Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

Arjun,; Srinath, Rao

doi:10.18052/www.scipress.com/ILNS.48.14

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Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

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Abstract:

An efficient protocol for induction of callus and regeneration of a sugar cane var 93v297 has been developed and reported here. Callus induction from immature young leaf explants derived from 2-3-month-old plants was achieved on Murashige and Skoog’s (MS) medium supplemented with different auxins viz, 2,4-D, NAA and IAA. Among different auxins, 2, 4-D at 3.5mg/l + 0.5mg/l BAP was found favourable in inducing callus. Addition of coconut milk and BAP further enhanced the growth of callus maximum being on MS medium supplemented with 0.5mg/l BAP (3602.33±0.88mg). Calli were further evaluated for regeneration. MS medium supplemented with 1.0 mg/l BAP was found suitable where 100% calli regenerated with maximum number of multiple shoots per callus mass (41.40±0.89). Highest number of root emergence (28.33±1.16) and maximum root length (3.40±0.67cm) was achieved on MS medium supplemented with 3mgl/l NAA. The in vitro grown plants were transferred to polycups containing a mixture of sterilized sand, soil and cocopeet (1:1:1) for hardening. The hardened plants were transferred to green-house conditions where they survived with 90% frequency.

Info:

Periodical:

International Letters of Natural Sciences (Volume 48)

Pages:

14-22

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.48.14

Citation:

Arjun and R. Srinath, "Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297", International Letters of Natural Sciences, Vol. 48, pp. 14-22, 2015

Online since:

November 2015

Authors:

Arjun, Rao Srinath

Keywords:

Callus, Immature Leaf Explants, Organogenesis, Rhizogenesis, Saccharum

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Open Access

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Creative Commons Attribution 4.0 International License

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