Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Chick Pea (Cicer arietinum L.) Var. Co - 4

Umavathi, S.; Mullainathan, L.

doi:10.18052/www.scipress.com/ILNS.35.1

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Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Chick Pea (Cicer arietinum L.) Var. Co - 4

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Abstract:

The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD₅₀). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M₁ generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD₅₀ values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.

Info:

Periodical:

International Letters of Natural Sciences (Volume 35)

Pages:

1-5

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.35.1

Citation:

S. Umavathi and L. Mullainathan, "Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Chick Pea (Cicer arietinum L.) Var. Co - 4", International Letters of Natural Sciences, Vol. 35, pp. 1-5, 2015

Online since:

March 2015

Authors:

S. Umavathi, L. Mullainathan

Keywords:

Germination Percentage, LD₅₀, Root Length, Seedling Height

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Open Access

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Creative Commons Attribution 4.0 International License

References:

Markeen Kouser G. Suresh Babu and Lavanya G.R. (2007); Effect of mutagenesis on M1 population in urdbean; Journal of Food Legumes, 20(1): 109-110.

Sax K. (1963); The Stimulation of Plant growth by ionizing; Rad. Bot., 3: 179- 186.

Ganesan, J. 1998. Induced mutation for Sesame improvement. Proc. Third FAO / IAEA Res. Co-ord. Meet. On induced mutations for Sesame improvement. 6-10 April, Bangkok, Thailand.

Kumar, A. and M.N. Mishra. 2004. Effect of gamma- rays EMS and NUM on germination, seedling Vigour, pollen viability and plant survival in M1 and M2 generation of Okra (Ablemoschus esculentus (L. ) Moench). Adv. Pl. Sci., 17 (1): 295-297.

Athwal, D. S 1963. Some X-rays induced spontaneous mutation in cicer. Indian. J. Genet., 23: 50-57.

Chaturvedi, S.N. and Paliwal, S.P. 1982. Effect of gamma rays, Ethyle Methane Sulphnonate and N-nitroso-N-Methyl urea on M, Parameters of Cajanus cajan (L. ) Mill sp. Sci. & cult., 48: 172-174.

Sharma, B. A., 1969: Non-random induction of multimutations in higher plants. Proceed. of the Symp. on Radiations and Radiomimetic Substances in Mutation Breeding, DAE/BARC, Mumbai, India. pp.22-31.

Dhakshanamoorthy, D., R. Selvaraj, and A. Chidambaram, 2010: Physical and chemical mutagenesis in Jatropha curcas L. to induce variability in seed germination, growth and yield traits. Rom. J. Biol. -Plant Biol. 55(2), 113-125.

Dube, K. G., A. S. Bajaj, and A. M. Gawande, 2011: Effect of gamma rays and ethylmethane sulphonate on germination, seedling height and survival in Cyamopsis tetragonoloba L. variety Sharada. Asiatic J. Biotech. Resour. 2(6), 747-754.

Sagade, A. B., and B. J. Apparao, 2011: M1 generation studies in urdbean (Vigna mungo (L. ) Hepper). Asian J. Exp. Biol. Sci. 2(2), 372-375.

Ananthaswamy, H. N., U. K. Vakil, and A. Srinivasan, 1971: Biochemical and physiological changes in gamma irradiated wheat during germination. Rad. Bot. 11, 1-12.

J. D. Barshile, International Letters of Natural Sciences 3 (2015) 1-10. ( Received 30 January 2015; accepted 08 February 2015 ).

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[1] F. Kusmiyati, . Sutarno, M. Sas, B. Herwibawa, "Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings", IOP Conference Series: Earth and Environmental Science, Vol. 102, p. 012059, 2018

DOI: https://doi.org/10.1088/1755-1315/102/1/012059