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In Vitro Control of Tomato (Solanum lycopersicon L.) Fruit Rot Caused by Fungi Using Two Plant Extracts

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The inhibitory properties of the ethanolic and methanolic leaf extracts of Vernonia amygdalina and Cola acuminata on the fungal pathogens isolated from infected tomato fruits were investigated. The pathogens were Fusarium moniliformes and Rhizopus stolonifer. Various concentrations of the extracts ranging from 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% were separately added to PDA media. The fungal pathogens were separately inoculated into the media and incubated for seven days. Antifungal effects of these extracts on the mycelia growth of the pathogens were significant at P<0.05 for all treatments at higher concentrations. At 10-50% concentration, ethanolic and methanolic extracts of Vernonia amygdalina and Cola acuminata had no significant effect on the mycelia growth of Fusarium moniliformes and Rhizopus stolonifer after seven days observation period. At 60-100% concentrations, the two pathogens were completely inhibited by ethanolic extracts of Vernonia amygdalina and Cola acuminata. Methanolic extracts of Vernonia amygdalina and Cola acuminata inhibited completely Fusarium moniliformes and Rhizopus stolonifer at 80-100% concentrations. The in vitro inhibitory effects of these extracts at higher concentrations indicated that they can be used for the control of tomato fruit rot. It may be necessary to use them in prolonging the shelf-life of fresh tomato fruit and some other fruits.


International Letters of Natural Sciences (Volume 52)
E. N. Okey et al., " In Vitro Control of Tomato (Solanum lycopersicon L.) Fruit Rot Caused by Fungi Using Two Plant Extracts", International Letters of Natural Sciences, Vol. 52, pp. 19-27, 2016
Online since:
Mar 2016

[1] Abada, K. A., Mostafa, S.H. and Mervat, R. (2008). Effect of some chemical salts on suppressing the infection by early blight disease of tomato. Egyptian Journal of Applied Science, 23: 47-58.

[2] Ahmed, S. and Stoll, G. (1996). Biopesticides. In: Biotechnology; Building on Farmers' Knowledge. Macmillan Education Ltd, Bunders, J., B. Haverkort and W. Hiemstra (Eds. ). London, Pp: 52-79.

[3] Allen, A. (2008). A Passion for Tomatoes,. Smithsonian Magazine, 20p.

[4] Amadioha, A. C. (2000). Fungitoxic effects of some leaf extracts against Rhizopus oryzae causing tuber rot of potato. Archives of Phytopatholpflanzo, 34: 1-9.

[5] Amadioha, A. C. and Obi, V. I. (1999). Control of Anthracnose diseases of Cowpea by Cymbopogon cunitus and Ocimum gratissimum. Acto Phytopathology and Entomology, 85: 89-94.

[6] Barnett, H. L. and Hunter, B. B. (1998). Illustrated genera of imperfect fungi 4th edition, St. Paul Minnesota. APS Press. P. 32.

[7] Bowers J. H. and Locke J. C. (2004). Effect of formulated plant extracts and oils on population density of Phytophthora nicotianae in soil and control of Phytophthora blight in the greenhouse. Plant Disease, 88: 11–16.

DOI: 10.1094/pdis.2004.88.1.11

[8] Chiejina, N. V. and Ukeh, J. A. (2013). Efficacy of Afromomum melegueta and Zingiber officinale extracts on fungal pathogens of tomato fruit. IOSR Journal of Pharmacy and Biological Sciences, volume 4, issue 6: 13-16.

DOI: 10.9790/3008-0461316

[9] Chuku, E. C. and Osakwe, J. A., Daddy-west, C. (2010). Fungal spoilage of tomato (Lycopersicon esculentum mill) using Garlic and Ginger. Scientia Africana, 9(2): 41-46.

[10] Dugan, F. M., (2006). The identification of fungi. APS press, St Paul Minnesota. P. 50.

[11] Fall, P. A., Fredrikson, M., Axelson, O. and Granérus, A. K. (2009). Nutritional and occupational factors influencing the risk of Parkinson's disease: A case-control study in southeastern Sweden,. Movement Disorders, 14 (1): 28–37.

DOI: 10.1002/1531-8257(199901)14:1<28::aid-mds1007>;2-f

[12] Freedman, N. D., Park, Y, Subar, A. F., Hollenbeck, A. R., Leitzmann, M. F., Schatzkin, A, and Abnet, C. C. (2008).

[13] Gentilcore, D. (2010). A History of the Tomato in Italy Pomodoro! New York, NY: Columbia University Press, ISBN 023115206X.

[14] Ilondu, E. M., Ejechi, B. O. and Souzey, J. A., (2001). Microbial stability of jam prepared from velvet tamarind and preserved by combined processes. Nigerian Journal of Microbiology, 5: 93-96.

[15] Kagale, S., Marimuthu, T., Thayumanavan, B., Nandakumar, R. and Samiyappan, R. (2004).

[16] Kolata, G., (2012). Flavor is Price of Scarlet Hue of Tomatoes, Study Finds". The New York Times. P. 14.

[17] Mcgee, H. (2009). Accused, yes, but probably not a killer,. The New York Times. P. 5.

[18] Mehrotra, R. S. and Aggarwal, A. (2003). Plant pathology. 2nd Ed. Tata McGraw- Hill. New Delhi. P. 846.

[19] Mital, M. P., Manoranja, K. and Sahu, R. K. (2012). Bioefficacy of some plant extracts on growth parameters and control of diseases of Lycopersicon esculentum. Asian Journal of Plant Science and Research, 2 (2): 129-142.

[20] Mourvaki, E.; Gizzi, S.; Rossi, R. and Rufini, S. (2005). Passionflower Fruit — A "New" Source of Lycopene, Journal of Medicinal Food, 8(1): 104-108.

DOI: 10.1089/jmf.2005.8.104

[21] Okigbo, R. N., (2009). Variation in phytochemical properties of selected fungicidal aqueous extract of some plant leaves in Kogi state, Nigeria. American-Eurasian Journal of Sustainable Agriculture, 3 (3): 407-409.

[22] Okigbo, R. N. and Nmeka, I. A., (2005). Control of yam tuber rot with leaf extracts of Xylopia aethiopica and Zingiber officinale. African Journal of Biotechnology, 4(8), 804-807.

[23] Okigbo, R. N., Ramesh, P. and Achusi, C. T. (2009). Post-Harvest Deterioration of Cassava and its Control using extracts of Azadirachta indica and Aframomum melegueta. E- Journal of Chemistry, 6 (4), 1274-1280.

DOI: 10.1155/2009/680519

[24] Parnell, T. L., Suslow, T. V. and Harris, L. J. (2004). Tomatoes: Safe Methods to Store, Preserve, and Enjoy,. ANR Catalog. University of California: Division of Agriculture and Natural Resources. Retrieved 18 February (2013).

[25] Polivkova, Z., Smerak, P., Demova, H. and Houska, M. (2010). Antimutagenic effects of Lycopene and Tomato Puree,. Journal of Medicinal Food, 13 (6): 1443–1450.

DOI: 10.1089/jmf.2009.0277

[26] Rao, A. V. and Balachandran, B. (2002). Role of oxidative stress and antioxidants in neurodegenerative diseases,. Nutritional Neuroscience, 5 (5): 291–309.

DOI: 10.1080/1028415021000033767

[27] Santas, J., Almajano, M. P. and Carbo, R. (2010). Antimicrobial and antioxidant activity of crude onion (Allium cepa L. ) extracts. International Journal of Food Science Technology, 45: 403–409.

DOI: 10.1111/j.1365-2621.2009.02169.x

[28] Shidfar, F., Froghifar, N., Vafa, M., Rajab, A., Hosseini, S., Shidfar, S. and Gohari, M. (2011).

[29] Udo, S. E., Madunagu, B. E. and Isemin, C. D. (2001). Inhibition of growth and sporulation of fungal pathogens on sweet potato and yam by garlic extract. Nigerian Journal of Botany, 14: 35-39.

[30] Zhang, C. X., Ho, S. C., Chen, Y. M., Fu, J. H., Cheng, S. Z. and Lin, F. Y. (2009). Greater vegetable and fruit intake is associated with a lower risk of breast cancer among Chinese women,. International Journal of Cancer, 125 (1): 181–188.

DOI: 10.1002/ijc.24358
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