Paper Titles in Periodical
International Letters of Natural Sciences
ILNS Volume 17
Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILNS > ILNS Volume 17 > Optimization of Agrase Production by Alkaline...
Optimization of Agrase Production by Alkaline <i>Pseudomonas aeruginosa</i> ZSL-2 Using Taguchi Experimental Design
< Back to Volume

Optimization of Agrase Production by Alkaline Pseudomonas aeruginosa ZSL-2 Using Taguchi Experimental Design

Full Text PDF

Abstract:

The culture conditions for the production of extracellular agarase by Pseudomonas aeruginosa ZSL-2 were optimized using One-Factor-At-A-Time combined with orthogonal array design. One-Factor-At-A-Time method investigates the effect of time, temperature, NaCl, carbon sources, nitrogen sources and pH on agarase production. The optimized culture conditions obtained from the statistical analysis were temperature of 30 °C, pH 8.5, NH4NO3 2 g L-1 and agar 3 g L-1. The L9 orthogonal array design was used to select the fermentation parameters influencing the yield of agarase. The order of the factors affecting the fermentation process was found to be NH4NO3 > pH > agar > temperature, with temperature playing a significant role on the agarase production (p < 0.10). The higher yields than those in basal media culture were obtained in the final optimized medium with activity of 0.439 ± 0.013 U ml-1. Extracellular agarase hydrolysed agar into a range of oligosaccharides which were analysed by LC-ESI-MS spectrometry as anhydrogalactose, galactose, agarobiose, agarotetrose and agarohexaose.

Info:

Periodical:
International Letters of Natural Sciences (Volume 17)
Pages:
180-193
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.17.180
Citation:
M. Ziayoddin et al., "Optimization of Agrase Production by Alkaline Pseudomonas aeruginosa ZSL-2 Using Taguchi Experimental Design", International Letters of Natural Sciences, Vol. 17, pp. 180-193, 2014
Online since:
June 2014
Authors:
M. Ziayoddin, Junna Lalitha, Manohar Shinde
Keywords:
Agar, Agarase, L9 Orthogonal Array Design, LC-ESI-MS, Pseudomonas aeruginosa ZSL-2
Export:
RIS, BibTeX, Text
Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

Kobayashi R., M. Takisada, T. Suzuki, K. Kirimura, S. Usami Biosci. Biotechnol. Biochem. 61 (1997) 162-163.

Osumi Y., M. Kawai, H. Amano, H. Noda Nippon Suisan Gakkaishi 64 (1998) 98-104.

Hu B., Q. Gong, Y. Wang, Y. Ma, J. Li, W. Yu Anaerobe. 12 (2006) 260-266.

Lavilla-Pitogo C. R., Aquaculture 102 (1992) 1-7.

Schroeder D. C., M. A. Jaffer, V. E. Coyne, Microbiology 149 (2003) 2919-2929.

Agbo J. A. C., M. O. Moss, J. Can. Microbiol. 115 (1979) 355-368.

Stanier R. Y., J. Bacteriol. 44 (1942) 555-570.

Suzuki H., Y. Sawai, T. Suzuki, K. Kawai, J. Biosci. Bioeng. 95 (2003) 328-334.

Hosoda A., M. Sakai, S. Kanazawa, Biosci. Biotechnol. Biochem. 67 (2003) 1048-1055.

Lakshmikanth M., S. Manohar, J. Patnakar, P. Vaishampayan, Y. Shouche, J. Lalitha World J. Microbiol. Biotechnol. 22 (2006a) 531-537.

Changkyou, J., K. Hyuckjin, P. Chulhwan, L. Jinwon, Biotechnol. Bioproc. Eng. 17(5) (2012) 937-945.

Van der Meulen H. J., W. Harder, Antonie. Van. Leeuwenhoek. 41 (1975) 431-447.

Hu Z., B. K. Lin, Y. Xu, M. Q. Zhong, G. M. Liu, J. Appl. Microbiol. 106 (2009) 181190.

Fu X. T., H. Lin, S. M. Kim, Proc. Biochem. 44 (2009) 1158-1163.

Choi H. J., J. B. Hong, J. J. Park, W. J. Chi, M. C. Kim, Y. K. Chang, S. K. Hong, Biotechnol. Bioproc. Eng. 16 (2011) 81-88.

Khambhaty Y., K. Mody, B. Jha, Biotechnol. Bioproc. Eng. 13 (2008) 584-591.

Ziayoddin M., S. Manohar, J. Lalitha, The Bioscan. 5 (2010) 279-283.

Dygert S., L. H. Li, D. Florida, J. A. Thoma, Anal. Biochem. 13 (1965) 367-374.

Lowry O. H., N. J. Rosebrough, A. L. Farr, R. J. Randall, J. of Biol. Chem. 193 (1951) 265-275.

Basawaraj A. K., S. Manohar, J. Lalitha, Biotechnology and Bioprocess Engineering 18 (2013) 333-341.

Jean W. D., W. Y. Shieh, T. Y. Liu, Int. J. Syst. Evol. Microbiol. 56 (2006) 1245-1250.

Hosoya S., Jae-Hyuk Jang, Mina Yasumoto-Hirose, Satoru Matsuda and Hiroaki Kasai Int. J. Syst. Evol. Microbiol. 59 (2009) 1262-1266.

Andrykovitch G., I. Marx, App. Environ. Microbiol. 54 (1988) 1061-1062.

Lakshmikanth M., S. Manohar, Y. Souche, J. Lalitha, World J. Microbiol. Biotechnol. 22 (2006b) 1087-1094.

Morrice L. M., M. W. McLean, F. B. Williamson, W. F. Long, Eur. J. Biochem. 135 (1983) 553-558.

Bae M.C., J. Kim, H. S. Shim, D. S. Byun, D. M. Cho, and H. R. Kim (2003).

Vera J., R. Alvarez, E. Murano, J. C. Slebe, O. Leon, Appl. Environ. Microbiol. 64 (1998) 4378-4383.

Leon O., L. Quintana, G. Peruzzo, J. C. Slebe, Appl. Environ. Microbiol. 58 (1992) 4060-4063.

Sugano Y., I. Terada, M. Arita, M. Noma, T. Matsumoto, Appl. Environ. Mcrobiol. 59(5) (1993) 1549-54.

Kim B. J., H. J. Kim, S. H. Ha, S. H. Hwang, D. S. Byun, T. H. Lee, J. Y. Kong Biotechnol. Letters 21 (1999) 1011-1015.

Ziayoddin M., S. Manohar, J. Lalitha, J of Microbial and Biochemical Technology 4 (2012) 096-095.

Bakhtiari M. R., M. G. Faezi, M. Fallahpour, A. Noohi, N. Moazami, Z. Amidi, Process Biochem. 41 (2006) 547-551.

Krishna P. K., M. S. Venkata, R. R. Sreenivas, R. P. Bikas, P. N. Sarma, Biochem. Eng. J. 24 (2005) 17-26.

Sreenivas R. R., R. S. Prakasham, P. K. Krishna, S. Rajesham, P. N. Sarma, R. L. Venkateswar, Proc. Biochem. 39 (2004) 951-956.

Araki T., M. Hayakawa, L. Zhang, S. Karita, T. Morishita, J. Mar. Biotechnol. 6 (1998) 260-265.

Wang J., X. Jiang, H. Mou, H. Guan, J. Appl. Phyco. 16(5) (2004) 333-340.

Aoki T., T. Araki, M. Kitamikado, Eur. J. Biochem. 187 (1990) 461- 465.

Fu X. T., H. Lin, S. M. Kim, Appl. Microbiol. Biotechnol. 78 (2008) 265-273.

Wang J., H. Mou, X. Jiang, H. Guan, Appl. Microbiol. Biotechnol. 71 (2006) 833-839.

Lakshmikanth M., S. Manohar, J. Lalitha, Proc. Biochem. 44 (2009) 999-1003. ( Received 22 June 2014; accepted 29 June 2014 ).

Show More Hide
Cited By:

[1] P. Rajkumar, R. Venkatesan, S. Sasikumar, T. Ramprasath, P. Karuppiah, A. Ramu, G. Selvam, "Characterization of agarolytic enzymes of Arthrobacter spp. AG-1 for the whole cell conversion of agar into 3,6-anhydro-α- l -galactose in one pot", Process Biochemistry, Vol. 69, p. 52, 2018

DOI: https://doi.org/10.1016/j.procbio.2018.03.017