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Media Optimization for Depolymerization of Alginate by Pseudomonas aeruginosa AG LSL-11

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Abstract:

An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.

Info:

Periodical:
International Letters of Natural Sciences (Volume 19)
Pages:
30-39
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.19.30
Citation:
B. A. Koti et al., "Media Optimization for Depolymerization of Alginate by Pseudomonas aeruginosa AG LSL-11", International Letters of Natural Sciences, Vol. 19, pp. 30-39, 2014
Online since:
Jul 2014
Authors:
Basawaraj A. Koti, S. Manohar, J. Lalitha
Keywords:
Alginase, Bacterium, Pseudomonas aeruginosa
Export:
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Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

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