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Isolation and Identification of Vibrio sp. in the Hepatopancreas of Cultured White Pacific Shrimp (Litopenaeus vannamei)

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The Vibrio is the most common genera with crustaceans often causing various diseases in Aquaculture and significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was carried out, the isolation and identification of pathogenic bacterial flora were isolated from infected in hepatopancreas of vannamei. The SPDS Oceanic farm, RS Aqua farm of and Valli vilas Aqua farm Vellar estuary, Cuddalore District, Tamil Nadu, during the period of (September 2013 to November 2013). The collected samples were plated on TCBS- (Thiosulfate-Citrate-Bile salt-Sucrose) agar medium. The present study, totally 253 green colonies were isolated from TCBS agar plates and among these, 175colonies were identified by using the biochemical tests showed the V. parahaemolyticus, V. mimicus, V. vulnificus, V. damsela and P. shigelloides. The maximum species was recorded in V. parahaemolyticus (83.4 %) and minimum was observed in V. mimicus (1.7 %).


International Letters of Natural Sciences (Volume 46)
S. Shanmugasundaram et al., "Isolation and Identification of Vibrio sp. in the Hepatopancreas of Cultured White Pacific Shrimp (Litopenaeus vannamei)", International Letters of Natural Sciences, Vol. 46, pp. 52-59, 2015
Online since:
September 2015

Subasinghe R. D, Bartly M, Mcgladdery S, Barg U., Advances in shrimp biotechnology. National Centre for Genetic Engineering and Biotechnology, Bankok, (1998).

Nimrat Subuntith, Bart Amrit N, Keatsaksit Aritsara, Vuthiphandchai Verapong., Aquaculture, (2008) vol. 274, no. 2-4, pp.247-253.

FAO (Food & Agriculture Organization of the United Nations), the State of World Fisheries and Aquaculture (SOFIA). Rome: FAO, (2002).

Holthuis L.B., FAO Fish Synop. 125. Rome: FAO. (1980).

Kautsky N, Ronnback P, Tedengren M, Troell M., Aquaculture, (2000). 191, 145-161.

Lightner D. V, Bell T. A, Redman R. M, Mohney L. L, Natividad J. M, Rukyani A, Poernomo A., In Diseases in Asian Aquaculture, Fish Health Section, Asian Fisheries Society, (1992) p.5780.

Musa N, Wei L. S, Shaharom F, Wee W., World Applied Sciences Journal, (2008) 3(6): 903905.

Damsgard B, sorum U, Ugelstad I, Eliassen R. A, Mortensen A., Aquaculture, (2004) 239, 3746.

Baticados M.C. L, Lavilla-Pitogo C. R, Cruz-Lacierda E. R, de la Pena L. D, Sunaz. N.A., Dis. Aquat. Organ, (1990) 9: 133-139.

Mohney L. L, Lightner D. V, Bell T.A., J world Aquaculr, (1994) 25: 116-125.

Jolt J. G, Krieg N. R, Stanley J. T, Williams S.T., Bergey's manual of systematic bacteriology. 9th edn. Maryland: Williams and wilkins Co. Baltimore. (1994) 786.

Jayasree L, Janakiram P, Madhavi R., J. World Aquac. Soc, (2006) 37: 523-532.

Raghavan R.P., Naga World Fish Center Quarterly, (2003) 26: 22-24.

Ni C, Lin Y, Ye D, Zeng H, Yao R, Gu J., J. Oceanogr. Taiwan Straits/Taiwan Haixia, (1995) 14: 73-79.

Dalsgaard A, Serichantalergs O, Shimada T, Sethabutr O, Echeverria P., J. Med. Microbiol, (1995) 43: 216-220.

Tendencia A.E. V, Dureza L.A., Aquaculture, (1997) 154: 107-114.

Li Q, Chen B, Qu K, Xin F, Li J, Zhao F, Yan Y., J. Fish. Sci. China, (2002) 9: 367-370.

Wei W. C, Hsu C.H., 6th Asian Fisheries Forum Book of Abstracts, (2001) p.346, 25-30.

Liu C.I., Fish diseases Proc. R. Jpn. Symp. Taipei, Taiwan, (1990) pp.180-201.

Brock J. A, Lea Master B., World Aquaculture Society, Baton Rouge, Louisiana, USA, (1992) pp.212-226.

Aguirre Guzman G., Programa Nacional de Sanidad Acuicola y la Red de Diagnostico, (2004) 1: 1-9.

Chen S. N, Huang S. L, Kou G.N., The oceanic institute, (1992) 195-206.

Olsen J. E, Aabo S, Hill W, Notermans S, Wernars K, Granum P. E, Popovic T, Rasmussen H. N, Olsvik O., Inst.J. Food Microbiol, (1995) 28: 1-78.

Lavilla Pitogo C. R, Leano E. M, Paner M.G., Aquaculture, (1998) 164: 337-349.

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[1] S. Muthukrishnan, T. Defoirdt, M. Ina-Salwany, F. Yusoff, M. Shariff, S. Ismail, I. Natrah, "Vibrio parahaemolyticus and Vibrio harveyi causing Acute Hepatopancreatic Necrosis Disease (AHPND) in Penaeus vannamei (Boone, 1931) isolated from Malaysian shrimp ponds", Aquaculture, p. 734227, 2019


[2] N. Estrada‐Perez, J. Ruiz‐Velazco, F. Magallon‐Barajas, A. Campa‐Cordova, A. Hernández‐Llamas, " Dynamic stock model for analysing semi‐intensive production of whiteleg shrimp Litopenaeus ( Penaeus ) vannamei affected by the acute hepatopancreatic necrosis disease: Assessment of disease severity indicators and relationships with pond water quality parameters ", Aquaculture Research, 2019