Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > Volume 46 > Antibacterial Activity of Extracts of Leaves of...
< Back to Volume

Antibacterial Activity of Extracts of Leaves of Nerium indicum in Combination with Antibiotics

Full Text PDF


Methanol and ethanol extract showed antibacterial activity in range of 0-11 at 10 mg/mL at against all tested bacterial species. Methanol extract with amoxicillin exhibited effective inhibition E. coli. This combination showed antagonistic effect against B. subtilis and S. aureus. Interaction of ethanol extract with amoxicillin also showed effective inhibition against E. coli and decreased activity against B. subtilis and S. aureus.Methanol extract did not show inhibition against P. aeruginosa but it enhanced activity of ciprofloxacin on combination. Ethanol extract also exhibited effective inhibition against P. aeruginosa on combination with ciprofloxacin. But antagonist effect was observed on interaction of both extract with ciprofloxacin against other bacterial species.Interaction of methanol and ceftazidime showed mostly decreased or indifferent effect against all bacterial species. Ethanol extract with ceftazidime resulted in higher inhibition against E. coli. Combination of methanol extract with erythromycin showed higher inhibition against E. coli and also very effective inhibition against S. aureus. This combination resulted in decreased activity against B. subtilis. Ethanol extract with erythromycin also showed synergistic effect against S. aureus and effective inhibition against E. coli. These results indicate that both methanol and ethanol extract has potential to enhance the combination effect with erythromycin against S. aureus and E. coli.


International Letters of Natural Sciences (Volume 46)
P. Vyas et al., "Antibacterial Activity of Extracts of Leaves of Nerium indicum in Combination with Antibiotics", International Letters of Natural Sciences, Vol. 46, pp. 41-45, 2015
Online since:
Sep 2015

Samy, R. P., Ignacimuthu, S., & Raja, D. P. (1999). Preliminary screening of ethnomedicinal plants from India. Journal of Ethnopharmacology, 66(2), 235-240.

Abreu, A. C., McBain, A. J., & Simoes, M. (2012). Plants as sources of new antimicrobials and resistance-modifying agents. Natural product reports, 29(9), 1007-1021.

Hemaiswarya, S., Kruthiventi, A. K., & Doble, M. (2008). Synergism between natural products and antibiotics against infectious diseases. Phytomedicine, 15(8), 639-652.

Chatterjee, S. K., Bhattacharjee, I., & Chandra, G. (2009). In vitro synergistic effect of doxycycline & ofloxacin in combination with ethanolic leaf extract of Vangueria spinosa against four pathogenic bacteria. Indian J Med Res, 130, 475-478.

Aiyegoro, O., Adewusi, A., Oyedemi, S., Akinpelu, D., & Okoh, A. (2011).

Purushotham, K. G., Arun, P., Jayarani, J. J., Vasnthakumari, R., Sankar, L., & Reddy, B. R. (2010). Synergistic in vitro antibacterial activity of Tectona grandis leaves with tetracycline. Int J PharmTech Res, 2, 519-523.

Sharma, B., Fatima, A., & Agarwa, P. (2013). A Review: Phytochemistry and Pharmacological Potential of Nerium indicum Mill, 3(1), 39-45.

Jawarkar, A.G., Shrirao, A.V., Mohale, D.S., Chandewar , A.V., Marathe, S.J., & Mahajan, P.G. . (2012). Brief Review on Medicinal Potential of Nerium indicum, International Journal of Institutional Pharmacy and Life Sciences, 2(2), 521-527.

http: /www. spicesmedicinalherbs. com/nerium-indicum. html.

http: /www. motherherbs. com/nerium-indicum. html.

Reddy, B. U. (2010). Antimicrobial Activity of Thevetia Peruviana (Pers. ) K. Schum. and Nerium Indicum Linn. Internet Journal of Pharmacology, 8(2), 2.

Vinayagam, A., & Sudha, P. N. (2011). Antioxidant activity of methanolic extracts of leaves and flowers of Nerium indicum. Int. J. Pharm. Sci. Res, 2, 1548-1553.

Shah, A., Zambare, A. V., Desai, A., & Chakraborthy, G. S. BIOLOGICAL EVALUATIONS OF POTENTIAL HERB-NERIUM INDICUM (LINN. ). International Journal of Pharmaceutical Sciences and Research, 2(2), 418-423.

Rajbhandari, M., Wegner, U., Jülich, M., Schoepke, T., & Mentel, R. (2001). Screening of Nepalese medicinal plants for antiviral activity. Journal of Ethnopharmacology, 74(3), 251-255.

Sharma, B., Fatima, A., & Agarwa, P. (2013). A Review: Phytochemistry and Pharmacological Potential of Nerium indicum Mill, 3(1), 39-45.

http: /www. mpbd. info/plants/nerium-indicum. php.

Kaur, S. P., Rao, R., & Nanda, S. (2011). AMOXICILLIN: A BROAD SPECTRUM ANTIBIOTIC. International Journal of Pharmacy & Pharmaceutical Sciences, 3(3).

Adikwu, E., & Brambaifa, N. (2012). Ciprofloxacin Cardiotoxicity and Hepatotoxicity in Humans and Animals. Pharmacology & Pharmacy, 3(2).

Gay, J. D., DeYoung, D. R., & Roberts, G. D. (1984).

http: /en. wikipedia. org/wiki/Erythromycin.

Savoia, D. (2012). Plant-derived antimicrobial compounds: alternatives to antibiotics. Future microbiology, 7(8), 979-990.

Sibanda, T., & Okoh, A. I. (2007). The challenges of overcoming antibiotic resistance: Plant extracts as potential sources of antimicrobial and resistance modifying agents. African Journal of Biotechnology, 6(25), 2886-2896.

Stefanovic, O., & Comic, L. (2012). Synergistic antibacterial interaction between Melissa officinalis extracts and antibiotics. Journal of Applied Pharmaceutical Science, 02 (01), 01-05.

Show More Hide