Spectrophotometric Determination of Selenium in Various Samples Using 5,6-Diaminouracil Hydrochloride (DAUH)

Rassi, Salwa Fares

doi:10.18052/www.scipress.com/ILCPA.61.128

ILCPA > ILCPA Volume 61 > Spectrophotometric Determination of Selenium in...

< Back to Volume

Spectrophotometric Determination of Selenium in Various Samples Using 5,6-Diaminouracil Hydrochloride (DAUH)

Full Text PDF

Abstract:

A simple, rapid and sensitive spectrophotometric method was developed for the determination of Se (IV) using 5,6-diaminouracil hydrochloride (DAUH) as an analytical reagent. The reagent has been synthesized and characterized using IR, 1H NMR. The metal ion in aqueous medium forms yellow colored complex with DAUH showing maximum absorbance at 343 nm. Hence, analytical studies were further carried out at 343 nm. The reagent reacts with selenium in acidic medium to form yellow colored 1:1 (M:L) complex . The color reactions are instantaneous and absorbance values remain constant for one week. The composition of the Se (IV) complex with DAUH was studied by the method of job’s continuous variation and molar ratio method. Beer’s law was obeyed in the range 0.2-15.0 μg mL⁻¹of Se (IV). The molar absorptivity and Sandell’s sensitivity of the method were found to be 0.5×10⁺⁴ L.mol^-1.cm^-1and 0.031μg.cm^-2 respectively. Since DAUH method is more sensitive, it was applied for the determination of selenium in samples.

Info:

Periodical:

International Letters of Chemistry, Physics and Astronomy (Volume 61)

Pages:

128-136

DOI:

https://doi.org/10.18052/www.scipress.com/ILCPA.61.128

Citation:

S. F. Rassi, "Spectrophotometric Determination of Selenium in Various Samples Using 5,6-Diaminouracil Hydrochloride (DAUH)", International Letters of Chemistry, Physics and Astronomy, Vol. 61, pp. 128-136, 2015

Online since:

November 2015

Authors:

Salwa Fares Rassi

Keywords:

5,6-Diaminouracil Hydrochloride (DAUH), Selenium Determination, Spectrophotometry

Export:

RIS, BibTeX, Text

Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] Holok N, Speechio J. J., Analyst. 199 (1994) 2179-2182.

[2] Manish R, Ramachandran K. N; Gupta V. K., Talanta. 41 (1994) 1623-1626.

[3] Agarwal D, Sunitha G; Gupta V. K., Indian. Chem. Soc. 73 (1998) 151-154.

[4] Safavi A, Afkhami A., Anal. Lett. 28 (1995) 1095-1101.

[5] Pathare M. N, Sawant A. D., Anal. Lett. 28(1995) 317-334.

[6] Pyrzynsaka K., Anal. Sci., 13(1997) 629-632.

[7] Revanasiddappa H. D, Kiran Kumar T. N., Anal. Sci., 17 (2001) 1309-1312.

[8] Bem E. M., Chem. Anal (Warsaw). 1979 24 155.

[9] Zingaro R. A. & W. C. Cooper, Selenium, Van Nostrand Reinhold, New York, 1974, 594.

[10] Rosenfeld J, Beath 0. A., Selenium. Academic Press, New York, (1964).

[11] Thomson CD, Eur. J. Clin Nutr., 58 (2004) 391-402.

[12] Goldhaber SB., Regulatory Toxicology and Pharmacology. 38(2003) 232-42.

[13] Combs G. F, J. r & Gray WP., Pharmacol . Ther., 79 (1998) 179-92.

[14] McKenzie R. C, Rafferty T. S, Beckett G. J., Immunol Today. 19(1998) 342-5.

[15] Levander O. A., J. Nutr., , 127(1997) 948S-50S.

[16] Arthur J. R, Can J Physiol Pharmacol. 69(1991) 1648-52.

[17] Patty F. A., Industrial Hygiene and Toxicology (Wiely, New York). 1962 2 886.

[18] Shamberger R. J., Biochemistry of Selenium (Plenum Press, NewYork). (1983).

[19] West P W & Shendrikar A. D., Anal Chim Acta. 1977 82 403.

[20] Shapira J. R., Organic Selenium Compound their Chemistry and Biology. (Wiely Interscience, NewYork) 1971 703.

[21] Jeffrey A. H, Roger G. B, Carolyn A, G, J AOAC Ibt. 1992 75 269.

[22] Stewart , Chow A., Talanta. 1993 40 1345.

[23] Hao D Q , Hang G. Z, Zhang Y. M , Tian G .J., Talanta. 1996 43 595.

[24] Macleod B. A , Shand C. A., Talanta. 1996, 43, 1091.

Show More Hide

Cited By:

[1] U. Barache, A. Shaikh, T. Lokhande, M. Anuse, G. Kamble, V. Gurame, S. Gaikwad, "Acid switched efficient, cost effective, selective separation and determination of selenium(IV)", Journal of Environmental Chemical Engineering, Vol. 5, p. 4828, 2017

DOI: https://doi.org/10.1016/j.jece.2017.09.021