Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > Volume 52 > Time-Dependent Model to Mimic Acetylcholine...
< Back to Volume

Time-Dependent Model to Mimic Acetylcholine Induced Vasodilatation in Arterial Smooth Muscle Cells

Full Text PDF


Computational approaches for spatial modeling of dynamics of the intercellular distribution of molecules can parse, simplify, classify and organize the spatiotemporal richness of any biochemical pathway and demonstrate its impact on the cells function by simply coupling it with the downstream effecters. One such online system biology modeling package is Virtual cell that provides a unique open source software and it’s used for making mathematical models to simulate the cytoplasmic control of molecule that interact to produce certain cellular behavior. In our present study, a spatial model for time dependent acetylcholine induced relaxation of vascular endothelial cells lining the lumen of blood vessel that regulate the contractility of the arteries was generated. The time-dependent action of neurotransmitter acetylcholine for total time period for 1 second was studied on the endothelial cell at an interval of every 0.05 seconds. Such time simulated spatial models may be useful for testing and developing new hypotheses, interpretation of results and understand the dynamic behavior of cells.


International Letters of Natural Sciences (Volume 52)
T. R. Sahrawat and D. Chatterjee, "Time-Dependent Model to Mimic Acetylcholine Induced Vasodilatation in Arterial Smooth Muscle Cells", International Letters of Natural Sciences, Vol. 52, pp. 60-66, 2016
Online since:
Mar 2016

[1] N.A. W van Riel, Dynamic modeling and analysis of biochemical networks: mechanism-based models and model-based experiments, Briefings in bioinformatics 7(4) (2006) 364-374.

[2] L.M. Loew, J.C. Schaff, The Virtual Cell: a software environment for computational cell biology, Trends in Biotechnology 19(10) (2001) 401–406.

[3] D. Dröge, Free radicals in the physiological control of cell function, Physiological reviews, 82(1) (2002), 47-95.

[4] G.P. Robb and I. Steinberg, Visualization of the chambers of the heart pulmonary circulation and the great blood vessels in man: summary of method and results, JAMA (1940) 474-480.

[5] J.D. Coffin, T.J. Poole, Endothelial cell origin and migration in embryonic heart and cranial blood vessel development, Anat. Rec. 231(3) (1991) 383-95.

[6] D.J. Kurz, B. Naegeli, O. Bertel, A double-blind, randomized study of the effect of immediate intravenous nitroglycerin on the incidence of postprocedural chest pain and minor myocardial necrosis after elective coronary stenting, Am. Heart J. 139(1) (2000).

[7] T. Münzel, H. Li, H. Mollnau, U. Hink, E. Matheis, M. Hartmann, M. Oelze, M. Skatchkov, A. Warnholtz, L. Duncker, T. Meinertz, U. Förstermann, Effects of long-term nitroglycerin treatment on endothelial nitric oxide synthase (NOS III) gene expression, NOS III–mediated superoxide production, and vascular NO bioavailability, Circ. Res. 86 (1) (2000).

[8] R.F. Furchgott , J.V. Zawadzki, The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine, Nature 288 (1980) 373-6.

[9] R.F. Furchgott, P.D. Cherry, J.V. Zawadzki, D. Jothianandan, Endothelial cells as mediators of vasodilation of arteries, J. Cardiovasc. Pharmacol. 6(2) (1984) S336-343.

[10] Y. Hirooka, T. Imaizumi, T. Tagawa, M. Shiramoto, T. Endo, S. Ando, A. Takeshita, Effects of L-arginine on impaired acetylcholine-induced and ischemic vasodilation of the forearm in patients with heart failure, Circulation 90(2) (1984) 658-668.

[11] J.M. Berg, J.L. Tymoczko, L. Stryer, Biochemistry, fifth edition, New York: W. H. Freeman, (2002).

[12] H. Lodish, A. Berk, S.L. Zipursky, P. Matsudaira, D. Baltimore, J. Darnell, Molecular Cell Biology, fourth edition, New York: W.H. Freeman, (2000).

[13] P. Taylor, J.H. Brown, Synthesis Storage and Release of Acetylcholine, in: G.J. Siegel, B.W. Agranoff, R.W. Alberts (Eds. ), Basic Neurochemistry: Molecular, Cellular and Medical Aspects, sixth edition, Philadelphia: Lippincott-Raven, (1999).

[14] Information on http: /med. stanford. edu/news/all-news/2010/02/virtual-cell-could-bring-benefits-of-simulation-to-biology. html.

[15] M. Klem, Nitric oxide metabolism and breakdown, Biochimica et Biophysica Acta (BBA) Bioenergetics, 1411 (2)(1999) 273-289.

[16] J.T.S. Hakim, K. Sugimori, E.M. Camporesi, G. Anderson, Half-life of nitric oxide in aqueous solutions with and without haemoglobin, Physiol Meas. 17(4) (1996) 267-277.

[17] T.R. Sahrawat, S. Bhalla, Identification of Critical Target Protein for Cystic Fibrosis using Systems Biology Network Approach, Int. J. Bioautomation 17(2013) 227-240.

Show More Hide