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International Letters of Natural Sciences
Volume 17

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Optimization of Agrase Production by Alkaline Pseudomonas aeruginosa ZSL-2 Using Taguchi Experimental Design

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Abstract:

The culture conditions for the production of extracellular agarase by Pseudomonas aeruginosa ZSL-2 were optimized using One-Factor-At-A-Time combined with orthogonal array design. One-Factor-At-A-Time method investigates the effect of time, temperature, NaCl, carbon sources, nitrogen sources and pH on agarase production. The optimized culture conditions obtained from the statistical analysis were temperature of 30 °C, pH 8.5, NH4NO3 2 g L-1 and agar 3 g L-1. The L9 orthogonal array design was used to select the fermentation parameters influencing the yield of agarase. The order of the factors affecting the fermentation process was found to be NH4NO3 > pH > agar > temperature, with temperature playing a significant role on the agarase production (p < 0.10). The higher yields than those in basal media culture were obtained in the final optimized medium with activity of 0.439 ± 0.013 U ml-1. Extracellular agarase hydrolysed agar into a range of oligosaccharides which were analysed by LC-ESI-MS spectrometry as anhydrogalactose, galactose, agarobiose, agarotetrose and agarohexaose.

Info:

Periodical:
International Letters of Natural Sciences (Volume 17)
Pages:
180-193
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.17.180
Citation:
M. Ziayoddin et al., "Optimization of Agrase Production by Alkaline Pseudomonas aeruginosa ZSL-2 Using Taguchi Experimental Design", International Letters of Natural Sciences, Vol. 17, pp. 180-193, 2014
Online since:
Jun 2014
Authors:
M. Ziayoddin, Junna Lalitha, Manohar Shinde
Keywords:
Agar, Agarase, L9 Orthogonal Array Design, LC-ESI-MS, Pseudomonas aeruginosa ZSL-2
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Distribution:
Open Access

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This work is licensed under a
Creative Commons Attribution 4.0 International License

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