E. T. Sebetha, V. I. Ayodele, F. R. Kutu, I. K. Mariga, African Journal of Biotechnology 9 (2010) 628-634.

S. Shukla, A. Mehta, V. K. Bajpai, S. Shukla, Food and Chemical Toxicology 47 (2009) 2338-2343.

K. Slinkard, V. L. Singleton, Am. J. Enol. Viticult 28 (1977) 49-55.

S. E. Smith, D. J. Read, Mycorrhizal Symbiosis, 2nd Edn. Academic Press, San Diego (1997) 126.

D. Soejarto, A. Kinghorn (Ed. ), Stevia: The genus Stevia. London, New York: Taylor and Francis (2002) 18-39.

M. Tadhani, R. Subhash, Journal of Medical Sciences 6 (2006) 321-326.

M. B. Tadhani, V. H. Patel, R. Subhash, Journal of Food Composition and Analysis 20 (2007) 323-329.

F. K. Zengin, S. Kirbag, Journal of Environmental Biology 28 (2007) 561-566. ( Received 25 November 2013; accepted 29 November 2013 ).

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