Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILCPA > Volume 75 > Extractive Spectrophotometric Methods for...
< Back to Volume

Extractive Spectrophotometric Methods for Determination of Chlorpheniramine Maleate in Pure Form, Pharmaceutical Preparations and Biological Fluids

Full Text PDF

Abstract:

In this study a simple, rapid and sensitive spectrophotometric method was developed for the determination of an antihistaminic drug chlorpheniramine maleate (CPM) in pure form, pharmaceutical preparations, spiked humane urine and spiked blood serum. This method was based on the formation of ion-pairs between the basic nitrogen of the CPM drug and four chromogenic reagents namely bromocresol purple (BCP), alizarine Red S (ARS), eriochrome cyanine R (ECR), and cresol red (CR). The extracted colored ion-pairs were measured spectrophotometrically at 390, 425, 503 and 408 nm for BCP, ARS, ECR and CR reagents, respectively. The different parameters that affect the color development between CPM drug and dyestuff reagents were extensively studied to determine the optimal conditions for the assay procedure. The reaction was studied as a function of the volume of reagents, nature of solvent, temperature, reaction time and stoichiometric ratio between the CPM drug and the reagents. Beer’s law was valid over the concentration ranges of 1-30, 1-10, 2-120 and 4-120 μg mL-1 of CPM drug using BCP, ARS, ECR and CR reagents, respectively. The Sandell sensitivity, molar absorptivity, limit of detection and limit of quantification were determined. Applications of the proposed procedure to the analysis of the pharmaceutical preparations, spiked humane urine and spiked blood serum gave reproducible and accurate results without any interference from excipients. The results obtained by the proposed method were in good agreement with those obtained by reported method. The method can be suggested for the routine analysis of the cited drug.

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 75)
Pages:
11-24
Citation:
M. E. B. Mohamed et al., "Extractive Spectrophotometric Methods for Determination of Chlorpheniramine Maleate in Pure Form, Pharmaceutical Preparations and Biological Fluids", International Letters of Chemistry, Physics and Astronomy, Vol. 75, pp. 11-24, 2017
Online since:
August 2017
Export:
Distribution:
References:

[1] M.M. Abdulbari, M.S. Ihsan, Simultaneous determination and validation of chlorpheniramine maleate, acetaminophen, phenylpropanolamine hydrochloride and caffeine in tablet dosage form by using reverse phase high performance liquid chromatography (RP-HPLC), Int. J. Pharm. Sci. 5 (2013).

[2] A. Kaura et al., Spectrophotometric determination of chlorpheniramine maleate and phenylpropanolamine hydrochloride in dosage forms, Int. Curr. Pharm. J. 2(5) (2013) 97-100.

DOI: https://doi.org/10.3329/icpj.v2i5.14436

[3] B. Rushikesh, N. Atul, S. Sanjay, Analytical method development and validation of phenylephrine hydrochloride, chlorpheniramine maleate, paracetamol and caffeine in bulk drug and tablet dosage form by RP-HPLC, Indoamerican. J. Pharm. Res. 3 (2013).

[4] M. Maithani et al., Development and validation of a RP-HPLC method for the determinationof chlorpheniramine maleate and phenylephrine in pharmaceutical dosage form, Pharm. Glob. Int. J. Comp. Pharm. 5 (2010) 0976.

[5] V.K. Redasani et al., Simultaneous determination of chlorpheniramine maleate, phenylephrine hydrochloride, paracetamol and caffeine in pharmaceutical preparation by RP-HPLC, Chem. Ind. Chem. Eng. Q. 19 (2013) 57−65.

DOI: https://doi.org/10.2298/ciceq120302042r

[6] S.Z. Hamide, Ö. Tuncel, Simultaneous high-performance liquid chromatographic determination of paracetamol, phenylephrine HCl, and chlorpheniramine maleate in pharmaceutical dosage forms, J. Chromatogr. Sci. 40(2) (2002) 97-100.

DOI: https://doi.org/10.1093/chromsci/40.2.97

[7] R. Sandeep, Simultaneous determination of chlorpheniramine maleate paracetamol and pseudoephedrine hydrochloride in pharmaceutical preparations by HPLC, Int. J. Life Sci. Pharma Res. 1 (2011) 94-100.

[8] P. Ozan, S. Murat, O. Tuncel, Simultaneous determination of paracetamol, phenylephrine hydrochloride, oxolamine citrate and chlorpheniramine maleate by HPLC in pharmaceutical dosage forms, J. Chem. 8(3) (2011) 1275-1279.

DOI: https://doi.org/10.1155/2011/454325

[9] M. Kazemipour, M. Ansari, Derivative spectrophotometry for simultaneous analysis of chlorpheniramine maleate, phenylephrine HCl and phenylpropanolamine HCl in ternary mixtures and pharmaceutical dosage forms, Iran. J. Pharm. Res. 3 (2005) 147-153.

[10] A.K. Kaura et al., Spectrophotometric determination of chlorpheniramine maleate and phenylpropanolamine hydrochloride, by two wavelengths method, J. Pharm. Res. 7 (2013) 404-408.

DOI: https://doi.org/10.1016/j.jopr.2013.04.043

[11] B. Anupam, Spectrophotometric method for determination of chlorpheniramine maleate in pharmaceutical Preparations in the presence of codeine phosphate and ephedrine hydrochloride, Analyst. 105 (1980) 353-358.

DOI: https://doi.org/10.1039/an9800500353

[12] S.J. Wadher, T.M. Kalyankar, P.P. Panchal, Development and validation of simultaneous estimation of chlorpheniramine maleate and phenylephrine hydrochloride in bulk and capsule dosage form by ultra-violet spectrophotometry, Int. Journal Chem. Tech. Res. 5(5) (2013).

[13] B. Mohammed, Visible spectrophotometric determination of chlorpheniramine maleate and diphenhydramine hydrochloride in raw and dosage form using potassium permanganate, Orient. J. Chem. 32 (2016) 885-894.

DOI: https://doi.org/10.13005/ojc/320214

[14] A.E.E. Ibrahim et al., Chemometric optimization of sequential injection spectrophotometric method for chlorpheniramine determination in pharmaceutical formulations, J. Flow Injection Anal. 27(1) (2010) 26–31.

[15] S. Ulu, Z. Aydogmus, A new spectrophotometric method for the determination of tianeptine in tablets using ion-pair reagents, Chem. Pharm. Bull. 56(12) (2008) 1635-1638.

DOI: https://doi.org/10.1248/cpb.56.1635

[16] S. Sahu et al., Ion-pair spectrophotometric estimation of gemifloxacin, Pharm. Methods. 3(1) (2012) 26–30.

[17] M.N. Patel et al., Spectrophotometric determination of ciprofloxacin by ion pair formation, J. Anal. Chem. 67(7) (2012) 655–660.

[18] M.I. Walash et al., Spectrophotometric determination of tizanidine and orphenadrine via ion pair complex formation using eosin Y insight, Chemistry Central Journal. 5(1) (2011) 60.

DOI: https://doi.org/10.1186/1752-153x-5-60

[19] E.Y.Z. Frag et al., The use of integrated analytical tools for determination of cyclopentolate and naphazoline hydrochlolrides in pure and pharmaceutical preparations, Pharm. Anal. Acta. 2(1) (2011).

DOI: https://doi.org/10.4172/2153-2435.1000114

[20] Y.Z.F. Eman, G.M. Gehad, M.S.A. Hanan, Utility of ion-associate formation reactions for the spectrophotometric determination of sildenafil citrate in pure form and in Virecta tablets, Pharm. Anal. Acta. 2 (2011) 1-7.

DOI: https://doi.org/10.4172/2153-2435.1000131

[21] R. Nafisur, K. Asmaa, R. Habibur, Studies on the development of spectrophotometric method for the determination of haloperidol in pharmaceutical preparations, Quím. Nova. 35 (2012) 392-397.

DOI: https://doi.org/10.1590/s0100-40422012000200028

[22] H.M. Elqudaby et al., Extractive spectrophotometric method for determination of meoxipril HCl and perindopril in raw materials and tablets using ion pair formation, Int. J. Chem. Anal. Sci. 3 (2012) 1334-1339.

[23] M.M. Khalil et al., Spectrophotometric studies using ion-pair formations of Ranitidine hydrochloride in pure and in Pharmaceutical forms with some dyestuff reagents, J. Appl. Pharm. Sci. 3 (2013) 92-98.

[24] I.S. Balogh et al., A spectrophotometric method for manganese determination in water samples based on ion pair formation and dispersive liquid-liquid microextraction, Int. J. Environ. Anal. Chem. 92(9) (2012) 1059-1071.

DOI: https://doi.org/10.1080/03067319.2010.537750

[25] F. Antonio, R. Raffaele, Modification of Job's method for determining the stoichiometry of protein–protein complexes, Anal. Biochem. 313(1) (2003) 170–172.

[26] G. Mehmet, A. O. Sibel, The role of and the place of method validation in drug analysis using electroanalytical techniques, The Open Anal. Chem. J. 5 (2011) 1-21.

[27] A. Shrivastava, V. Gupta, Methods for the determination of limit of detection and limit of quantitation of the analytical methods, Chronicles of Young Scientists. 2(1) (2011) 21-25.

DOI: https://doi.org/10.4103/2229-5186.79345
Show More Hide