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Antioxidant and Free Radical Scavenging Effect of D-Carvone in Hypertensive Rats In Vivo and In Vitro Study

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This study was planned to assess the antioxidant and free radical scavenging effect of D-carvone against L-NAME (Nω-nitro-L-arginine methyl ester hydrochloride) induced hypertension. Hypertension was encouraged in adult male albino rats of the Wistar strain, considering 180–230 g, by oral administration of the L-NAME (40 mg/kg/ body weight/day) in drinking water for 4 weeks. Rats were cured with D-carvone (5, 10 and 20 mg/kg body weight) for four weeks. A significant reduction in the levels of non-enzymatic antioxidants such as vitamin C, vitamin E and reduced glutathione (GSH), in plasma were perceived in L-NAME induced hypertensive rats. Moreover, in vitro free radical scavenging activity of ABTS+ and DPPH radical scavenging possible of D-carvone was also quantified. Treatment with D-carvone (5, 10 and 20 mg/kg bw) carries back all the above parameters to near usual level, in which 20 mg/kg displayed the highest effect than that of other two doses. Further, D-carvone displays concentration dependent antioxidant potential. These results suggest that D-carvone acts as an antioxidant and free radical scavenging agent against L-NAME induced hypertension.


International Letters of Natural Sciences (Volume 35)
T. Rajeshwari and B. Raja, "Antioxidant and Free Radical Scavenging Effect of D-Carvone in Hypertensive Rats In Vivo and In Vitro Study", International Letters of Natural Sciences, Vol. 35, pp. 6-12, 2015
Online since:
March 2015

Ceriello A. Possible role of oxidative stress in the pathogenesis of hypertension. Diabetes Care. 31(2008) 181-184.

Cutler JA, Sorlie PD, Wolz M, Thom T, Fields LE, Roccella EJ. Trends in hypertension prevalence, awareness, treatment, and control rates in United States adults between 1988-1994 and 1999-2004. Hypertension. 52 (2008) 818-827.

Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J. Global burden of hypertension: analysis of worldwide data. Lancet. 365 (2005) 217-223.

Higashi Y, Noma K, Yoshizumi M, Kihara Y. Endothelial function and oxidative stress in cardiovascular diseases. Circ. J. 73 (2009) 411-8.

Harrison DG. Cellular and molecular mechanisms of endothelial cell dysfunction. Journal of Clinical Investigation. 100 (1997) 2153-2157.

Olayinka A, Aiyegoro A, Okoh I. Preliminary phytochemical screening phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine. (2010) 10: 21.

Valko M, Rhodes C J, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interaction. (2006) 160: 1-40.

Mukherjee PK. Evaluation of Indian traditional medicine. Drug. Information. J. 35 (2001) 623-32.

Gorinstein S, Leontowicz H, Leontowicz M, Krzeminski R, Gralak M, Delgado Licon et al. Changes in plasma lipid and antioxidant activity in rats as a result of naringin and red grapefruit supplementation. Agric. Food. Chem. 53 (2005) 3223-3228.

Johri RK. Cuminumcyminum and Carumcarvi: An update. Pharmacognosy Reviews. 5 (2011) 63-72.

Vinothkumar R, Sudha M , Viswanathan P, Kabalimoorthy J, Balasubramanian T, Nalini N. Modulating effect of D-carvone on 1, 2-dimethylhydrazine induced preneoplastic lesions, oxidative stress and biotransforming enzymes in an experimental model of rat colon carcinogenesis. Cell Proliferation. 46 (2013).

Saravanakumar M, Raja B. Veratric acid, a phenolic acid attenuates blood pressure and oxidative stress in L-NAME induced hypertensive rats. Eur. J. Pharmacol. 671 (2011) 87-94.

Roe JH, Kuether CA. The determination of ascorbic acid in whole blood and urine through the 2, 4 -dinitrophenylhydrazine derivative of dehydroascorbic acid. J. Biol. Chem. 11 (1943) 145-164.

Baker H, Frank O, DeAngelis B. Plasma tocopherol in man at various times after ingesting free or acetylated tocopherol. Nutr. Res. 21 (1980) 531-536.

Ellman GL. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82 (1959) 70-77.

Miller NJ, Castelluccio C, Tijburg L, Rice-Evans C. The antioxidant properties of the flavins and their gallate esters radical scavengers or metal chelators. FEBS Letters. 392 (1996) 40-44.

Mensor LL, Menezes FS, Leitao GG, Reis AS, Dos santos TC, Coube CS, Leitao SG. Screening of Brazilian plant extracts antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 15 (2001) 127-130.

Mahdi AA. Free radicals and other antioxidants. A textbook of biochemistry by S.P. Singh, third ed. CBS, New Delhi. (2002) s545-555.

Roy AKD, Gordon MJ, Campbell FM, Duthie GG, James WPT. Vitamin-E requirement, transport and metabolism: role of α-tocopherol-binding protein. J. Nutr. Biochem. 5 (1994) 562-70.

Kitts DD, Yuan YV, Godin DV. Plasma and lipoprotein lipid composition and hepatic antioxidant status in spontaneously hypertensive (SHR) and normotensive (WKY) rats. Canadian Journal of Physiology and Pharmacology. 76 (1998) 202-209.

Chang T, Wu L. Methylglyoxal, oxidative stress and hypertension. Canadian Journal of Physiology and Pharmacology. 84 (2006) 1229-38. ( Received 02 February 2015; accepted 11 February 2015 ).

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