Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > ILNS Volume 54 > Recirculating Aquaculture Systems Waste Water as a...
< Back to Volume

Recirculating Aquaculture Systems Waste Water as a Medium for Increase of Phytoplankton and Zooplankton Biomass

Full Text PDF


The results of using the waste water from recirculating aquaculture system for phyto- and zooplankton cultivation are presented in the article. The physico-chemical parameters of the aquaculture system’s waste water and comparative culture media were recorded during hydrobionts cultivation. The indicators of productivity and the biochemical composition of investigated cultures were determined. The biochemical analysis of studied cultures included the investigations of the content of total proteins, lipids, carbohydrates, carotenoids and photosynthetic pigments. Using aquaculture system’s waste water as a medium for the cultivation of phyto- and zooplankton can significantly reduce the cost of technology for live feed biomass increase in the aquaculture industries.


International Letters of Natural Sciences (Volume 54)
O. Khudyi et al., "Recirculating Aquaculture Systems Waste Water as a Medium for Increase of Phytoplankton and Zooplankton Biomass", International Letters of Natural Sciences, Vol. 54, pp. 1-7, 2016
Online since:
May 2016

[1] B. Kluttgen, U. Dulmer, M. Engels, H.T. Ratte, ADaM, an artificial freshwater for the culture of zooplankton. Water Res. 28 (1994) 743-746.

[2] E.K. Zolotaryova, E.I. Shnyukova, O.O. Syvash, N. Ph. Mykhailenko, The prospects of microalgae use in biotechnology, Altpress, Kyiv, (2008).

[3] Michael B. Timmons, James M. Ebeling, Recirculating Aquaculture, NRAC Publication No 01-007, Cayuga Aqua Ventures, Ithaca, NY, (2007).

[4] O. Kushniryk, O. Khudyi, L. Khuda, R. Kolman, M. Marchenko, Cultivating Moina macrocopa Straus in different media using carotenogenic yeast Rhodotorula, Arch. Pol. Fish. 23 (2015) 37-42.

[5] L.M. Cheban, I.V. Malischuk, M.M. Marchenko, Cultivating Desmodesmus armatus (Chod. ) Hegew. in recirculating aquaculture systems (RAS) waste water, Arch. Pol. Fish. 23 (2015) 155-162.

[6] V.D. Romanenko, Yu. G. Krot, L.A. Syrenko, V.D. Solomatina, The biotechnology of hydrobionts cultivation, Institute of Hydrobiology of NAS of Ukraine, Kyiv, (1999).

[7] O.M. Arsan, O.A. Davydov, T.M. Dyachenko, M. Yu. Yevtushenko, V.M. Zhukynskyy, The methods of hydroecological investigations of surface waters, Logos, Kyiv, (2006).

[8] K. Chakri, H. Berrak, B. Samraoui, Effect of food concentration on the development, growth, reproduction and total life span of Simocephalus expinosus Koch (Cladocera: Daphniidae), Annals of Biological Research, 5 (1) (2014) 55-58.

[9] B. Khatun, R. Rahman, M.S. Rahman, Evaluation of yeast Saccharomyces cerevisiae and algae Chlorella vulgaris as diet for rotifer Brachionus calyciflorus, The Agriculturists, 12 (1) (2014) 1-9.

[10] J. Folch, M. Lees, G.H.S. Stanley, A simple method for the isolation and purification of total lipides from animal tissues, J. Biol. Chem. 226 (1957) 497-509.

[11] J.A. Knight, S. Anderson, J.M. Rawle, Chemical basis of the sulfo-phospho-vanillin. Reaction for estimating total serum lipid, Clinical Chemistry18 (1972) 199-202.

[12] O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.

[13] S.H. Roe, The determination of sugar in blood and spinal fluid with anthrone reagent, J. BioI. Chem. 212 (1955) 334-343.

[14] D.N. Campbell, V.H. Hurry, A.K. Clarke, Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation, Microbiol. Mol. Biol. Rev. 62 (1998) 667-683.

[15] M. Sanchez, C. Mantell Serrano, M. Rodriguez Rodriguez, E. Martinez de la Ossa, L. Lubian, O. Montero, Extraction of carotenoids and chlorophyll from microalgae with supercritical carbon dioxide and ethanol as cosolvent, J. Sep. Sci. 31 (2008).

[16] S.W. Geffrey, G.F. Humphrey, New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural populations, Biochem. Physiol. Pflanzen. 167 (1975) 191-194.

[17] R.P. Harris, P.H. Wiebe, J. Lenz, H.R. Skjoldal, M. Huntley, Zooplankton methodology manual, Academic Press, London, (2000).

[18] А.А. Khalafyan, STATISTICA 6. Statistical analysis of data, third ed., Binom Press, Moscow, (2007).

[19] A. Giani, Implications of phytoplankton chemical composition for zooplankton production: experimental evidence, Oecologia, 87 (1991) 409-416.

[20] M.P. Sison-Mangus, A.A. Mushegian, D. Ebert, Water fleas require microbiota for survival, growth and reproduction, The ISME Journal, 9 (2015) 59-67.

Show More Hide
Cited By:

[1] Y. Turianska, L. Cheban, M. Marchenko, "Complex evaluation of Nostoc linckia (Roth.) Born. et Flah. biomass, cultivated on waste water from recirculating aquaculture system", Biolohichni systemy, Vol. 10, p. 113, 2018


[2] L. Cheban, M. Marchenko, "Using Basaltic Tuff for Decreasing the Growth Activity of Cyanobacteria", International Letters of Natural Sciences, Vol. 78, p. 14, 2020