Effect of Thyroxin Induction on Enzymatic and Biochemical Levels in Albino Rats

Hanchinalmath, Jayashree V.; Shivasharanappa, Kirankumar; Roopa, T.S.; Saisundeep, Y.

doi:10.18052/www.scipress.com/ILNS.13.91

ILNS > Volume 13 > Effect of Thyroxin Induction on Enzymatic and...

< Back to Volume

Effect of Thyroxin Induction on Enzymatic and Biochemical Levels in Albino Rats

Full Text PDF

Abstract:

The albino Wistar rats were used to investigate the effect of thyroxine administration on the activity of citric acid, α-Amylase, creatine phosphokinase (CPK), creatinine, cholesterol and triglycerides level in blood serum and citric acid in liver and kidney. Two groups of rats were taken for the experiment. One group was injected subcutaneously with 200 μl of thyroxine/50 g of body weight, the other group served as control. The animals were vivisected with anesthesia after intervals of 24, 48, 72 and 96 hours α-Amylase and CPK levels in blood; citric acid level in liver and kidney of control and thyroxine treated rats. The results showed that the α-Amylase activity remained unaltered (100 g/h/l) throughout the experiment. However, citric acid level found to be 460 mg in the liver and 415 mg in kidney of control rats. The values slightly increased to 480 and 680 mg in respective tissues of thyroxine treated animals for 24 hr. The values decreased in 48 and 72 hours of treatment. Further the values found to be increased after 96 hours of treatment. Although the thyroxine treatment altered citric acid, Creatinine, Cholesterol, Triglycerides and CPK levels in the tissues, the serum α-Amylase was not influenced by the thyroxine. The study suggests that the thyroxine acts as a regulator all the above parameters in the cellular level.

Info:

Periodical:

International Letters of Natural Sciences (Volume 13)

Pages:

91-99

DOI:

https://doi.org/10.18052/www.scipress.com/ILNS.13.91

Citation:

J. V. Hanchinalmath et al., "Effect of Thyroxin Induction on Enzymatic and Biochemical Levels in Albino Rats", International Letters of Natural Sciences, Vol. 13, pp. 91-99, 2014

Online since:

April 2014

Authors:

Jayashree V. Hanchinalmath, Kirankumar Shivasharanappa, T.S. Roopa, Y. Saisundeep

Keywords:

CPK, Kidney, Thyroxine, α-Amylase

Export:

RIS, BibTeX, Text

Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

Walker, Science 204 (1979) 427-429.

Settimio G., Jacob R., Harold E., Biochemistry 24(14) (1985) 3771-3776.

Shainberg G. Yagil, Yaffe D., Dev Biol 25(1) (1971) 1-29.

Yaffe O. Saxel, Nature 270 (1992) 725-727.

Penniston K. L., Nakada S. Y., Holmes R. P., Assimos D. G., Journal of Endourology 22(3) (2008) 567.

Holloszy H. O., Winder W. W., Am. J. Physiol. 236(3) (1979) 180-183.

Stroev E. A., Makarova V. G., In, Rassadin BV. Physicochemical studies of protein properties. Laboratory Manual in Biochemistry; Moscow: Mir Publishers; 1989; 45-47.

Fabing D. L., Ertinghausen G., Clin Chem 17 (1971) 391.

Trinder P., Annals of Clinical Biochemistry 6 (1969) 24-27.

Natelson S., Lugovoy J. K., Pincus J. B., J. Biol. Chem 170 (1947) 597.

Schibler M. Tosi, Pittet A. C., Fabiani L., Wellauer P. K., J Biochem 95(3) (1984) 697702.

Frank Dickens, Biochem J 35(8-9) (1941) 1011-1023.

Krebs H. A., Perspect Biol. Med 14 (1970) 154-170.

Wittenberger T., Schaller H. C., Hellebrand S., J. Mol. Biol 307 (2001) 799-813.

Mcleish M. J., Kenyon G. L., Crit. Rev. Biochem. Mol. Biol 40(1) (2005) 1-20.

Schlattner U., Tokarska-schlattner M., Wallimann T., Biochim Biophys Acta 1762 (2) (2006) 164-180.

Wallimann T., Wyss M., Brdiczka D., Nicolay K., Eppenberger H. M., Biochem J 281(1) (1992) 21-40.

Elder, Ann-Clin-Biochem 27(2) (1990) 110-3.

Fried M., Abramson S., Meyer J. H., Digestive Diseases and Sciences 32 (1987) 10971103. ( Received 03 April 2014; accepted 10 April 2014 ).

Show More Hide

Cited By:

This article has no citations.