Study of the Interaction of Ciprofloxacin and Sparfloxacin with Biomolecules by Spectral, Electrochemical and Molecular Docking Methods

Rajendiran, N.; Suresh, M.

doi:10.18052/www.scipress.com/ILCPA.78.1

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Study of the Interaction of Ciprofloxacin and Sparfloxacin with Biomolecules by Spectral, Electrochemical and Molecular Docking Methods

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Abstract:

Interactions of ciprofloxacin and sparfloxacin with different biomolecules (DNA, RNA and BSA) are investigated by UV–Visible spectroscopy, fluorescence spectroscopy, cyclic voltammetry and molecular docking methods. Upon increasing the concentration of the biomolecules, the absorption maxima of ciprofloxacin and sparfloxacin are red shifted in the aqueous solutions whereas red or blue shift noticed in the fluorescence spectra. The negative free energy changes suggest that the interaction processes are spontaneous. Cyclic voltammetry results suggested that when the drug concentration is increased, the anodic electrode potential increased. Molecular docking results showed that hydrophobic forces, electrostatic interactions, and hydrogen bonds played vital roles in the interaction drugs with biomolecules. The molecular docking calculation clarifies the binding mode and the binding sites are in good accordance with the experiment results.

Info:

Periodical:

International Letters of Chemistry, Physics and Astronomy (Volume 78)

Pages:

1-29

DOI:

https://doi.org/10.18052/www.scipress.com/ILCPA.78.1

Citation:

N. Rajendiran and M. Suresh, "Study of the Interaction of Ciprofloxacin and Sparfloxacin with Biomolecules by Spectral, Electrochemical and Molecular Docking Methods", International Letters of Chemistry, Physics and Astronomy, Vol. 78, pp. 1-29, 2018

Online since:

April 2018

Authors:

N. Rajendiran, M. Suresh

Keywords:

Biosensing, BSA, Ciprofloxacin, DNA, RNA, Sparfloxacin

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Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

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[3] P. Ipte, A. Satpati, "Probing the interaction of ciprofloxacin and E. coli by electrochemistry, spectroscopy and atomic force microscopy", Biophysical Chemistry, p. 106456, 2020

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[4] T. Khalil, A. El‐Dissouky, D. Al‐Wahaib, N. Abrar, D. El‐Sayed, "Synthesis, characterization, antimicrobial activity, 3D‐QSAR, DFT, and molecular docking of some ciprofloxacin derivatives and their copper(II) complexes", Applied Organometallic Chemistry, 2020

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[5] P. Ipte, A. Sharma, H. Pal, A. Satpati, "Probing the interaction of ciprofloxacin with dsDNA: Electrochemical, spectro-electrochemical and AFM investigation", Journal of Electroanalytical Chemistry, Vol. 885, p. 115098, 2021

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