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Development of the UV Spectrophotometric Method of Azithromycin in API and Stress Degradation Studies

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Azithromycin (AZI) is a semi-synthetic macrolide antibiotic drug, effective against a wide variety of bacteria. The present study describes a simple, accurate, reproducible and precise UV Spectrophotometric method for the estimation of AZI (pH 6.8 Phosphate buffer). The absorbance maximum (λmax) for AZI was found to be 208nm. The method reveals high sensitivity, with linearity in the 10 µg/ml to 50 µg/ml range. The lower limit of detection was found to be 1.6µg/ml and the limit of quantification was found to be 5µg/ml. All the calibration curves demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient higher than 0.99. The % recovery was found to be 99.72%. AZI was also subjected to stress degradation under different conditions recommended by the International Conference on Harmonization (ICH).


International Letters of Chemistry, Physics and Astronomy (Volume 68)
S. Bhimani et al., "Development of the UV Spectrophotometric Method of Azithromycin in API and Stress Degradation Studies", International Letters of Chemistry, Physics and Astronomy, Vol. 68, pp. 48-53, 2016
Online since:
July 2016

[1] D. Amantea, M. Certo, F. Petrelli, C. Tassorelli, G. Micieli, M. T. Corasaniti, P. Puccetti, F. Fallarino and G. Bagetta, Azithromycin protects mice against ischemic stroke injury by promoting macrophage transition towards M2 phenotype, Exp. Neurol. 275 Pt 1 (2016).

[2] S. Piscitelli, L. Danziger and K. Rodvold, Clarithromycin and azithromycin: new macrolide antibiotics, Clin. Pharm. 11 (1992) 137.

[3] G. Foulds, R. M. Shepard and R. B. Johnson, The pharmacokinetics of azithromycin in human serum and tissues, J. Antimicrob. Chemother. 25 Suppl A (1990) 73-82.

[4] Z. Mandić, Z. Weitner and M. Ilijaš, Electrochemical oxidation of azithromycin and its derivatives, J. Pharm. Biomed. Anal. 33 (2003) 647-654.

[5] B. Nigović and B. Šimunić, Voltammetric assay of azithromycin in pharmaceutical dosage forms, J. Pharm. Biomed. Anal. 32 (2003) 197-202.

[6] R. Gandhi, C. L. Kaul and R. Panchagnula, Validated LC method for in-vitro analysis of azithromycin using electrochemical detection, J. Pharm. Biomed. Anal. 23 (2000) 1073-1078.

[7] Z. Y. Yang, L. Wang and X. Tang, Determination of azithromycin by ion-pair HPLC with UV detection, J. Pharm. Biomed. Anal. 49 (2009) 811-815.

[8] P. Zubata, R. Ceresole, M. A. Rosasco and M. T. Pizzorno, A new HPLC method for azithromycin quantitation, J. Pharm. Biomed. Anal. 27 (2002) 833-836.

[9] P. L. Raval, F. A. Mehta, K. B. Ahir and K. K. Bhatt, Simultaneous estimation of azithromycin dihydrate and cefixime trihydrate in pharmaceutical formulation by HPTLC method, J. Liq. Chromatogr. Rel. Technol. 37 (2014) 1805-1818.

[10] B. M. Chen, Y. Z. Liang, X. Chen, S. G. Liu, F. L. Deng and P. Zhou, Quantitative determination of azithromycin in human plasma by liquid chromatography-mass spectrometry and its application in a bioequivalence study, J. Pharm. Biomed. Anal. 42 (2006).

[11] R. V. S. Nirogi, V. N. Kandikere, M. Shukla, K. Mudigonda, S. Maurya, R. Boosi and A. Yerramilli, Sensitive and selective liquid chromatography–tandem mass spectrometry method for the quantification of azithromycin in human plasma, Anal. Chim. Acta 553 (2005).

[12] Y. Shen, C. Yin, M. Su and J. Tu, Rapid, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of topically applied azithromycin in rabbit conjunctiva tissues, J. Pharm. Biomed. Anal. 52 (2010).

[13] ICH, Validation of analytical procedures: text and methodology, Q2 (R1) 1 (2005).

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