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Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (Cajanus cajan (L.) Millsp)

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In the present investigation the seeds of Pigeon pea (Cajanus cajan (L.) Millsp) were treated with different doses of gamma radiation (05KR, 10KR, 15KR, 20KR, 25KR, 30KR, 35KR, 40KR, 45KR, and 50KR) and concentration of Ethyl Methane Sulphonate (05mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM, and 50mM) for studying seed germination, seedling height, (shoot and root), seedling injury, seedling vigour index, and seedling survival of plants at 30th day. The seed germination percentage was decreased with increased in the concentration/doses when compared to control. The LD50 (Lethal dose) value was determined based upon the seed germination percentage. The 50 percentage of seed germination and reduction was observed in 20KR of gamma rays and 25mM of EMS and it is considered as LD50 value for both the treatments. The decrease in seed germination was more prominent with gamma rays than that of EMS treatments. The seedling parameters of gamma rays and EMS treated seedlings were progressively decreased with increase dose/concentration in all mutagenic treatments when compared to control. The maximum seedling parameters were observed in 05KR of gamma rays and 05mM of EMS. Minimum seedling parameters were observed in 50mM of EMS and 50KR of gamma rays respectively.


International Letters of Natural Sciences (Volume 21)
M. Ariraman et al., "Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (Cajanus cajan (L.) Millsp)", International Letters of Natural Sciences, Vol. 21, pp. 41-49, 2014
Online since:
Jul 2014

Akgun I., Tosum M. (2004). Agricultural and cytological characteristics of M1 perennial rye (Secale montanum Guss. ) as effected by the application of different doses of gamma rays. Pakistan Journal of Biological Science, 7(5); 827-833.

Amernath S. and A.B. Prasad, (1998), Induced variability in homozygous and heterozygous genotypes of tobacco. Indian Journal of Genetics, 58(1), 69-77.

Amernath S. and A.B. Prasad, (1998), Induced variability in homozygous and heterozygous genotypes of tobacco. Indian Journal of Genetics 58(1), 69-77.

Ashraf, M., Cheema, A. A., M. Rashid, Zia-ul-Qamar. (2003). Effect of gamma rays on M1 generation in basmati rice. Pakistan Journal of Botany, 35(5), 791-795.

Auti S. G. Mutational Studies in mung (Vigna radiata (L. ) Wilczek). Ph.D. Thesis. (2005); University of Pune, Pune (MS), India.

Barshile JD. Studies on effect of mutagenesis employing EMS, SA and GR in Chickpea (Cicer arietinum L. ). Ph.D. Thesis. (2006); University of Pune, Pune (MS), India.

Bharathi T., Gnanamurthy, S., D. Dhanavel, S. Murugan, M. Ariraman, (2013).

Dalvi V.V. (1990). Gamma rays induced mutagenesis in horsegram (Macrotyloma uniflorum (Lam. ) Database. Mut. Breed. Rev., 12, 1-12.

Datir S.S., Dhumal K.N., Pandey R.N., Gamma radiation and EMS induced variation in seed germination and plant survival in horsegram (Macrotyloma uniflorum (Lam. )Verdc). J. Arid Legumes. 4(1) (2007) 15-17.

Dhanavel D, Pavadai P, Mullainathan L, Mohana D, Raju G, Girija M and Thilagavathi C. (2008). Effectiveness and efficiency of chemical mutagens in cowpea (Vigna unguiculata (L. ) Walp). African J. of Biotechnology 7(22): 4116-4117.

Din R., M.M. Khan, M. Qasim, S. Jehan, M.I. Khan (2003). Induced Mutability Studies in three Wheat (Triticum aestivum L. ) varieties for Some morphological and agronomic characteristics. Asian Jour. of Plant Sciences, 2(17): 1179-1182.

Gaul H. (1977).

Hajara N.G. (1979). Induced of mutations by chemical mutagens in tall indica rice. Indian Agric., 23: 67-72.

Jayakumar S., R. Selvaraj (2003). Mutagenic effectiveness and efficiency of gamma rays and ethyl methane sulphonate in sunflower. Madras Agriculture Journal, 90(7-9), 574-576.

Kavithamni D., Kalamani A., Vannirajan C., Uma D. (2008). Mutagenic effectiveness and efficiency of gamma rays and EMS in Soybean (Glycine max (L. ) Merrill). Agric. J. 95(7-12), 448-451.

Khan M.R., A.S. Qureshi, A.H. Syed, M. Ibrahim (2005). Genetic variability induced by gamma irradiation and its modulation with gibberellic acid in M2 generation of Chickpea(Cicer arietinum L. ). Pakistan Journal of Botany, 37(2), 285-292.

Kim Y., K.S. Schumaker, J.K. Zhu (2006). EMS mutagenesis of Arabidopsis. Meth. Mol. Biol. 323: 101103.

Kovacs E., Keresztes A. (2002). Effect of gamma and UV-B/C radiation on plant cells. Micron, 33(2), 199-210.

Kumar A., M.N. Mishra (2004). Effect of gamma-rays, EMS and NMU on germination, seedling vigour, pollen viability and plant survival in M1and M2 generation of Okra (Abelmoschus esculentus (L. ) Moench). Advances in Plant Science, 17(1), 295-297.

Lee Y. I., I.S. Lee, Y.P. Lim (2002). Variation in sweed potato regenerates from gamma-rays irradiated embryogenic callus. J Plant Biotech 4, 163-170.

Maluszynski, K.N., L.V. Zanten, B.S. Ahloowalia (2000). Officially released mutant varieties. The FAO/IAEA.

Menda N., Y. Semel, D. Peled, Y. Eshed, D. Zamir (2004). In silico screening of a saturated mutation library of tomato. Plant J. 38: 861872.

Mohamad O., Herman, S., Nazir, B.M., Shamsudin, S., Takim, M. (2005).

Okagaki R.J., M.G. Neffer, S.R. Wessler (1991). A deletion common to two independently derived waxy mutations of maize. Genetics, 127: 425-431.

Potdukhe N.R., Narkhede M.N. (2002). Induced mutation in pigeonpea (Cajanus cajan (L. ) Millsp. ). J. Nuclear Agric. Biol. 31 (1): 41-46.

Reddy V.R.K. and P.K. Gupta, (1989), Biological effects of gamma rays and EMS in hexaploid Triticale. Acta Botanica, 17, 76-86.

Sayed H.I. (1975). Effects of gamma rays and some chemical mutagens on induction of Telotrisomocs in Hordeum. Can J. Genetics and Cytology. XV: 815-825.

Siddiqui B.A., S. Khan (1999). Breeding in Crop Plants: Mutations and in Vitro Mutation Breeding. 1st ed. Kalyani Publishers, Ludhiana.

Singh M., Singh V.P. (2001). Genetic analysis of certain mutant lines of urdbean for yield and quality traits in M4 generation. Ind. J. Pulses Res. 14(1): 60-62.

Sree Ramulu (1970). Sensitivity and induction of mutations in Sorghum. Mutation research. 10: 197-205.

Studies on the mutagenic effect of EMS on seed germination and seedling characters of Sesame (Sesamum indicum L. ) Var. T MV3. International Journal of Research in Biological Sciences 3(1): 68-70.

Sunil M. Sangle, Swapnil E. Mahamune, Sopon N. Kharat, V. S. Kothekar. (2011). Effect of mutagenisis on germination and pollen steritity inpigeonpea. Bioscience discovery l02(1).

Thapa C.B. (2004). Effect of acute exposure of gamma rays on seed germination and seedling growth of Pinus kesiya Gord and P. wallichiana A.B. Jacks. Our Nature, 2, 13-17.

Toker C., B. Uzen, H. Canci, F.O. Ceylan (2005). Effects of gamma irradiation on the shoot length of Cicer seeds. Radiation Physics and Chemistry, 73, pp.365-367.

Wongpiyasatid A., Chotechuen S., Hormchan P. (2000). Induced mutations in mungbean breeding Regional yield trail of mungbean mutant lines. Kasetsart J. (Nat. Sci. ). 34: 443-449.

Zaka R., C. Chenal, M.T. Misset (2004). Effect of low doses of short-term gamma radiation on growth and development through two generations of Pisum sativum. Science of the Total Environment, 320, 121-129.

M. E. Emefiene, A. B. Salaudeen, A. Y. Yaroson, International Letters of Natural Sciences 1 (2013) 6-16.

M. E. Emefiene, V. I. Joshua, C. Nwadike, A. Y. Yaroson, N. D. E. Zwalnan, International Letters of Natural Sciences 13(2) (2014) 73-88. ( Received 22 July 2014; accepted 29 July 2014 ).

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