Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > Volume 57 > A Study on Extracellular Synthesis of Silver...
< Back to Volume

A Study on Extracellular Synthesis of Silver Nanoparticles from Endophytic Fungi, Isolated from Ethanomedicinal Plants Curcuma longa and Catharanthus roseus

Full Text PDF

Abstract:

Biological method is considered as eco-friendly and reliable process for the synthesis of silver nanoparticles (AgNps) in the field of nanotechnology due to its tremendous applications in various fields. In this study we have isolated a total of twelve endophytic fungi from leaves of Curcuma longa (turmeric) and Catharanthus roseus out of which six endophytic fungi showed their ability to synthesized AgNps from silver nitrate (AgNO3) solution which splits into a positive silver ion (Ag+) and a negative nitrate ion (NO3-) in order to turn the silver ions into solid silver (Ago). Of the six positive endophytic fungi VRD2 showed good and encouraging results and was identified as Penicillium spinulosum VRD2. UV-Visible Spectroscopy confirms the AgNps showing maximum peak at 425nm implying the bioreduction of AgNO3. Transmission Electron Microscopy (TEM) revealed the particle are spherical and well dispersed without agglomeration size ranging from 25-30nm.

Info:

Periodical:
International Letters of Natural Sciences (Volume 57)
Pages:
58-66
DOI:
10.18052/www.scipress.com/ILNS.57.58
Citation:
D. Singh et al., "A Study on Extracellular Synthesis of Silver Nanoparticles from Endophytic Fungi, Isolated from Ethanomedicinal Plants Curcuma longa and Catharanthus roseus", International Letters of Natural Sciences, Vol. 57, pp. 58-66, 2016
Online since:
Aug 2016
Export:
Distribution:
References:

[1] K. Kalimuthu et al, Biosynthesis of silver and gold nanoparticles using Brevibacterium casei, Colloids Surf. B: Biointerfaces. 77 (2010) 257-262. doi: 10. 1016/j. colsurfb. 2010. 02. 007.

DOI: 10.1016/j.colsurfb.2010.02.007

[2] N. Shivaraj et al, Extracellular biosynthesis of silver nanoparticles using Aspergillus flavus and their antimicrobial activity against gram negative MDR strains, Int. J. Pharm. Bio Sci. 4(2) (2013) 222–229.

[3] A. Shakeel, A. Mudasir, S. Babu Lal, I. Saiqa, A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise, Journal of Advanced Research. 7(1) (2015).

DOI: 10.1016/j.jare.2015.02.007

[4] R. Farkanda, D. Shivaji, I. Avinash, G. Aniket, R. Mahendra, Silver Nanoparticles: Novel Antimicrobial Agent Synthesized from an Endophytic Fungus Pestalotia sp. Isolated from leaves of Syzygium cumini (L), Nano Biomed. Eng. 3(3) (2011) 174-178.

DOI: 10.5101/nbe.v3i3.p174-178

[5] B. Afreen, R. Vandana, E. Ranganath., Silver nanoparticle production by Rhizopus stolonifer and antibacterial activity against extended spectrum ß- lactamase production (ESBL) strains of enterobacteriaceae, Mater Res. Bull. 46 (9) (2011).

DOI: 10.1016/j.materresbull.2011.05.008

[6] S. Sachin, K. Saranya, K. Meenal., Green synthesis of lead sulphide nanopaticles by the lead resistant marine yeast, Rhodosporidium diobovatum, Biotechnol Prog. 27(5) (2011) 1464-1469. doi: 10. 1002/btpr. 651.

DOI: 10.1002/btpr.651

[7] W. Xuetuan et al, Synthesis of silver nanoparticles by solar irradiation of cell free Bacillus amyloliquefaciens extracts and AgNO3, Bioresour Technol. 103(1) (2012) 273-278.  doi: 10. 1016/j. biortech. 2011. 09. 118.

DOI: 10.1016/j.biortech.2011.09.118

[8] S. Krishnakumar et al, Extracelluler biosynthesis of silver nanoparticles (Ag-NPs) using Fusarium oxysporium (MTCC-2480) and its antibacterial efficacy against gram negative human pathogens, J. Chem. Pharm. Res. 7 (1) (2015) 62-67.

[9] B. Valentin, G. Smriti, N.S. Usha, Silver nanoparticles synthesized from marine fungi Aspergillus oryzae, Int. J. Chem Tech Res. 7(01) (2015) 68-72.

[10] B.S. Gitanjali, M.C. Ashok, Myco-synthesis of silver nanoparticles from Trichoderma harzianum and its impact on germination status of oil seed, Biolife. 3(1) (2015) 109-113.

[11] B.K. Ravindra, A. H. Rajasab, Silver nanoparticles synthesis from different fungal species and their antifungal effect. Int J of Pharm Pharm Sci, 7(5): (2015), 165-170.

[12] S. Swetha, C.V. Nachiyar, Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus, Asian Pac. J. Trop. Biomed. 2(12) (2012) 953-959.

DOI: 10.1016/s2221-1691(13)60006-4

[13] G. Strobel et al, Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana, Microbiology. 142(2) (1996) 435–440.

DOI: 10.1099/13500872-142-2-435

[14] A. Maroof, H. Muzaffer, K. D. Monoj, K. Sanjana, Isolation of microbial endophytes from some ethnomedicinal plants of Jammu and Kashmir, J. Nat. Prod. Plant Resour. 2(2) (2012) 215-220.

[15] S. Dattu, R. Vandana, H. Jyoti, N. Shivaraj, K. Prema, Biosynthesis of silver nanoparticles by endophytic fungi Penicillium sp. Isolated from Curcuma longa (turmeric) and its antibacterial activity against pathogenic Gram negative Bacteria, Journal of Pharmacy Research. 7(2013).

DOI: 10.1016/j.jopr.2013.06.003

[16] S. Dattu, R. Vandana, N. Shivaraj, H. Jyothi, S. Ashish kumar, M. Jasmine, Optimization and characterization of silver nanoparticles by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus, Bioinorganic Chemistry and Applications, (2014).

DOI: 10.1155/2014/408021

[17] N. Nirjanta Devi, P. Dheeban Shankar, S. Sutha, Biomimetic synthesis of silver nanoparticles from an endophytic fungus and their antimicrobial efficacy, IJBAR. 03(05) (2012) 409-415. DOI: http: /dx. doi. org/10. 7439/ijbar. v3i5. 365.

DOI: 10.7439/ijbar.v3i5.365

[18] M. Joshia, A. Bhattacharayya, S. Wazed Ali, Characterization techniques for nanotechnology applications in textiles, Indian J Fibre Text. Res. 33 (2008) 304-317.

[19] R. Varshney, A. N Mishra, S. Bhadauria, M S Gaur, A novel microbial route to synthesis silver nanoparticles using fungus Hormoconis risinae, Digest Journal of Nanomaterials and Biostructures. 4(2) (2009) 349-355.

[20] U. Krebig, M. Vollmer, Optical Properties of Metal Clusters, Springer, New York, (1995).

[21] V. C Verma, R. N. Kharwar, A. C Gange, Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus, Nanomedicine. 5 (2010) 33-40.

DOI: 10.2217/nnm.09.77

[22] L. Sophiya Devi, A.B. Donal, S R Joshi, Studies on Biosynthesis of Antimicrobial Silver Nanoparticles Using Endophytic Fungi Isolated from the Ethno-medicinal Plant Gloriosa superba L, Proc Natl. Acad, Sci, India Sect, B: Biol Sci.  84(4) (2013).

DOI: 10.1007/s40011-013-0185-7

[23] V.G. Sharanabasava, B. Ravishankar, D. Raghunandan, A. Venkataraman, Extracellular biosynthesis of silver nanoparticles using fungi Penicillium diversum and their antimicrobial activity studies, BioNanoScience. 2(4) (2012) 316–321.

DOI: 10.1007/s12668-012-0046-5

[24] N. Shivaraj et al, Growth kinetics and mechanistic action of reactive oxygen species released by silver nanoparticles from Aspergillus niger on Escherichia coli, Biomed Research International. (2014).

Show More Hide