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Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

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An efficient protocol for induction of callus and regeneration of a sugar cane var 93v297 has been developed and reported here. Callus induction from immature young leaf explants derived from 2-3-month-old plants was achieved on Murashige and Skoog’s (MS) medium supplemented with different auxins viz, 2,4-D, NAA and IAA. Among different auxins, 2, 4-D at 3.5mg/l + 0.5mg/l BAP was found favourable in inducing callus. Addition of coconut milk and BAP further enhanced the growth of callus maximum being on MS medium supplemented with 0.5mg/l BAP (3602.33±0.88mg). Calli were further evaluated for regeneration. MS medium supplemented with 1.0 mg/l BAP was found suitable where 100% calli regenerated with maximum number of multiple shoots per callus mass (41.40±0.89). Highest number of root emergence (28.33±1.16) and maximum root length (3.40±0.67cm) was achieved on MS medium supplemented with 3mgl/l NAA. The in vitro grown plants were transferred to polycups containing a mixture of sterilized sand, soil and cocopeet (1:1:1) for hardening. The hardened plants were transferred to green-house conditions where they survived with 90% frequency.


International Letters of Natural Sciences (Volume 48)
Arjun and R. Srinath, "Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297", International Letters of Natural Sciences, Vol. 48, pp. 14-22, 2015
Online since:
Nov 2015

[1] Ahloowalia, B.S. and Meretzki, A., Plant regeneration via somatic embryogenesis in sugarcane. Plant Cell Rep 2 (1983) 21-25.

[2] Alam, R., MafizurRahaman, A.B.M. and Gupta, S., In vitro plant regeneration from leaf sheath callus of sugarcane via organogenesis. Plant Cell Biotech. Mol. Biol. 3 (2003) 131-136.

[3] Alcantara G. B., Dibax R., Oliveira R. A. Filho J. C. B. and Daros E., Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp. ). Acta Scientiarum Vol. 36 (1) (2014) 63-72.

DOI: 10.4025/actasciagron.v36i1.16342

[4] Barba, R. and Nickel, I. G., Nutrition and organ differentiation in tissue cultures of sugarcane-a monocotyledon. Planta 89 (1969) 299-302.

DOI: 10.1007/bf00385034

[5] Behara, K.K. and Sahoo, S., Rapid in vitro micropropagation of sugarcane (Saccharum officinarum L. cv-Nayana) through callus culture. Nature and Science7 (2009) 1-10.

[6] Chatenet, M., Delage, C. and Ripolles, K. Detection of sugarcane yellow leaf curl virus in quarantine and production of virus-free sugarcane by apical meristem culture. Plant Disease 85 (2001) 1177-1180.

DOI: 10.1094/pdis.2001.85.11.1177

[7] Chengalarayan K. and Gallo-Meagher M. Effect of various growth regulators on shoot regeneration studies in sugarcane variety CO-86032. Ad Plant Sci 6 (2001) 649-651.

[8] Dibax R., Alcantra G. B., Filho J. C. B., Machado M. P., Oliveira Y. and Silva A. L. L., Plant regeneration of sugarcane cv. RB931003 and RB98710 from somatic embryos and acclimatization. Journal of Biotechnology and Biodiversity Vol. 2 (3) (2011).

[9] FAOSTAT, (2014). Food and Agriculture Organization Stitistics, accessed on (2015).

[10] Gallo-Meagher M., English R. G. And Abouzid A., Thidiazuran stiumaltes shoot regeneration of sugarcane embryogenic callus. In Vitro Cell Dev Biol- Plant 36 (2000) 37-40.

DOI: 10.1007/s11627-000-0009-3

[11] Gandonou C., Errabii T., Abrini J., Idaomar M., Chibi F. and Skalisenhaji N., Effect of genotype on callus induction and plant regeneration from leaf explants of sugarcane (Saccharum Sp. ). African J Biotech. 4 (2005) 1250-1255.

[12] Gill N. K., Gill R. and Gosal S. S., Factor enhancing somatic embryogenesis in sugarcane (Saccharum officinarum L. ). Indian Journal of Biotechnology. Vol. 3 (2004) 119-123.

[13] Gopitha K., Bhavani L. and Senthilmanickm J., Effect of different auxins and cytokinins in callus induction, shoot, root regeneration in sugarcane. Int J Pharma Bio Sci1 (2010) 1-7.

[14] Heinz D. L., Krishnamurthy G. and Nickell G., Cell tissue and organ culture in sugarcane improvement. In: Applied and fundamental aspects of Plant Cell, Tissue and Organ Cult. (Ed. ) Reinert J. and Balaji Y.P.S., Narosa publishing House, New Delhi, India. Pp 13-17. (1990).

DOI: 10.1007/978-3-662-02279-5

[15] Heinz D. J. and Mee G. W., Plant differentiation from callus tissues of Saccharum species. Crop Sci 9 (1969) 324-348.

[16] Ho W. And Vasil I. K., Somatic embryogenesis in sugarcane (Saccaharum officinarum L. ): Growth and plant regeneration from embryogenic cell suspension cultures. Ann Botany 51 (1983) 719-726.

DOI: 10.1093/oxfordjournals.aob.a086523

[17] Kaur A. and Gosal S. S., Desiccation of callus enhances somatic embryogenesis and subsequent shoot regeneration in sugarcane. Indian Journal of Biotechnology Vol. 8 (2009) 332-334.

[18] Lee T.S.G., Micropropagation of sugarcane (Saccharum spp. ) Plant Cell Tiss Org Cult 10 (1987) 47-55.

DOI: 10.1007/bf00037496

[19] Liu M. C., Factors affecting induction, somatic embryogenesis and plant regeneration of callus from cultured inflorescences of sugarcane. J Plant Physiol 141 (1993) 714-720.

DOI: 10.1016/s0176-1617(11)81580-4

[20] Maretzki A. Tissue culture: Its prospects and problems In: sugarcane improvement through breeding. (Ed. ) DJ Heinz. Elsevier Science Publication BV pp.343-384 (1987).

DOI: 10.1016/b978-0-444-42769-4.50014-x

[21] Matsuoka M., Ideta O., Tanio M., Hayakawa A. and Miwa H. Agrobacterium tumefaciens-mediated transformation of sugarcane using cell suspension culture with a novel method. Inter Soci Sugarcane Tech Proc of the XXIV Congress, Brisbane, Australia, 2 (2001).

[22] Meretzaki A. and Hiraki P. Sucrose promotion of root formation on plantlets regenerated from callus of Saccharum spp. Phyton 38 (1980) 85-88.

[23] Mittal Pallavi, Ruma Devi and S.S. Gosal, Direct Plant Regeneration from Spindle Leaf Roll Explants in Sugarcane. Indian J. Ecol. 40 (1) (2013) 67-70.

[24] Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15 (1962) 473-497.

DOI: 10.1111/j.1399-3054.1962.tb08052.x

[25] Naik G. R., Harinath Babu K. and Lingappa G., Studies on in vitro selection of Fe efficient lines in sugarcane. Plant and Soil 129 (1990) 183-186.

DOI: 10.1007/bf00032411

[26] Nandlal P., High frequency plant regeneration from sugarcane callus. Sugar Tech 5 (2003) 89-91.

DOI: 10.1007/bf02943773

[27] Rahulbaksha A. K. M., Alam-Ziaulkarim R., Mannan S. K., Mafizur-Rahaman A. B. M. and Gupta S., Anther culture and plant regeneration in sugarcane (Saccharum officinarum L. ). Plant Cell Biotech. Mol. Biol. 4 (2003) 179-184.

[28] Ramgareeb S., Snyman S. J., Van Antwerpen T. and Rutherford R. S., Elimination of virus and rapid propagation of disease free sugarcane (Saccharum spp. Cultivar NCo376) using apical meristem culture. Plant Cell Tiss Organ Cult. 100 (2010).

DOI: 10.1007/s11240-009-9634-7

[29] Rao S. and Jabeen F. T. Z. Optimization of Protocols for Callus Induction, Regeneration and Acclimatization of Sugarcane (Saccharum officinarum L. ) Cultivar CO-86032. Current trends in biotechnology and pharmacy 7 (4) (2013) 861-869.

[30] Schenck S. and Lehrer A. T. Factors affecting the transmission and spread of sugarcane yellow leaf virus. Plant Disease 84 (2000) 1085-1088.

DOI: 10.1094/pdis.2000.84.10.1085

[31] Soares R. R., Ferreira M. E., Gamarano M. C., Rebeiro R. C. Sabino V. M., and Pereira B. M. H., The use of histological analysis for the detection of somatic embryos in sugarcane. African Journal of Biotechnology Vol. 13 (6) (2014) 762-767.

DOI: 10.5897/ajb2013.12866

[32] Wamaitha M. J., Suwa K., Fukuda K., Mir M. Daimon H. and Mishiba K., Thidiazuran-induced rapid shoot regeneration via embryo like structure formation from shoot tip-derived callus cultures of Sugarcane. Plant Biotechnology 27 (2010) 365-368.

DOI: 10.5511/plantbiotechnology.27.365
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