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Development of the UV Spectrophotometric Method of Azithromycin in API and Stress Degradation Studies

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Abstract:

Azithromycin (AZI) is a semi-synthetic macrolide antibiotic drug, effective against a wide variety of bacteria. The present study describes a simple, accurate, reproducible and precise UV Spectrophotometric method for the estimation of AZI (pH 6.8 Phosphate buffer). The absorbance maximum (λmax) for AZI was found to be 208nm. The method reveals high sensitivity, with linearity in the 10 µg/ml to 50 µg/ml range. The lower limit of detection was found to be 1.6µg/ml and the limit of quantification was found to be 5µg/ml. All the calibration curves demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient higher than 0.99. The % recovery was found to be 99.72%. AZI was also subjected to stress degradation under different conditions recommended by the International Conference on Harmonization (ICH).

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 68)
Pages:
48-53
DOI:
10.18052/www.scipress.com/ILCPA.68.48
Citation:
S. Bhimani et al., "Development of the UV Spectrophotometric Method of Azithromycin in API and Stress Degradation Studies", International Letters of Chemistry, Physics and Astronomy, Vol. 68, pp. 48-53, 2016
Online since:
Jul 2016
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