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Effect of Milk and Xanthan as Egg Replacement on the Physical Properties of Mayonnaise

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The milk along with xanthan at levels 0, 1.9, 3.9, 5.9, 7.9, 9.9 and 0.1 and 0.2 % (No 1 (control) to 11 respectively) as egg replacement were used for preparation of mayonnaise. Creaming index, heat stability, physical stability and rheological properties of samples over a were investigated. Also flow behavior of samples were fitted to Power law, Herschel-Bulkley and Casson models. It was found that addition milk along with xanthan to mayonnaise protected from phase separation in comparison to control sample. All the samples had stability higher than 99% except samples 1, 4, 5 and 6. The highest stability was recorded for sample No 11 (99.98%) and the lowest for 5 (74.95%) that compared with control sample had significant differences (p> 0.05). All the samples except No 1 and 5 showed heat stability higher than 99 % and the highest stability recorded for No 11 ( 99.72%) and the lowest for No 5 (67.42 %) and in comparison with control were significantly different (p> 0.05). Apparent viscosity decreased with increase the egg replacement from 40% to 80%, as the highest and lowest viscosity observed in samples contain 100% and 80% replacement respectively. All samples showed shear-thinning behavior and the high coefficients of determination for Power law and Herschel-Bulkley models (0.99) revealed the adequacy of these rheological models to describe flow behavior of mayonnaise.


International Letters of Natural Sciences (Volume 49)
M. G. Shamooshaki et al., "Effect of Milk and Xanthan as Egg Replacement on the Physical Properties of Mayonnaise", International Letters of Natural Sciences, Vol. 49, pp. 24-34, 2015
Online since:
November 2015

Abu Ghoush, M., Samhouri, M., Al-Holy, M., and Herald, T. 2008. Formulation and fuzzy modeling of emulsion stability and viscosity of a gum-protein emulsifier in a model mayonnaise system. Journal of Food Engineering, 84: 348-357.

Davis, J.P., Foegeding, E.A., Hansen, F.K., Colloids Surf. B: Biointerfaces 34 (2004) 13-23.

Dickinson, E. and Stainsby, G. (1982). Colloids in Foods. Applied Science, New York.

Ghajari shamooshaki, M., Sadeghi Mahoonak, A., Maghsoodloo, Y., and Ghorbani, M. 2015. Effect of milk and Xanthan as egg replacement on the chemical and microbial safety properties of Mayonnaise. Scholarly Journal of Agricultural Science Vol. 5(8), pp.256-261.

Goff, HD: Formation and stabilization of structure in ice cream and related products. Current Opinion Colloid Interface Sci. (2002a) In press.

Goff HD: Ice cream. In Fox PF, McSweeney PLH (eds) Advanced Dairy Chemistry - 1. Proteins, 3rd Edn. Kluwer Academic, New York (2002b).

Guerrero, A., and H.R. Ball, Effect of Spray-Dried or Reduced- Cholesterol Yolk and Temperature on the Linear Viscoelastic Properties of Mayonnaise, J. Texture Stud. 25: 363-381 (1994).

Hennoc, M., Rahalkar, R.R., and Richmond, P., 1984. Effect of xanthan gum upon the rheology and stability of oil in water emulsions. Journal of Food Science, 49, 1271-1274.

Joly, M., Surface and Colloid Science, (ed. E. Matijevic) 9, (1972). Plenum Press, New York.

Juszczak L, Fortuna T et al (2003) Sensory and rheological properties of polish commercial mayonnaise. Die Nahrung 47(4): 232-235.

Liu, H., Xu, X.M., and Guo., Sh.D., 2007. Rheological, texture and sensory properties of low fat mayonnaise with different fat mimetics. Journal of Food Science and Technology, 40: 946-954.

Mangino ME (1994) Protein interactions in emulsions: protein-lipid interactions. In: Hettiarachchy NS, Ziegler GR (eds) Protein functionality in food systems. M Dekker, New York, pp.147-180.

Marinova, K.G., Basheva, E.S., Nenova, B., Temelska, M., Mirarefi, A.Y., Campbell, B., Ivanov, I.B. Food Hydrocolloids 23 (2009) 1864.

Martinez, M.J., Carrera, C., Rodriguez Patino, J.M., Pilosof, A.M.R. Colloids Surf. B: Biointerfaces 68 (2009) 39.

Maruyama K, Sakashita T, Hagura Y and Suzuki K, 2007. Relationship between rheology, particle size and texture of mayonnaise. Food Science and Technology Research 13: 1-6.

McClements DJ., 2004. Food emulsions: principles, practices, and techniques: CRC.

McClements, DJ., 1999. Food emulsions; principles, practice, and techniques. Boca Raton, FL: CRC Press.

Mitschka, p., 1982. simple conversion of Brookfeild R.V.T. readings into viscosity functions. Rheologica Acta. 21: 207-(2090).

Mohammadzadeh, H., Koocheki, A., Kadkhodaee, R., and Razavi, S., 2013. Physical and flow properties of d-limonene-in-water emulsions stabilized with whey protein concentrate and wild sage seed gum. Food Research International, 53(1), 312-318.

Mun, S., Kim, Y.L., Kang, C., Kang, C., Shim, J., and Kim, Y., 2009. Development of reduced-fat mayonnaise using 4[alpha]GTase-modified rice starch and xanthan gum. International Journal of Biological Macromolecules. 44(5): 400-407.

Narsimhan G, Wang Z (2008) Guidelines for processing emulsion-based foods. In: Hasenhuettl GL, Hartel RW (eds) Food emulsifiers and their applications. Springer Sci BusinessMedia, New York, pp.349-389.

Nikzade, V., Mazaheri Tehrani, M., Saadatmand-Tarzjan, M., 2012. Optimization of low- cholesterol-low-fat mayonnaise formulation: Effect of using soy milk and some stabilizer by a mixture design approach. Food Hydrocolloids, 28(2): 344-352.

Paraskevopoulou, A., V. Kiosseoglou, S. Alevisopoulos, and S. Kasapis, Influence of Reduced-Cholesterol Yolk on the Viscoelastic Behaviour of Concentrated O/W Emulsions, Colloids Surf. B 12: 107-111 (1999).

Paredes, M. D. C., Rao, M. A. & Bourne, M. C. (1988). Rheological characterization of salad dressings, 1. Steady shear, thixotropy and effect of temperature. J. Texture Studies, 19, 247-58.

Perez, A, A., Sanchez, C, C., Rodriguez Patino, J, M., Rubiolo, A, C., Santiago, L,G. 2010. Milk whey proteins and xanthan gum interactions in solution and at the air-water interface: A rheokinetic study. Colloids and Surfaces B: Biointerfaces, 81: 50-57.

Perez, A.A., Carrara, C.R., Carrera, C., Santiago, L.G., Rodriguez Patino, J.M. Food Hydrocolloids 23 (2009) 1253.

Rahmati, K., Mazaheri Tehrani, M., and Daneshvar, K., 2012. Soy milk as an emulsifier in mayonnaise: physico-chemical, stability and sensory evaluation. Journal of Food Science and Technology (DOI 10. 1007/s13197-012-0806-9).

Rao, M.A., Rheological Behavior of Processed Fluid and Semisolid Foods, in Rheology of Fluid and Semisolid Foods, edited by M.A. Rao, Aspen Publishers, Gaithersburg, 1999, pp.219-254.

Raymundoa, A., Francob, J. M., Empisc, J., and Sousad, I., 2002. Optimization of the composition of cow-fat oil-in-water emulsions stabilized by white lupin protein. JAOCS, 79(8).

Rezaei, R., Khomeiri, M., Kashaninejad, M., and Aalami, M., 2011. Effects of guar gum and arabic gum on the physicochemical, sensory and flow behavior characteristicsof frozen yoghurt. International Journal of Dairy Technology, 64(4): 563-568.

Rodriguez Patino, J.M., Rodriguez Nino, M.R., Carrera, C., Curr. Opin. Colloid Interface Sci. 12 (2007) 187.

Sathivel, S., Bechtel, P., Babbitt, J., Prinyawiwatkul, W., and Patterson, M., 2005. Functional, nutritional, and rheological properties of protein powders from Arrowtooth Flounder and their application in mayonnaise. Journal of Food Engineering and Physical Properties, 70 (2): 57-63.

Srivastava, S. N., D. A. Haydon in: Chemistry, Physics and AEElications of Surface Active Substances, (ed. J. Th. G. Overbeek) p.122, (1964). Academic Press, London.

Stadelman, W. J., & Cotterill, O. J. (1995). Egg science and technology (3rd ed. ). Wesport: AVI Publischer Company.

Steffe FJ., 2006. Bioprocessing Pipelines: Rheology and Analyses. Freeman Press. USA.

Su,H., Lien,C., Lee T.A., Ho,R.S. 2010. Development of low fat mayonnaise containing polysaccharide gum as functional ingredients, JSci Food Agric.

Sun, C., Gunasekaran, S., & Richards, M. P. (2007). Effect of xanthan gum on physicochemical properties of whey protein isolate stabilized oil-in-water emulsions. Food Hydrocolloids, 21(4), 555-564.

Takeda K, Matsumura Yet al (2001) Emulsifying and surface properties of wheat gluten under acidic conditions. J Food Sci 66: 393-399.

Thompson L U, Reniers D J, 1981. Succinylated cheese whey protein concentrates in coffee whitener and salad dressing. Journal of Dairy Science 65: 1135-1140.

Walstra, P. (1986). Overview of emulsion and foam stability. In Food Emulsion and Foams, ed. E. Dickinson. The Royal Society Of Chemistry, London, p.2422257.

Yang, X., Berry, T.K., Foegeding, E.A., J. Food Sci. 74 (2009) 259.

Yilmazer, G., Carrillo, A. L. & Kokini, J. L. (1991). Effect of propylene glycol alginate and xanthan gum on stability of O/W emulsions. J. Food Sci., 56, 5 13- 17.

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