Respiratory Surveillance and Ca2+-ATPase Enzyme Activity Studies of Clarias gariepinus Exposed to Acute Toxicity of Cyanide

Oseni, Kadiri

doi:10.18052/www.scipress.com/ILNS.50.62

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Respiratory Surveillance and Ca²⁺-ATPase Enzyme Activity Studies of Clarias gariepinus Exposed to Acute Toxicity of Cyanide

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Abstract:

Potassium cyanide, a highly contaminating and toxic aquatic ecosystems pollutant was investigated for acute toxicity on the freshwater fish Clarias gariepinus. Its effect on the Ca²⁺ -ATPase activities in the liver, gills, muscle and intestinal tissues and oxygen consumption index was studied. Short-term toxicity test was carried out by static renewal bioassay test over a 96 h period using a lethal concentration (LC₅₀) value of 0.361mg/mL. Potassium cyanide was highly toxic to the animal tested. Results reveal that normal respiratory activity (O₂consumption) of the fish was significantly affected and there was significant decreased in the Ca²⁺ - ATPase activities at the end of exposure periods (24, 48, 72 and 96 h). Correlation analysis reveals a strong relationship between oxygen consumption index and ATPase enzyme activity of Clarias gariepinus exposed to the toxicant. This study reflects the toxic effect of potassium cyanide to the freshwater fish, Clarias gariepinus and suggestion on the possible application of Ca²⁺ -ATPase activities and oxygen consumption index as possible biomarkers for early detection of cyanide poisoning in aquatic bodies.

Info:

Periodical:

International Letters of Natural Sciences (Volume 50)

Pages:

62-69

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.50.62

Citation:

K. Oseni, "Respiratory Surveillance and Ca²⁺-ATPase Enzyme Activity Studies of Clarias gariepinus Exposed to Acute Toxicity of Cyanide", International Letters of Natural Sciences, Vol. 50, pp. 62-69, 2016

Online since:

January 2016

Authors:

Kadiri Oseni

Keywords:

Ca²⁺-ATPase, Catfish, Cyanide, LC₅₀, Toxicity

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Open Access

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Creative Commons Attribution 4.0 International License

References:

[1] Ardelt, B.K.; Borowitz, J.L.; Isom, G.E.; (1989). Brain lipid peroxidation and antioxidant defence mechanisms following acute cyanide intoxication. Toxicol., 56, 147-54.

[2] Begum, G. (2011). Organ-specific ATPase and phosphorylase enzyme activities in a food fish exposed to a carbamate insecticide and recovery response. Fish Physiology and Biochemistry, 37 (1), 61-69.

[3] Cattell, R. B. (1996). The Screen Test for the number of Factors. Multivar. Behav. Res., 1, 245.

[4] Connell, D.; Lam, P.; Richardson, B.; Wu, R. (1999). Introduction to ecotoxicology. London press, p.170.

[5] Dreisenbach, R.H.; Robertson, W.O. (1987). Handbook of poisoning: prevention, diagnosis and treatment. 12th edition. Appleton and Lange, Norwalk, CT.

[6] Dube P.N.; Hosetti, B.B. (2010). Behaviour surveillance and oxygen consumption in the freshwater fish Labeo rohita (Hamilton) exposed to sodium cyanide. Biotechnology in Animal Husbandry, 26 (1-2), 91-103. http: /dx. doi. org/2298/BAH1002091D.

[7] Finney, D.T. (1971). Probit Analysis. 3rd Ed. Cambridge University Press. London.

[8] Greer, J.J.; Jo, E. (1995). Effects of cyanide on neural mechanisms controlling breathing in neonatal rat in vivo. Neurotoxicology, 16, 211– 215.

[9] Grinwis, G.C.M.; Boonstra, A.; Vandenbrandhof, E.J.; Dormans, J.A.M.A.; Engelsma, M.; Kuiper, V.; Vanloveren, H.; Wester, P.W.; Vaal, M.A.; Vethaak, A.D.; VOS J.G. (1998).

[10] Hartl, M.G.J.; Hutchinson, S.; Hawkins, L. (2001).

[11] Heskett, J.E.; Loudon, J.B.; Reading, W.H.; Glen, A.M. (1978). The effect of lithium treatment on erythrocyte membrane ATPase activities and erythrocyte ion content. Britain Journal of Clinical Pharmacy, 5, 323–329.

[12] Holland, D.J. (1983). Cyanide poisoning: an uncommon encounter. J Emerg. Nurs., 9(3), 138.

[13] Isom, G.E.; Borowitz, J.L. (1995). Modification of cyanide toxico-dynamics: Mechanistic based antidote development. Toxicol Lett., 82/83, 795-9.

[14] Isom, G.E.; Borowitz, J.L.; Mukhopadhyay, S. (2010). Sulfurtransferase enzymes involved in cyanide metabolism. In: Charlene A. M, editor. Comprehensive Toxicology. Oxford: Elsevier. p.485–500.

[15] Jones, M.G.; Bickar, D.; Wilson, M.T.; Brunori , M.; Colosimo, A.; Sarti, P. (1984). A re-exanimation of the reactions of cyanide with cytochrome oxidase. Biochem J., 220, 56–66.

[16] Kadiri. O. (2015).

[17] Moran, J.M.; Morgan, M.D.; Wiersma, D.; James, H. (1980). Introduction to environmental science, 2nd Edn. WH Freeman, New York, NY.

[18] OECD Guidelines for Testing of Chemicals (No. 203; Adopted: 17th July, 1992).

[19] Okolie, N. P.; Audu, K. (2004). Correlation between cyanide- induced decreases in ocular Ca2+-ATPase and lenticular opacification. Journal of Biomedical Sciences, 3 (1), 37-41.

[20] Prashanth, M.S., H.A. Sayeswaraand and A.G. Mahesh 2011. Effect of Sodium Cyanide on Behaviour and Respiratory Surveillance in Freshwater Fish, Labeo Rohita (Hamilton). Recent Research in Science and Technology, 3(2), 24-30.

[21] Radhaiah, V.; Jayantha, R.K. (1988). Behavioural response of fish, Tilapia mossambica exposed to fenvalerate - Environmental Ecology, 6(2), 2-23.

[22] Ramzy, M.E. (2014). Toxicity and stability of sodium cyanide in fresh water ﬁsh Nile tilapia-Water Science 28, 42–50. http: /dx. doi. org/10. 1016/j. wsj. 2014. 09. 002.

[23] Shwetha, A.; Praveen, N.B.; Hosetti, B.B. (2012). Effect of Exposure to Sublethal Concentrations of Zinc Cyanide on Tissue ATPase Activity in the Fresh Water Fish, Cirrhinus mrigala (Ham). Acta Zoologica Bulgarica, 64 (2), 185-190.

[24] Shwetha, A.; Hosetti, B.B. (2009). Acute effects of zinc cyanide on the behaviour and oxygen consumption of the Indian major carp, Cirrhinus mrigala-World Journal of Zoology 4(3), 238-246.

[25] Solomonson, L.P. (1981). Cyanide as a metabolic inhibitor. In Cyanide in biology edited by B. Vennesland, E.E. Conn, C.J. Knowles, J. Westley and F. Wissing, San Diego, Academic Press, pp: 11-28.

[26] Tiwari, B.S.; Belenghi, B.; Levine, A. (2002).

[27] Unnisa, Z.A.; Devaraj, N.S. (2007). Effect of methacrylo-nitrile on membrane bound enzymes of rat brain. Ind. J. Physiol. Pharmacol., 51(4), 405–409.

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