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International Letters of Natural Sciences
Volume 17

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Evaluation of Antiepileptic Activity of Methanolic Leaves Extract of Tragia involucrata Linn. in Mice

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Abstract:

The present investigation was aimed to study an antiepileptic activity of methanolic extract of Tragia involucrata Linn in mice. In vivo screening models like maximal electroshock-induced convulsion (MES), pentylenetetrazole (PTZ) and picrotoxin (PTX) induced models are used to evaluate the antiepileptic effects of the extracts. The biochemical estimation was done by measuring the lipid peroxidation and reduced glutathione (GSH). In the MES induced convulsion, methanolic extract of Tragia involucrata (METI) at high dose (800 mg/kg body weight), showed high significant inhibition on tonic hind limb extension (THLE, 6.83 ±0.30***) and decrease in duration of stupor period (108.7 ±6.53***). In PTZ and PTX induced model METI (400 mg/kg and 800 mg/kg) showed significant delay on the onset of convulsions, decreased duration of convulsion and reduced mortality significantly. It also showed significant decrease in brain MDA level in lipid peroxidation profile, and increase in the brain glutathione levels in mice against PTZ induced convulsion. The results confirmed that Tragia involucrata Linn possesses dose dependent antiepileptic activity.

Info:

Periodical:
International Letters of Natural Sciences (Volume 17)
Pages:
167-179
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.17.167
Citation:
G. G. Varma et al., "Evaluation of Antiepileptic Activity of Methanolic Leaves Extract of Tragia involucrata Linn. in Mice", International Letters of Natural Sciences, Vol. 17, pp. 167-179, 2014
Online since:
Jun 2014
Authors:
Ganapathi G. Varma, Benson K. Mathai, Kuntal Das, Girish Gowda, S. Rammohan, John Wilking Einstein
Keywords:
Anticonvulsant Activity, Maximal Electroshock, Pentylenetetrazole, Picrotoxin, Tragia involucrate
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Distribution:
Open Access

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This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

Asl, M.A., S.S. Rad, and F. Zamansoltani, 2007. Anticonvulsant effects of aerial parts of passiflora incarnata extract in mice: involvement of benzodiazepine and opioid receptors. BMC. Complement. Altern. Med., 7: 1-6.

Ayyanar, M. And S. Ignacimuthu, 2005. Traditional knowledge of Kani tribals in Kouthalai of Tirunelveli hills, Tamil Nadu, India. J. Ethnopharmacol., 102: 246-255.

Debnath, S., M. Kannadasan, A. Acharjee, C, Bhattacharjee, S. Kumar, and G.G. Kumar, 2010. Antioxidant activity of the hydro-alcoholic extract of Erythrina fusca Lour. bark against the animal models of epilepsy. J. Chem. Pharm. Res., 2: 379-383.

Dhara, A.K., V. Suba, T. Sen, S. Pal, and A.K. Chaudhary, 2000. Preliminary studies on anti inflammataoray and analgesic activity of the methanolic fraction of root extract of Tragia involucrata Linn. J. Ethanopharmacol., 72: 265-268.

Dhara, A.K., S. Pal, and A.K. Chaudhary, 2002. Psychopharmacological Studies on Tragia involucrata Root Extract. Phtother. Res. 16: 326-330.

Ezekiel, I., M.A. Mabrouk, J.O. Ayo, A.D. Goji, A.O. Okpanachi, A. Mohammed, and Y. Tanko, 2010. Study of the Effect of Hydro-Ethanolic Extract of Commiphora Africana (Stem-bark) on Sleeping Time and Convulsion in Mice. Asian. J. Med. Sci., 2: 85-88.

Gupta, Y.K., S. Briyal, and M. Sharma, 2009. Protective effect of curcumin against kainic acid induced seizures and oxidative stress in rats. Ind. J. Physiol. Pharmacol., 53: 39-46.

Hema, B., S. Bhupendra, T.S. Mohamed Saleem, and K. Gauthaman, 2009. Anticonvulsant Effect of Drosera burmannii Vahl. Int. J. Applied. Res. Nat. Prod., 2: 14.

Joy, P.P., J. Thomas, S. Mathew, and B.P. Skaria, 2001. Medicinal Plants Tropical Horticulture. Vol. 2., Calcutta: Naya Prokashan.

Kar, A., B.K. Choudhary, and N.G. Bandyopadhyay, 2003. Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats. J Ethnopharmacol., 84: 105-108.

Khandelwal, K.R., 1996. Practical pharmacognosy techniques and experiments. 3rd ed. Pune: Nirali Prakashan.

Kirtikar, K.R. and B. D. Basu, 1977. Indian medicinal plants. 2nd ed, Vol. 1., Dehradun: International Book Publishers.

Kumar, A.S. and R. Gandhimathi, 2010. Effect of Guettarda speciosa extracts on antioxidant enzymes levels in rat brain after induction of seizures by MES and PTZ. J. Nat. Prod., 3: 80-85.

Mac, T.L., D.S. Tran, F. Quet, P. Odermatt, P.M. Preux, and C.T. Tan, 2007. Epidemiology, aetiology, and clinical management of epilepsy in Asia: a systematic review. Lancet. Neurol., 6: 533-543.

Mahomed, I.M. and J.A. Ojewole, 2006. Anticonvulsant activity of Harpagophytum procumbens DC (Pedaliaceae) secondary root aqueous extract in mice. Brain. Res. Bull., 69: 57-62.

Medappa, N., 2001. Developments In Mental Health Scenario: Need To Stop Exclusion - Dare To Care. ICMR Bulletin; 2001; New Delhi. Indian Council of Medical Research.

Ohkawa, H., N. Ohishi, and K. Yagi, 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 35l-358.

Olatokunboh, A.O., Y.O. Kayode, and O.K. Adeola, 2009. Anticonvulsant activity of Rauvolfia Vomitoria (Afzel). Afric. J. Pharm. Pharmacol., 3: 319-322.

Palani, S., S. Nirmal Kumar, R. Gokulan, D. Rajalingam, and B. Senthil Kumar, 2009. Evaluation of Nephroprotective and antioxidant potential of Tragia involucrate. Drug Invention Today., 1: 55-60.

Park, H.G., S.Y. Yoon, J.Y. Choi, G.S. Lee, J.H. Choi, C.Y. Shin, 2007. Anticonvulsant effect of wogonin isolated from Scutellaria baicalensis. Eur. J. Pharmacol., 574: 112119.

Pourgholami, M.H., S. Majzoob, M. Javadi, M. Kamalinejad, G.H. Fanaee, and M. Sayyah, 1999. The fruit essential oil of Pimpinella anisum exerts anticonvulsant effects in mice. J. Ethnopharmacol., 66: 211-215.

Rahmatullah, M., A.T. Kabir, M. Rahman, S. Hossan, Z. Khatun, A. Khatun, 2010. Ethnomedicinal Practices among a Minority Group of Christians Residing in Mirzapur Village of Dinajpur District, Bangladesh. Adv. In. Nat. Appl. Sci., 4: 25-51.

Raza, M., F. Shaheen, M.I. Choudhary, S. Sombati, 2001. Anticonvulsant activities of ethanolic extract and aqueous fraction isolated from Delphinium denudatum. J. Ethnopharmacol., 78: 73-78.

Rout, S.K. and D.M. Kar, 2010. A review on antiepileptic agents, current research and future prospectus on conventional and traditional drugs. Int. J. Pharm. Sci. Rev. Res., 3: 19-23.

Samy, R.P., P. Gopalakrishnakone, M. Sarumathi, and S. Ignacimuthu, 2006. Wound healing potential of Tragia involucrata extract in rats. Fitotherapia., 77: 300-302.

Samy, R.P., S. Ignacimuthu, and A. Sen, 1998. Screening of 34 Indian medicinal plants for antibacterial properties. J Ethnopharmacol., 62: 173-182.

Sarada, S., G.S. Nair, and B.R. Reghunath, 2002. Quantification of medicinally valuable weeds in oil palm plantations of Kerala. J. Trop. Agric., 40: 19-26.

Savithramma, N., Sulochana, K.N. Rao. 2007. Ethnobotanical survey of plants used to treat asthma in Andhra Pradesh, India. J Ethnopharmacol., 113: 54-61.

Sedlak, J., R.H. Lindsay, 1968. Estimation of total protein bound and non protein bond sulphydryl group in tissue with Ellman's reagent. Anal. Biochem., 25: 192-197.

Silja, V.P., K.S. Varma, and K.V. Mohanan, 2008. Ethanomedical plant knwoledge of the Mullu kuruma tribe of Wayanad district, Kerala. Ind. J. Trad. Knowl., 7: 604-612.

Surapaneni, K.M., 2007. Status of Lipid Peroxidation, Glutathione, Ascorbic Acid, Vitamin E and Antioxidant Enzymes in Schizophrenic Patients. J. Clin. Diag. Res., 1: 39-44.

Swinyard, E.A., W.C. Brown, and L.S. Goodman, 1952. Comparative assays of antiepileptic drugs in mice and rats. J. Pharmacol. Exp. Ther., 106: 319-330.

Trease, G.E. and M.C. Evans, 1983. Text book of Pharmacognosy. London: Bailliare Tindall. 12: pp: 193.

Udayan, P.S., M.K. Harinarayanan, K.V. Tushar, and I. Balachandran, 2008. Some common used by Kurichiar tribes of Tirnelli forest, Wayanad district, Kerala in medicine and other traditional uses. Ind. J. Trad. Knowl., 7: 250-255.

Varija, D., K.P. Kumar, K.P. Reddy, and V.K. Reddy, 2009. Prolonged constriction of sciatic nerve affecting oxidative stressors & antioxidant enzymes in rat. Ind. J. Med. Res., 129: 587-592.

Vasconcelos, S.M., N.M. Lima, G.T. Sales, G.M. Cunha, L.M. Aguiar, E.R. Silveira, 2007. Anticonvulsant activity of hydroalcoholic extracts from Erythrina velutina and Erythrina mulungu. J. Ethnopharmacol., 110: 271-274.

Vogel, H. Gerhard, H. Vogel Wolf gang, 2002. (Eds) Drug discovery and Evaluation pharmacological assays (Springer). 2: (E) 424.

Wannang, N.N., J.A. Anuka, H.O. Kwanashie, S.S. Gyang, and A. Auta, 2008. Antiseizure activity of the aqueous leaf extract of Solanum nigrum linn (solanaceae) in experimental animal. Afr. Health. Sci., 8: 74-79.

Yaua, J., A.H. Yaro, M.S. Abubakarb, J.A. Anukaa, and I.M. Hussainia, 2008. Anticonvulsant activity of Carissa edulis (Vahl) (Apocynaceae) root bark extract. J. Ethnopharmacol., 120: 255-258. ( Received 19 June 2014; accepted 28 June 2014 ).

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