Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > ILNS Volume 30 > Studies on Haemolytic Properties of Puffer Fishes...
< Back to Volume

Studies on Haemolytic Properties of Puffer Fishes from South East Coast of India

Full Text PDF


Marine fishes play an important role for health care. The objectives of the present study was to evaluate the haemolytic activity of crude extracts of six puffer fishes Cyclichthys orbicularis, Diodon holocanthus, Canthigaster solandri, Arthron hispidus, A. inermis and Lagocephalua inermis collected from Parangipettai, Tamil Nadu, South East Coast of India. The haemolytic activity was tested against red blood cells (RBCs) of chicken, goat and human blood. The haemolytic activity was high in chicken blood (128HU) against of Cyclichthys orbicularis and A. inermis and minimum (16HU) in Lagocephalua inermis. In goat erythrocytes the highest haemolytic activity (256HU) against Arthron hispidus and minimum (16HU) against A. inermis and Lagocephalua inermis were observed. In human erythrocytes the maximum haemolytic activity of 32HU against Cyclichthys orbicularis, Arthron hispidus were recorded. In blood agar plate assay the highest zone of inhibition of 6.4±0.9 and 6.3±0.1 mm were observed in A. inermis against chicken erythrocytes and Arthron hispidus against goat erythrocytes respectively. The results strongly suggest that, the Marine puffer fish extracts showed good cytolytic properties against blood RBCs.


International Letters of Natural Sciences (Volume 30)
M. M. Raj et al., "Studies on Haemolytic Properties of Puffer Fishes from South East Coast of India", International Letters of Natural Sciences, Vol. 30, pp. 11-18, 2015
Online since:
December 2014

Al-Hassan J.M., M. Thomsson, R.S. Criddle, Mar. Biol. 70 (1982) 27-33.

Bragadeeswaran S., Priyadharshini S., Prabhu K, Sophia R.S., Asian Pac. J. Trop. Med. 4(5) (2011) 412-420.

Brgadeeswaran,S., D. Theras, K. Prabhu and K. Kathiresan, J. Venom Anim. Toxins Incl. Trop. Dis. 16 (2010) 421-431.

Faulkner D. J., Nat. Prod. Rep. 18 (2001) 1-49.

Halstead B. W. (1978). Poisonous and venous marine animals of the world. Review, (eds), Princeton, NJ: Darwin Press.

Karthikayalu S., Rama V., Kirubagaran R., Venkatesan R., J. Ven. Anim. Toxin incl. Trop. Dis. 16(2) (2010) 223-240.

Lemes-Marques E. G., Yano T., FEMS Microbiol. Lett. 239 (2004) 63-70.

Prasad K. P., Venkateshvaran K. (1997). Microhaemolytic Assay. In: International Training Manual on Advance Techniques in Marine Biotoxinology, Venkateshvaran, K. and K.P. Prasad (Eds. ). Central Institute of Fisheries Education, India. P 41.

Shier W. T. (1988). Cytotoxic Effect of Marine Toxins and venoms. in: Handbook of Natural Toxins. vol. 3. Marine Toxins and Venoms, Anthony T. Tu. (ed. ), Marcel and Dekker Inc, Newyork, U.S.A. pp.477-486.

Simonsen S. M., J. Biol. Chem. 283(15) (2008) 9805-9813.

Venkatachalam Uthayakumar, Venkatachalam Ramasubramanian, Dhanabalan Senthilkumar, Venkatesan Brindha Priyadarisini, Ramasamy Harikrishnan, Asian Pacific Journal of Tropical Biomedicine (2012) S863-S869.

Wright A.C., R.T. Hill, J.A. Johnson, M.C. Roghman, R.R. Colwell, J.G. Morris, Appl. Environ. Microbial. 62 (1996) 717-724.

Wu Z., Y. Young, Xie G. Xia, J. Hu, S. Wang, R. Zhang, Sea of China. Toxi. 46(4) (2005) 471-476.

Yu C., P.H. Yu, P. Chan, Q. Yan, P. Wong, Toxi. 44(6) (2004) 641-647. ( Received 08 November 2014; accepted 16 November 2014 ).

Show More Hide
Cited By:
This article has no citations.