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Effects of NaCl, CaCl2 and their Combination of Salt on Seed Germination and Seedling Growth of Lycopersicum esculentum L.

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To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling growth stages of Lycopersicum esculentum L., were studied under pot experiments 2008. Results indicated that significant increases were recorded in percentage of germination, seedling fresh and dry weights, seedling length, water content, catalase activity and photosynthetic pigments (chlorophyll a, b and total chlorophylls as well as carotenoids) under the low level concentration (20 mM) of NaCl or CaCl and their combination (1:1). On other hand increasing salt concentration in nutrient solution caused significant decrease in all of these parameters. The great reduction occurred under high salinity level of NaCl (50 mM). Meanwhile, peroxidase activity increased significantly with increasing salinity levels from 20 mM to 50 mM of both applied salinity types. Besides, peroxidase activity under NaCl salinity showed a marked increase followed by NaCl + CaCl2 (1:1) and CaCl2 at 50 mM.


International Letters of Natural Sciences (Volume 22)
S. Sivasankaramoorthy "Effects of NaCl, CaCl2 and their Combination of Salt on Seed Germination and Seedling Growth of Lycopersicum esculentum L.", International Letters of Natural Sciences, Vol. 22, pp. 1-15, 2014
Online since:
Aug 2014

Lycoskoufis, I.H., Savas, D. and Mavrogianopouls, G. 2005. Growth gas exchange and nutrient status in pepper (Capsium annuum L. ) grown in recirculating nutrient solution as affected by salinity imposed to half of the root system. Scientia Hort., 106: 147-161.

Ashraf, M. and Foolad. M.R. 2005. Per-sowing seed treatment a shotgun approach to improve germination, plant growth, and crop yield under saline and non- saline conditions. Advances in Agronomy, 88: 223-371. 0 0. 05 0. 1 0. 15 0. 2 0. 25 0. 3 0. 35 Carotenoids (mg/g FW) Salinity.

Khan, A., M.S.A. Ahma, H.U. Athar and M. Ashraf, 2006. Interactive effect of foliarly applied ascorbic acid and salt stress on wheat (Triticum aestivum L. ) at the seedling stage. Pak. J. Bot., 38: 1407-1414.

Bassuony, F.M. Hassanein, R.A. Baraka, D.M. and Khalil, R.R. 2008. Physiological effects of nicotinamide and ascorbic acid on Zea mays plant grown under salinity stress ii- changes in nitrogen constituents, protein profiles, protease enzyme and certain inorganic cations. Aust. J. basic and App. Sci., 2(3): 350-359.

Almansouri, M. Kinet, M. and Lutts, S. 2001. Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Dest). Plant and Soil, 231: 243-254.

Delachiave, M.E.A. and De Pinho, S.Z. 2003. Scarification, temperature and light in germination of Senna occidentalis seed (Caesalpinaceae). Seed Sci. and Techology, 31(2): 225-230.

Stepien, P. and G. Klobus, 2006. Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress. Biol. Plant, 50: 610-616.

Hasegawa, P.M., Bressan, R.A., Zhu, J. K, and Bohnert, H.J. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol., 51: 463-499.

Winston, G.W. 1990. Physio-chemical basis for free radical formation in cells: production and defenses, pp.57-86.

Prochazkova, D. and Wilhelmova, N. 2007. Leaf senescence and activities of the antioxidant enzymes. Boil. Plant, 51: 401-406.

Ashraf, M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances, 27: 84-93.

Cooper, A., 1979. The ABC of NFT. Growers Books, London, P. 59.

Song, J.Q. and Fujiyama, H. 1998. Importance of Na content and water status for growth in Na-salinized rice and tomato plants. Soil Sci. Plant. Nutr, 44: 197-208.

Maxwell, D.P. and Bateman, D.F. 1967. Changes in the activities of some oxidases in extracts of Rhizoctonia infected bean hypocotyls in relation to lesion maturation. Phytopathology, 57: 132.

Aebi, H. 1984. Catalase in vitro. Metheds Enzymol., 105: 121-126.

Robinson, S.P., Downton, W.J.S. and Milhouse, J.A. 1983. Photosynthesis and ion cintent of leaves and isolate chloroplasts of salt-stressed spinach. Plant Physiol., 73: 238-242.

Mackiny, G. 1941. Absorption of light by chlorophyll solution. J. Biol. Chem., 140: 315-322.

Khajeh-Hosseini M, Powell AA, Bimghan IJ (2003). The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci. Technol. 31: 715-725.

Gomes Filho, E. and Scodek, L. 2002. Effect of salinity on ribonuclease activity of Vigna unguiculata cotyledons during germination. J. plant Physilogy, 132: 307-311.

Elouaer MA, Hannachi C (2012). Seed priming to improve germination and seedling growth of sanflower (Carthamus tinctorius) under salt stress. Eurasian J. Biosci. 6: 76-84.

Almodares, A., Hadi, M.R. and Dosti, B 2007. Effects of salt and stress on germination percentage and seedling growth in sweet sorghum cultivars. J. Biological scinces., 7(8): 1492-1495.

Taamalli, W., Abz, L., Youssef, N.B., Miled, D.D.B. and Zarrouk, M. 2004. Lipid breakdown in sunflower (Helianthus annuus L. ) seeds during post germinative growth under salt-stress. Rivista Italian delle Sostanze Grasse, 81: 90-97.

Ayaz, F.A. Kadioglu, A. and Turgut, R. 2000. Water stress effects on the content of low molecular weight carbohydrates and phenolic acids in Ctenenthe setosa (Rose). Eichler, Can. J. Plant Sci., 80: 373-378.

Greenway, H. 1963. Plant responses to saline substrates. Effect of nutrient concentration on the growth and ion uptake of Hordeum Vulgare during NaCl stress. Aust. J. Biol. Sci., 16: 616-619.

Hatung, W. 2004. Plant response to stress. Abscisic acid fluxes. Marcel Dekker. Inc., New York.

Hawker, J. S,. and R.R. Walker, 1978. Effects of sodium chloride on expansion rates and invertase activity of leaves. Aust. J. Plant Physiol., 5: 73-80.

Schwarz, M. 1985. The use of saline water in hydroponics. Soilless Cult., 1: 25-34.

Yeo, A.R. 1983. Salinity resistance: physiologies and prices. Physiol. Plant., 58: 214-222.

Younis, M.E., El-Shahaby, O.A., Nemat Ally, M.M. and El-Bastawisy Zeinab, M. 2003. Kinetin alleviates the influence of water logging and salinity in Vigna sinensis and Zea mays. Agronome, 23: 277-285.

Aboshama, H.M.S. and Hegazy, A.E. 2009. In vitro screening and production of salt tolerant Critrus volkamariana plant. J. Agric. Sci. Mans. Univ., 34 (10): 10115-10133.

Tripathi, S.B., Gurumurthi, K., Panigahi, A.K. and Shaw, B.P. 2007. Salinity induced changes in proline and betaine contents and synthesis in two aquatic macrophytes differing in salt tolerance. Biol. Plant, 51: 110-115.

Hajibagheri, M.A., You, A.R., Flowers, T.J. and Collins, J.C. 1989. Salinity resistance in Zea mays fluxes of potassium, sodium and chloride, cytoplasmic concentrations and microsomal membrane lipids. Plant cell and Env., 12: 753-757.

Meloni, D.A., Gulotta, M.R. and Martinez, C.A. 2008. Salinity tolerance in Schinopsis quebracho Colorado. Seed germination, growth, ion relations and metabolic responses. J. Arid Env., 72: 1785-1792.

Munns, R., R.A. James and A. Lauchli, 2006. Approaches to increasing the salt tolerance of wheat and other cereals. J. Exp. Bot., 57: 1025-1043.

Gechev, T.S., Breusegem, F.V., Stone, J.M., Denev., I. and Laloi, C. 2006. Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bio. Essays, 28: 1091-1101.

Jaleel, C.A., P. Manivannan, G.M.A. Lakshmanan, R. Sridharan and R. Panneerselvam, 2007. NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus. C. R. Biologies, 330: 806-813.

Mittova, V., M. Tal, M. Volokita and M. Guy, 2003. Upregulation of the leaf mitochondriral and peroxisomal antioxidative systems in response to salt induced oxidative stress in the wild salt tolerant tomato species Lycopersicon pennelli. Plant Cell Env., 26: 845-858.

Ali, A.A. and Alqurainy, F. 2006. Activities of antioxidants in plants under environment stress. In: Motohashi, N. The lute in-prevention and treatment for diseases. India: Transworld research network, pp.187-256.

Jiang, M.Y., 1999. Generation of OH and oxidation injury of plants under the condition of water stress. Acta Bot. Sinica., 41(3): 229-234.

Turhan, E., L. Karni, H. Aktas, G. Deventurero, D.C. Chang, A. Bar-Tal and B. Aloni, 2006. Apoplastic anti-oxidants in pepper (Capsicum annuum L. ) fruit and their relationship to blossom-end rot. J. Hort. Sci. Biot, 81(4): 661-667.

Li, D., C. Li, H. Sun, W. Wang, L. Liu and Y. Zhang, 2010. Effects of drought on soluble protein content and protective enzyme system in cotton leaves. Front. Agric. China, 4: 56-62.

Wang, W.B., Y.H. Kim, H.S. Lee, K.Y. Kim, X.P. Deng and S.S. Kwak, 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol. & Bioch., 47: 570-577.

Noreen, Z. and M. Ashraf, 2009. Assessment of variation in antioxidant defense system in salt treated pea (Pisum sativum) cultivars and it putative use as salinity tolerance markers. J. Plant Physiol., 166: 1764-1774.

Hassanein, R.A., A.A. Hassanein, A.A. Haider and H.A. Hashem, 2009. Improving salt tolerance of Zea mays L. plants by presoaking their grains in glycine betaine. Aus. J. Basic. Appl. Sci., 3: 928-942.

Gadalla, S.F. 2009. The roles of ascorbic acid α-tocopherol in minimize of salt-induced whert leaf senescence. J. Agric. Sci. Mans. Univ., 34 (11): 10645-10661.

Lichtenthaler, HK. Langsdorf, G, Lenk S, and Bushman, C (2005). Chlorophyll fluorescence imaging of photosynthetic activity with the lamp fluorescence imaging system, phtosynthetica 43: 355-369.

Montesano, F and Van lersel MW (2007). Calcium can prevent toxic effects of Na+ on tomato leaf photosynthesis but does not restore growth. J Amer Soc Hort Sci 132: 310-318.

Afzal I, Butt A, Rehman, HU, Basra S and Afzal, A. 2012. Alleviation of salt stress in fine aromatic rice by seed priming. Australian J Crop Sci 6: 1401-1407.

Yeo, AR, lee KS. Lizard P. Boursier PJ, Flowers TJ. (1991). Short and long term effects of salinity on leaf growth in rice (Oryza sativa). J Exp Bot 42: 881-889.

Parida, A.K., Das, A.B. and Mittra, B. 2004. Effects of salt on growth, ion accumulation, photosynthesis and leaf anatomy of the mangrove, Bruguiera parviflora. Trees-Struct. Funct., 18: 167-174.

Helaly, M.N., A.M. Salama and A.A. Arafa, 1984. Effects of salinity on growth mineral constituents, water fractions and endogenous growth substances in Horse bean plants. J. Agric. Sci. Mans. Univ., 9(2): 251-264.

Mishra, S.N. and I. Sharma, 1994. Putrescine as growth inducer and nitrogen source for mustard seedling grown under salinity. Int. J. Exp. Biol., 32: 916-918.

Pell, E.J. and M.S. Dann, 1991. Multiple stress-induced foliar senescence and implication for whole plant longevity. In. Response of plants to multiple stresses. (Eds. A.H. Mooney, V.E. Vinner, E.J. Pell and E. Chu), Academic Press Inc., pp.189-204.

Hashem, H.A., 2000. Molecular physiological studies on heat shock protein expression in a stressed plant. M. Sc. Thesis, Fac. Sci. Ain Shams Univ., Cairo, Egypt, (Thesis unpublished). ( Received 22 July 2014; accepted 02 August 2014 ).

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