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Effect of Chromium on Growth, Biochemicals and Nutrient Accumulation of Paddy (Oryza sativa L.)

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Chromium is a heavy metal; this element is considered as an environmental hazard. Toxicity effects of chromium on growth and development of plants including inhibition of germination process decrease of growth and biomass of plant. The aim of this research is to study accumulation of Chromium along with nutrients and its effect on the growth of Paddy plant (Oryza sativa L). Thus, paddy seedlings grown in petriplates lined with filter paper undergoing, different treatments of Cr (0, 2.5, 5, 10, 25, 50, 75, 100 and 200 mg/L). After one week seedlings were removed and morpho physiological parameters like root length, shoot length and dry weight of plants and Biochemicals ,accumulation of nutrients along with Cr in roots and shoots were determined. The results indicated that the concentrations more than 100 mg/L chromium cause the reduction of morphophysiology parameters in the treatment plants rather than control plant and Cr addition in the cultures caused enhancement of chromium content in roots and shoots of plant seedlings. Similarly the biochemicals and nutrient accumulation also affected by increasing concentrations of chromium. It was also noted that accumulation of chromium in the roots was much higher than the shoots of the seedlings under treatment.


International Letters of Natural Sciences (Volume 23)
M. Nagarajan and K. S. Ganesh, "Effect of Chromium on Growth, Biochemicals and Nutrient Accumulation of Paddy (Oryza sativa L.)", International Letters of Natural Sciences, Vol. 23, pp. 63-71, 2014
Online since:
August 2014

Abbassi S.S., Abbassi N., Soni R. (1998). Heavy metals in the environment, Mittal Publication, New Delhi, India.

Ali NA, Ater M.; Sunahara, Gl. and Robidoux, P.Y. (2004).

Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts polyphenol oxidase in Beta vulgaris. Plant Physiol., 24: 1-15.

Assche F. Van, H. Clijsters, Plant Cell Environ. 13 (1990) 195-206.

Bitell, J.E., D.E. Koeppe and R.J. Miller, 1974. Sorption of heavy metal cations by corn mitochondria and the effects on electron and energy transfer reactions. Physiol. Plantarum, 30: 226-230.

Black, C.A., 1965. In: Methods of Soil Analysis Part 2. Chemical and Microbiological Properties, American Society of Agronomy, Inc., Madison, Wisconsin, p.242.

Cervantes, C.; Campos-Garcia, J.; Debars, S.; Gutierrez-Corona, F.; Loza-Tavera, H.; Carlos-Tarres-Guzman, M. and Moreno-Sanchez, R. (2001). Interaction of chromium with Microgenesis and plants. FEMS Microbiol. Rev., 25: 335-347.

Chatterjee J., Chatterjee, C., Environ. Pollut. 109 (2000) 69-74.

Crooke W.M., R.H.E. Inkson, Plant Soil 6 (1995) 1-15.

Dua A., S.K. Sawhney, Environ. Exp. Bot. 31 (1991) 133-139.

Dube, B.K.; Tewari, K.; Chatterjee, J. and Chaterejee, C. (2003). Excess chromium alters uptake and translocation of certain nutrients in citrullus. Chemosphere 53: 1147-1153.

Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-356.

Jackson, M.L., 1958. Soil chemical analysis. Prentice Hall of India Private Limited, New Delhi, pp.22-23.

Kirk, J.T.O. and R.L. Allen, 1965. Dependence of chloroplast pigments synthesis on protein synthetic effects of acitilione. Biochem. Biophys. Res. Commun., 27: 523-530.

Krishnamurthy S., Wilkens M.M., Northeasteren geology 16 (1994) 14-17.

Lakshmi S., P. Sundaramoorthy, J. Ecobiol. 15 (2003) 7-11.

Lalitha, K., N. Balasubramanian and S. Kalavathy, 1999. Studies of impact of chromium on Vigna unguiculata (L. ) Walp. var. Long. J. Swamy Bot. Cl., 16: 17-20.

Lanoreaux R.J.W.R., S. Chaney, Plant Physiol. 43 (1978) 231-236.

Lowry, O.H., N.J. Rosenbrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with folin-phenol reagent. J. Biol. Chem., 193: 265-275.

Mayz D.M.J., P.M. Cartwright, Plant Soil 80 (1984) 423-430.

Moore, S. and W.H. Stein, 1948. Photometric method for use in the chromatography of amino acids. J. Biol. Chem., 176-388.

Nelson, N., 1944. A photometric adaptation of the Somogyis method for the determination of reducing sugar. Anal. Chem., 3: 426-428.

Pillay A. E., et al., Environ. Intl. 1048 (2003) 1-5.

Piper, C., 1966. Soil and plant analysis. Asian Hans Publishers, Bombay, pp.11-36.

Rai U. N., Tripathi R. D., Kumar N., Chromosphere 25 (1992) 721-732.

Rout, G.R., S. Samantary and P. Das, J. Plant Nutr. 20 (1997) 473-483.

Samantary, S. and B. Deo, 2004. Studies on chromium toxicity in mung bean (Vigna radiata L. ). Adv. Plant Sci., 17: 189-194.

Sankar Ganesh K., AL.A. Chidambaram, P. Sundaramoorthy, L. Baskaran M. Selvaraj (a), Indian J. Environ. Ecoplan. 12 (2006) 291-296.

Sankar Ganesh K., L. Baskaran, S. Rajasekaran, K. Sumathi, AL.A. Chidambaram. P. Sundaramoorthy, Colloid. Surface. B 63 (2008) 159-163.

Sankar Ganesh, K., P. Sundaramoorthy and AL.A. Chidambaram, 2006b. Chromium toxicity effect on blackgram, soybean and paddy. Poll. Res., 25: 257-261.

Shanker A.K., C. Cervantes, H.L. Tavera, S. Avudainanyagam, Environ. Int. 31 (2005) 739-753.

Shanker A.K., M. Djanaguiraman, R. Sudhagar, C.N. Chandrashekar, G. Pathmanabhan (a), Plant Sci. 166 (2004) 1035-1043.

Sharma, D. C.; Chatterjee, C. and Sharma, C. P. (1995). Chromium accumulation by barley seedlings (Hordeum vulgare L, ). Journal of experimental botany 25: 241-251.

Sharma D.C., Pant R. C., Journal of environmental science and health, Part A 29 (1994) 941-948.

Sidharthan, M. and A.S. Lakshmanachary, 1996. Efficacy of chromium on germination, growth and biochemical studies on Glycine max var. CO 1. In: Jha, P.K., G.P.S. Ghirmire, S.B. Kamacharya, S.R. Baral and P. Lacoul (eds. ), Environment and Biodiversity, Ecological Society, Katmandu, Nepal, pp.326-328.

Subramani, A., P. Sundaramoorthy, S. Saravanan, M. Selvaraj and A.S. Lakshmanachary, 1999. Screening of groundnut cultivars for chromium sensitivity. Ecoprint, 6: 61-65.

Sundaramoorthy, P., K. Sankar Ganesh, L. Baskaran, K. Sumathi, S. Rajasekaran, 2006b. Germination behaviour of some agricultural crops under chromium treatment. Bull. Biol. Sci., 4: 99-101.

Sundaramoorthy, P., K. Sankar Ganesh, S. Rajasekaran, L. Baskaran and K. Sumathi, 2006a. Studies on the effect of chromium on germination and growth of soybean (Glycine max) cultivars. Bull. Agr. Sci., 4: 91-94.

Williams, C.H. and V. Twine, 1960. In: Peach, K. and M.V. Tracey (eds. ), Modern Methods of Plant Analysis, Vol. 5, Springer Verlag, Berlin, pp.3-5.

Yoshida, S., D. Fordo, J. Cork and K. Gomez, 1972. Laboratory manual for physiological studies of rice, 3rd edn., The International Rice Research Institute, Philippines, pp.11-23.

Zayed A. M., Terry N., Plant and soil 249 (2003) 139-156.

Zayed A., Lytle C.M., Qian J.H., Terry N., Planta 206 (1998) 293-299. ( Received 10 August 2014; accepted 19 August 2014 ).

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