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Genetic Variation in Mutants of Chilli (Capsicum annum) Revealed by RAPD Marker

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Abstract:

The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands

Info:

Periodical:
International Letters of Natural Sciences (Volume 11)
Pages:
1-8
DOI:
https://doi.org/10.18052/www.scipress.com/ILNS.11.1
Citation:
L. Mullainathan et al., "Genetic Variation in Mutants of Chilli (Capsicum annum) Revealed by RAPD Marker", International Letters of Natural Sciences, Vol. 11, pp. 1-8, 2014
Online since:
Feb 2014
Authors:
L. Mullainathan, A. Sridevi, S. Umavathi, E. Sanjai Gandhi
Keywords:
Chilli, EMS, Induced Mutation, RAPD Analysis
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Distribution:
Open Access

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This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

Arulbalachandran D, 2006. Effect of physical and chemical mutagenesis in black gram (Vigna mungo (L. ) Hepper). Ph.D. Thesis, Faculty of Science, Annamalai University, Annamalai Nagar, India.

Ashok Kumar G., V. Ponnuswami, Research Journal of Agricultural Sciences 1(3) (2010) 193-195.

Atienzar F. A., P. Venier, A. N. Jha, M. H. Depledge., Mut. Res. Gen. Tox. Environ. 521(1-2) (2002) 151-163.

Basu S. K., A. K. De, 2003. Capsicum: Historical and Botanical Perspectives. Citation information Capsicum. The genus Capsicum Print ISBN: 978-0-415- 29991-6, eBook ISBN: 978-0-203-38115.

Bosland P. W., F. J. Votava, Peppers, Vegetable and Spice Capsicums. CABI Publishing, 2000, p.204.

Doyle J. J., J. L. Doyle, Focus 12 (1990) 13-14.

Jaccard P., Bull. Soc. Vaud. Sci. Nat. 44 (1908) 223-270.

Naugžemys D., S. Zilinskaite, J. Denkovskij, J. Patamsytė, J. Literskis, D. Zvingila, Biologia 53(3) (2007) 34-39.

Salvador M. H., Genetic resources of chilli (Capsicum spp. ) in Mexico. Proc. of the 16th Int. Pepper Conf., Tampico, Tamaulipas, Mexico, November, 2002, pp.10-12.

Senthamizh Selvi B., V. Ponnuswami, P. S. Kavitha, Adv. Nat. Appl. Sci. 2(3) (2008) 129-134.

Subramanian V., Gurtu S., Nageswara Rao, Nigam S. N., Genome 43 (2000) 656-660.

Wachira F. N., Waugh R., Hackett C. A., Powell W., Genome 38 (1995) 201-210.

Williams J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, S. V. Tingey, Nucleic Acids Res. 18 (1990) 6531-6535.

Yüzbasıoğlu E., S. Özcan, L. Açık, Genet. Resour. Crop Ev. 53 (2006) 507-514.

E. Sanjai Gandhi, A. Sri Devi, International Letters of Natural Sciences 5 (2014) 18-23. ( Received 05 February 2014; accepted 16 February 2014 ).

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