LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

Mahmoudi, A.

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LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

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Abstract:

Development and validation of rapid HPLC method for quantifying macrolide antibiotics azithromycin (AZI) and spiramycin (SPI) in bulk and tablet samples is described. Determination was performed on a reversed phase C₁₈ ODB column (250×4.6 nm I.D) at ambient temperature, and employing a UV-detection set at 210 nm. The mobile phase consists of acetonitrile –2-methyl-2-propanol–hydrogenphosphate buffer, pH 6.2, with 1.8% triethylamine (32:8: up to 100, v/v/v), delivered at a flow-rate of 1.1 mL min^-1. The assay is linear in concentration ranges of: 0.004–4.8 and 0.0003–1.2 mg mL⁻¹ for azithromycin and spiramycin, respectively, with detection limit of 0.02% for SPI and 0.03% for AZI. Recovery experiments revealed recovery of 98.51–100.82%. The applicability of this method in stability assessment studies is evaluated.

Info:

Periodical:

International Letters of Natural Sciences (Volume 47)

Pages:

1-10

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.47.1

Citation:

A. Mahmoudi, "LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples", International Letters of Natural Sciences, Vol. 47, pp. 1-10, 2015

Online since:

September 2015

Authors:

A. Mahmoudi

Keywords:

Azithromycin, HPLC-UV, Spiramycin, Stability Study, Tablet Dosage Forms

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Open Access

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Creative Commons Attribution 4.0 International License

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Cited By:

[1] A. Mahmoudi, "Efficient and simple HPLC method for spiramycin determination in urine samples and in pharmaceutical tablets", Separation Science Plus, Vol. 1, p. 253, 2018

DOI: https://doi.org/10.1002/sscp.201800014