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International Letters of Chemistry, Physics and Astronomy
ILCPA Volume 61

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Spectrophotometric Determination of Selenium in Various Samples Using 5,6-Diaminouracil Hydrochloride (DAUH)

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A simple, rapid and sensitive spectrophotometric method was developed for the determination of Se (IV) using 5,6-diaminouracil hydrochloride (DAUH) as an analytical reagent. The reagent has been synthesized and characterized using IR, 1H NMR. The metal ion in aqueous medium forms yellow colored complex with DAUH showing maximum absorbance at 343 nm. Hence, analytical studies were further carried out at 343 nm. The reagent reacts with selenium in acidic medium to form yellow colored 1:1 (M:L) complex . The color reactions are instantaneous and absorbance values remain constant for one week. The composition of the Se (IV) complex with DAUH was studied by the method of job’s continuous variation and molar ratio method. Beer’s law was obeyed in the range 0.2-15.0 μg mL−1of Se (IV). The molar absorptivity and Sandell’s sensitivity of the method were found to be 0.5×10+4 0.031μ respectively. Since DAUH method is more sensitive, it was applied for the determination of selenium in samples.


International Letters of Chemistry, Physics and Astronomy (Volume 61)
S. F. Rassi, "Spectrophotometric Determination of Selenium in Various Samples Using 5,6-Diaminouracil Hydrochloride (DAUH)", International Letters of Chemistry, Physics and Astronomy, Vol. 61, pp. 128-136, 2015
Online since:
November 2015

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[1] U. Barache, A. Shaikh, T. Lokhande, M. Anuse, G. Kamble, V. Gurame, S. Gaikwad, "Acid switched efficient, cost effective, selective separation and determination of selenium(IV)", Journal of Environmental Chemical Engineering, Vol. 5, p. 4828, 2017