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ILNS > Volume 32 > Isolation and Screening of Lactobacillus Bacteria...
Isolation and Screening of <i>Lactobacillus</i> Bacteria for Ability to Produce Antibiotics
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Isolation and Screening of Lactobacillus Bacteria for Ability to Produce Antibiotics

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Abstract:

Antibiotic is one of the important commercially exploited secondary metabolites produced by bacteria and used in a wide range. Most of the antibiotics used today are isolated from the microbes. Bacteria are easy to culture, isolate, maintain and to improve their strain. Isolation of lactic acid bacteria (LAB) from soil, yoghurt and cheese was carried out. LAB were cultivated on De Man Rogosa Sharpe (MRS) agar and were characterized based on colony morphology, cell shape and biochemical tests. Out of eight samples analyzed, 96 isolates were identified as LAB. The bacterial isolates were identified as Lactococcus Lactis, Lactobacillus Brevis, Lactobacillus casei, Pediococcus damnosus, Lactobaciilus rhamnosus, Lactobacillus Plantarum, Lactobacillus pentosus, Enterococcus feacalis, Staphylococcus Simulans.Using MRS broth, the isolated LAB were screened for production of bacteriocins. Further, 96 LAB screened for bacteriocin production, 12 LAB were identified as bacteriocin producers. Out of 12 LAB, Enterococcus faecium (CST-1) was identified as potential bacteriocin producer against Bacillus subtlis MTCC-10403 Pseudomonas aureginosa MTCC-4676 microorganisms used while Pediococcus damnosus had the least bacteriocin activity against Staphylococcus aureus and Bacillus subtilis.

Info:

Periodical:
International Letters of Natural Sciences (Volume 32)
Pages:
68-76
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.32.68
Citation:
C. M. Lalam et al., "Isolation and Screening of Lactobacillus Bacteria for Ability to Produce Antibiotics", International Letters of Natural Sciences, Vol. 32, pp. 68-76, 2015
Online since:
January 2015
Authors:
Chandra Mouli Lalam, P.Y. Naidu, T. Srinivasan
Keywords:
Bacillus subtilis, Bacteriocin Activity, Pediococcus damnosus, Pseudomonas aureginosa, Staphylococcus Aureus
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Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] Carol AR, Leon MTD. 2010. Horizontal gene transfer amongst probiotic lactic acid bacteria and other intestinal microbiota: what are the possibilities? A review. Archives of Microbiology 193(3), 157-168.

DOI: https://doi.org/10.1007/s00203-010-0668-3

[2] Leroy F, De Vuyst L: Lactic acid bacteria asfunctional starter cultures for the food fermentation industry. Trends Food Sci Technol 2004; 15: 67–78.

DOI: https://doi.org/10.1016/j.tifs.2003.09.004

[3] Stiles, M.E., W.H. Holzapfel. 1997. Review article: Lactic acid bacteria of foods and their current taxonomy. Int. J. Food Microbiol. 36, 1-29.

[4] Jay, J.M. 2000. Fermentation and fermented dairy products, pp.113-130. In Modern Food Microbiology, 6th edition. An Aspen Publication, Aspen Publishers, Inc. Gaithersburg, USA.

DOI: https://doi.org/10.1007/978-1-4615-4427-2_7

[5] Holzapfel, W.H., P. Haberer, R. Geisen, J. Björkroth, U. Schillinger. 2001. Taxonomy and important features of probiotic microorganisms in food nutrition. Am. J. Clin. Nutr. 73, 365S-373S.

[6] Cleveland J, Montiville TJ, Nes I F & Chikindas M L, Bacteriocins: Safe, natural antimicrobials for food preservation, Int. J Food Microbial, 71(2001)1-20.

DOI: https://doi.org/10.1016/s0168-1605(01)00560-8

[7] Michaela S, Reinhard W, Gerhard K, Christine M-E. 2009. Cultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms. Applied and Environmental Microbiology 11(75), 3484-3491.

DOI: https://doi.org/10.1128/aem.02565-08

[8] Berg, R., 1996. The indigenous gastrointestinal microflora. Trends Microbiol., 4: 430-435.

[9] Oberg, C.J., J.R. Broadbent and D.J. McMahon, 1998. Applications of EPS production by LAB. J. Appl. Microbiol., 150: 1187-1193. ( Received 26 December 2014; accepted 11 January 2015 ).

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