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Immobilization of Pseudomonas sp. KLM9 in Sodium Alginate: A Promising Technique for L-Glutaminase Production

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Microbes and process development are gaining considerable importance owing to their exploitation and suitability for the production of the enzymes of therapeutic and industrial significance. In the present study attempts were made to immobilize the Pseudomonas Sp. KLM9 in different supporting matrix to analyze the possible enhancement in the production of L-Glutaminase. The consequences of the study reveal that, sodium alginate at 3.5% concentration was found to be the better supporting matrix than the others. Further, initial pH 7 of the incubation media, incubation temperature of 37°C were supported good yield of L-Glutaminase by immobilized Pseudomonas Sp KLM9. The fermentation period was reduced to 36 hr with immobilized cells. There is moderate increase in the production L-Glutaminase by the immobilized cells than the free cells of Pseudomonas Sp KLM9. Biomass obtained from the 18 hr old culture of Pseudomonas Sp KLM9 was found better to immobilize for the production of L-Glutaminase.


International Letters of Natural Sciences (Volume 31)
D. Mahesh et al., "Immobilization of Pseudomonas sp. KLM9 in Sodium Alginate: A Promising Technique for L-Glutaminase Production", International Letters of Natural Sciences, Vol. 31, pp. 27-35, 2015
Online since:
January 2015

Farid A. El-asmar and David M. Greenberg. Studies on the Mechanism of Inhibition of Tumor Growth by the Enzyme Glutaminase. Cancer Research 26, 16-12. (1966).

Wiley W Soub. Glutamine and cancer. Annals of Surgery. (1993).

J Roberts and W G McGregor. Inhibition of mouse retroviral disease by bioactive glutaminase-asparaginase. Journal of General Virology. 72 ( Pt 2): 299-305. (1991).

Alexandra Weingand, Christane Gerber-Decombaz, Michael Affolter. Functional Characterization of a salt and Thermotallerant glutaminase from Lactobacillus rhamnosus. Enzyne and Microbial Technology. 32, 862-867. (2003).

S V Ramakrishna and S Prakasham. Microbial Fermentation with Immobilized cells. Current science. 77, 1-10. (1999).

S Rajeev Kumar and m chandrashekaran. Continuos Production of L-Glutaminase by an immobilized marime Pseudomonas sp BTMS-51 in Packed Bed reactor. Process Biochemistry. 38, 1431-1436. (2002).

S Sankarlingam., T Shankar., K SendeskannanR Ramasubburayan and S Prakash. Europ. J. Appl. Sci. 4 (4), 146-156. (2012).

Kuo-Ying-Amada Wu and Keith D Wisecaver. Cell immobilization Using PVA crossed linked with Boric acid. Biotechnology and Bioengineering. 39(4). (1991).

Zhang Li Sheng., WuWei-Zhong and Wang Jian Long. Immobilization of Activated Sludge Using improved polyvinyl Alcohol (PVA) Gel. Journal of environmental Science. 19, 1293-1297. (2007).

Ajao A T, Adebayo G b and Yakubu S E. Bioremidiation of Textile Industrial Effluents Using mixed Culture of Pseudomonas arugenosa and Bacillus subtilis. J. Micribiol. Res. , 1 (3), 50-56. (2011).

Pramod T and Lingappa K. Immobilization of Aspergillus niger in Henn White for the Production of citric acid using Carob pod extract. J. Micribiol. Res., 2 (2), 265-269. (2012).

Amena S, Vishalaxi N, Prabhakar M and Lingappa K. Production Purification and Characterization of L-Asperginase Streptomyces gulbergensis. Brazilian journal of Microbiology., 41. 173-178. (2010).

Mahesh Divatar, Prabhakar M and Lingappa K. Evaluation of Bioprocess, Raw materials and Conditions for the Production of L-Glutaminase by Pseudomonas Sp. KLM9. Asian Journal of Biochemical and Pharmaceutical Research., 1(4) (2013).

Kunamneni Adinarayana, Bezawada Jyothi and Poluri Ellaiah. Production of Alkaline Protease With Immobilized Cells of Bacillus subtilis PE-11 in Various Matrices by Entrapment Technique. AAPS PharmSciTech 6 (3) (2005).

Lingappa Kattimani, Syeda amena, Vishalakshi N and Prabhakar M. immobilization of Streptomyces gubergensis in polyurethane foam: A promising technique for L-Asperginase Production. Iranian Journal of biotechnology. 7 (4) (2009).

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