Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > Volume 43 > Enhanced Production, Purification and...
< Back to Volume

Enhanced Production, Purification and Characterization of Alkaline Keratinase from Streptomyces minutiscleroticus DNA38

Full Text PDF

Abstract:

A thermo tolerant, feather-degrading, newly isolated actinobacterial strain Streptomyces minutiscleroticus DNA38 was investigated for its ability to produce keratinase. Maximum production (283.4 IU) of keratinase by Streptomyces minutiscleroticus DNA38 in starch chicken feathers medium under submerged bioprocess was observed at optimized conditions of pH 9.0 of the medium and 45 °C incubation temperature. Further, an enhanced production (435.8 IU) of keratinase was achieved employing response surface methodology. Combined interactive effect of starch (7.50 g/L), yeast extract (0.74 g/L) and chicken feathers (7.50 g/L) were found to be the critical process variables for enhanced production under central composite design. Chicken feathers showed a direct action and addition of starch and yeast extract to the medium proved effective for a significant increase in the production of keratinase. The purified keratinase was monomeric and had a molecular mass of 29 kDa. The enzyme activity was significantly inhibited after pH 9.0 and temperature 50 °C.

Info:

Periodical:
International Letters of Natural Sciences (Volume 43)
Pages:
27-37
Citation:
A. Nandini et al., "Enhanced Production, Purification and Characterization of Alkaline Keratinase from Streptomyces minutiscleroticus DNA38", International Letters of Natural Sciences, Vol. 43, pp. 27-37, 2015
Online since:
July 2015
Export:
Distribution:
References:

[1] D.R. Goddard, L. Michaels, A study of keratin, J. Biol Chem. 106 (1934) 604-614.

[2] M.C. Papadopoulos, The effect of enzymatic treatment on amino acid content and nitrogen characteristics of feather meal, Anim. Feed Sci. Technol. 16 (1986) 151-156.

DOI: https://doi.org/10.1016/0377-8401(86)90058-1

[3] J. Ryckeboer, J. Mergaert, J. Coosemans, K. Deprins, J. Swings, Microbiological aspects of biowaste during composting in a monitored compost bin. Journal of Applied Microbiology. 94 (2003) 127-137.

DOI: https://doi.org/10.1046/j.1365-2672.2003.01800.x

[4] M.B. Rao, A.M. Tanksale, M.S. Ghatge, V.V. Deshpande, Molecular and biotechnological aspects of microbial proteases, Microbiology and MolecularBiology Reviews. 62 (1998) 597-635.

[5] X. Lin, C.G. Lee, E.S. Casale, J.C.H. Shih, Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Strain, Applied and Environmental Microbiology. 58 (1992) 3271-3275.

[6] A.A. Raju, N.K. Chandrababu, N. Samivelu, C. Rose, N.M. Rao, Eco-friendly enzymatic dehairing using extracellular proteases from Bacillus species isolate, Journal of the American Leather Chemical Association. 91 (1996) 115-119.

[7] J.J. Wang, J.C.H. Shih, Fermentation production of keratinase from Bacillus licheniformis PWD-1 and a recombinant B. subtilis FDB-29, Journal of Industrial Microbiology and Biotechnology. 22 (1999) 608-616.

DOI: https://doi.org/10.1038/sj.jim.2900667

[8] Z. Ignatova, A. Gousterova, G. Spassov, P. Nedkov, Isolation and partial characterisation of extracellular keratinase from a wool degrading thermophilic actinomycete strain Thermoactinomyces candidus. Canadian Journal of Microbiology 45 (1999).

DOI: https://doi.org/10.1139/cjm-45-3-217

[9] P Kabadjova, S. Vlahov, M. Dalgalarrondo, X. Dousset, T. Haertle, L. Briand, J.M. Chobert, Isolation and distribution of Streptomyces populations with heat-resistant collagenolytic activity from two protein-rich areasin Bulgarian soils. Folia Microbiologica. 41 (1996).

DOI: https://doi.org/10.1007/bf02815693

[10] M. Han, W. Luo, Q. Gu, X. Yu, Isolation and characterization of a keratinolytic protease from a feather-degrading bacterium Pseudomonas aeruginosa C11, African Journal of Microbiology Research. 6 (2012) 2211–2222.

DOI: https://doi.org/10.5897/ajmr11.921

[11] B. Deivasigamani, K.M. Alagappan, Industrial application of keratinase and soluble proteins from feather keratins, Journal of Environmental Biology. 29 (2008) 933–936.

[12] R.P. Mukhopadhyay, A.L. Chandra, Protease of a keratinolytic streptomycete to unhair goat skin, Indian Journal of Experimental Biology. 31 (1993) 557–558.

[13] G.W. Nam, D.W. Lee, H.S. Lee et al., Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe, Archives of Microbiology. 178 (2002) 538–547.

DOI: https://doi.org/10.1007/s00203-002-0489-0

[14] Suneetha V., V.V. Lakshmi, Optimisation of parameters for fermentative production of keratinase by feather degrading microorganisms, World Journal of Microbiology and Biotechnology. 7 (2005) 106–115.

[15] R. Tatineni, K.K. Doddapaneni, R.C. Potumarthi et al., Purification and characterization of an alkaline keratinase from Streptomyces sp., Bioresource Technology, 99 (2008) 1596–1602.

DOI: https://doi.org/10.1016/j.biortech.2007.04.019

[16] L. Kumar, B. Singh, K.D. Adhikari, J. Mutherjee, D. Ghosh, A temperature and salt tolerant L-glutaminase from Gangotri region of Uttarakhand Himalaya: enzyme purification and characterization. Appl. Biochem. Biotechnol. 166 (2012) 1723-1735.

DOI: https://doi.org/10.1007/s12010-012-9576-0

[17] G.S. Dastager, J. Chan Lee, L. Wen – Jun, K. Chang – Jin, A. Dayanand, Production, characterization and application of keratinase from Streptomyces gulbargensis. Bioresour. Technol. 100 (2009) 1868-1871.

DOI: https://doi.org/10.1016/j.biortech.2008.09.047

[18] Y. Liuan, G. Su, B. Zhang, G. Jiang, B. Yan, Nanoparticle-based strategies for detection and remediation of environmental pollutants, Journal The Royal Society of Chemistry (2010).

[19] S.W. Cheng, H.M. Hu, S.W. Shen, H. Takagi, M. Asano, Y.C. Tsai, Production and characterization of keratinase of a feather- degrading Bacillus licheniformis PWD-1. Biosci. Biotechnol. Biochem. 59 (1995) 2239-2243.

[20] Annapurna Kumari, Paramita Mahapatra, Rintu Banerjee, Statistical Optimization of Culture Conditions by Response Surface Methodology for Synthesis of Lipase with Enterobacter aerogenes, Braz. Arch. Biol. Technol, 52 (2009) 1349-1356.

DOI: https://doi.org/10.1590/s1516-89132009000600005

[21] R. Wenk, Fernandis Aaron Zefrin, Manuals in Biomedical Rsearch – Vol. 3. A manual for Biochemistry Protocols. Jan – Thorsten Schantz (Eds. ). World Scientific publishing Co. Pte. Ltd. Singapore. (2007).

DOI: https://doi.org/10.1142/9789812708311

[22] O.H. Lowry, N.J. Rosenbrough, A.L. Farr, R.J. Randal, Protein measurement with Folin-phenol reagent, J. Biol. Chem. 193 (1951) 265-75.

[23] U.K. Laemmli, Cleavage of structural proteins during the assembly of the bacteriophage t4. Nature. 227 (1970) 680-685.

DOI: https://doi.org/10.1038/227680a0

[24] N. Liu, T. Zhang, Y.J. Wang, Y.P. Huang, J.H. Ou, P. Shen, A heat inducible tyrosinase with distinct properties from Bacillus thuringiensis. Letters in Applied Microbiology. 39 (2001) 407-412.

DOI: https://doi.org/10.1111/j.1472-765x.2004.01599.x

[25] P.D. Haaland (Ed. ) Separating Signals from the Noise. In: Experimental Design in Biotechnology. Marcel Dekker, New York, 1989, pp.61-83.

[26] P. Jayalakshmi, G. Krishnamoorthy, P. Ramesh kumar, Sivamani, Optimization of culture conditions for Keratinase production in Streptomyces sp. JRS19 for Chick feather wastes degradation, J. Chem. Pharm. Res., 3 (2011) 498-503.

[27] Shilpa Ashok Jani, Raval Heta, Harnisha Patel, Drashti Darji, Ankit Rathod, Seema Pal, Production and characterization of keratinolytic protease from Streptomyces sp., Int. J. Curr. Microbiol. App. Sci. 4 (2015) 962-975.

[28] Ana Maria Mazotto, Rosalie Reed Rodrigues Coelho, Sabrina Martins Lage Cedrola, et al., Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation, Enzyme Research. Article ID 523780, 7 pages. doi: 10. 4061/2011/523780. (2011).

DOI: https://doi.org/10.4061/2011/523780

[29] Li-Jung Yin, Jein-Hwa Lee, Shann-Tzong Jiang, Isolation of a Keratinase-Producing Bacterium and purification of its keratinase, J. Fish. Soc. Taiwan. 33 (2006) 377-390.

[30] Xiang Lin, Chung-Ginn Lee, S. Ellen Casale, C.H. Jason Shih, Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Strain, Applied and Environmental Microbiology. (1992) 3271-3275.

[31] Brigitte Bockle, Boris Galunsky and Rudolf Muller, Characterization of a Keratinolytic Serine Proteinase from Streptomyces pactum DSM 40530, Applied and Environmental Microbiology. (Oct. 1995) 3705–3710.

[32] C.A.I. Cheng-Gang, Ji-Shuang Chen, Q.I. Jiong-Jiong, Yun Yin, Xiao-Dong Zheng. Purification and characterization of keratinase from a new Bacillus subtilis strain, J. Zhejiang Univ. Sci. B. 9 (2008) 713-720.

DOI: https://doi.org/10.1631/jzus.b0820128

[33] S.K. Pushpalata, G.R. Naik, Production and characterization of feather degrading keratinase from Bacillus sp. JB 99, Indian Journal of Biotechnology. 9 (2010) 384-390.

[34] Venkata Saibabu, Francois Niyongabo Niyonzima and S. Sunil More, Isolation, Partial purification and Characterization of Keratinase from Bacillus megaterium, Int. Res. J. Biological Sci. 2 (2013) 13-20.

[35] Sreenivasa Nayaka, K Gireesh Babu, G.M. Vidyasagar, Purification and characterization of keratinase from hair-degrading streptomyces albus. International Journal of Bioassays. 2 (03) (2013), 599-604.

Show More Hide
Cited By:

[1] M. Călin, D. Constantinescu-Aruxandei, E. Alexandrescu, I. Răut, M. Doni, M. Arsene, F. Oancea, L. Jecu, V. Lazăr, "Degradation of keratin substrates by keratinolytic fungi", Electronic Journal of Biotechnology, Vol. 28, p. 101, 2017

DOI: https://doi.org/10.1016/j.ejbt.2017.05.007