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ILNS > Volume 5 > Effect of Temperature on Guaiacol Peroxidase of...
Effect of Temperature on Guaiacol Peroxidase of <i>Pyrus communis</i>
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Effect of Temperature on Guaiacol Peroxidase of Pyrus communis

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Abstract:

Peroxidase (EC 1.11.1.7; donor: hydrogen-peroxide oxidoreductase, POD) is one of the key enzymes controlling plant growth, differentiation and development. The enzyme participates in construction, rigidification and eventual lignification of cell walls, biosynthesis of ethylene from 1-aminocyclopropane-1-carboxylic acid and H2O2, regulation of auxin level through auxin catabolism, protection of tissue from damage and infection by pathogenic microorganisms, the oxidation of indoleacetic acid. For peroxidase activity in wild pears extract one pH optimum was observed at 6.5 that probably belong to atleast one isoenzyme. Activity of peroxidase in presence of guaiacol and H2O2 was optimum after incubation at 40 °C. Maximum activity of peroxidase is 300% .Activity increased to 240%, 300%, 70% and 10% after 60 minute incubation at 30, 40, 45 and 60 °C for peroxidase. Incubation at high temperature (70 °C) was accompanied with decrease of activity to 10% peroxidase activity.

Info:

Periodical:
International Letters of Natural Sciences (Volume 5)
Pages:
46-51
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.5.46
Citation:
S. Saeidian and E. Ghasemifar, "Effect of Temperature on Guaiacol Peroxidase of Pyrus communis", International Letters of Natural Sciences, Vol. 5, pp. 46-51, 2013
Online since:
November 2013
Authors:
Shahriar Saeidian, Elham Ghasemifar
Keywords:
Guaiacol, Peroxidase, Pyrus Communis, Temperature
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Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

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Cited By:

[1] d. Marcio, M. Juliana, P. Francisco, F. Natoniel, V. Katia, "Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.)", African Journal of Biotechnology, Vol. 14, p. 1838, 2015

DOI: https://doi.org/10.5897/AJB2015.14521